Brazilian essential oil of Cymbopogon martinii: positive effects on inflammation-induced human fibroblasts and skin aging.

Dr. Carlos Rocha Oliveira
OrcID
Dr. Leonardo Mendes Bella
OrcID
Dr. Rodolfo de Paula Vieira
OrcID
Drª Marília Cristina Duarte
OrcID

    Dr. Carlos Rocha Oliveira

    Anhembi Morumbi University

    OrcID https://orcid.org/0000-0001-8634-2850

    Pharmacist - Biochemist, Master in Pharmacology and PhD in Biotechnology. Post-doctorate from the Institute of Pharmacology and Molecular Biology at UNIFESP. Professor of the Medicine course at Anhembi Morumbi University in São José dos Campos-SP and Supervising Professor in the Postgraduate Program in Biomedical Engineering (UNIFESP) in São José dos Campos-SP. He currently leads the Phytocomplexes and Cell Signaling Research Group (DGP/CNPq) and is coordinator of the Research Ethics Committee at Anhembi Morumbi University (CEP-UAM). He develops projects in the area of Pharmacology, with an emphasis on Pharmacology of Natural Products, working mainly on the following topics: cell signaling and mode of action of drugs, antibacterial and antioxidant activity of natural and herbal products. He has experience in developing projects in association with the pharmaceutical, food and cosmetics industries.

    Dr. Leonardo Mendes Bella

    Anhembi Morumbi University

    OrcID https://orcid.org/0000-0002-4099-5597

    Graduated in Pharmacy and Biochemistry from the Federal University of Juiz de Fora (2012), Master's degree in Pharmacy (Physiopathology and Toxicology) from the University of São Paulo (2014) and PhD in Pharmacy (Physiopathology and Toxicology) from the University of São Paulo (2018) and postgraduate degree in microbiology (2020) at Faculdade Oswaldo Cruz (FOC). He is currently a professor at the School of Health at Anhembi Morumbi University.

    Dr. Rodolfo de Paula Vieira

    Evangelical University of Goiás

    OrcID https://orcid.org/0000-0001-6379-1143

    Graduated in Physical Education (Univap 1998-2001), Master's degree in Biological Sciences (Univap 2001-2003), Doctorate in Pathology (CAPES Scholarship, Faculty of Medicine USP 2004-2007) and Post-doctorate in Physical Activity and Pulmonary Immunopathology (FAPESP Scholarship , Faculty of Medicine USP 2008-2009), Post-doctorate in Asthma Immunology (Deutsche Forchungsgemeinschaft Contract 2009-2010) from the Albert-Ludwigs University of Freiburg - Germany, Post-doctorate in Physical Activity and Pulmonary Immunopathology (European Respiratory Award Scholarship Society/Marie Curie Foundation/European Union 2010-2011). He was a guest researcher at the Department of Pulmonology at the Albert-Ludwigs University of Freiburg in Germany (June 2010 to May 2011) and an associate researcher in the same department (June 2011 to December 2011). He is the researcher responsible for the project "Effects of aerobic physical training on lung changes induced by different products of atmospheric pollution" financed by MCT / CNPq, which is part of the (INCTs-National Institutes of Science and Technology). Since April/2014 he has been the author and responsible for the Young Researcher Project FAPESP 2012/15165-2 entitled "Role of purinergic signaling and SOCS-JAK-STAT signaling in the anti-inflammatory effects of aerobic training in experimental models of asthma and in asthmatic individuals" , from where he obtained resources to set up the Pulmonary and Exercise Immunology Laboratory (LABPEI) in the city of São José dos Campos. He is currently director of the Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE). He is coordinator of LABPEI (www.labpei.com.br), which is the functional arm of IBEPIPE, developing clinical and experimental research work, focusing on cellular and molecular immunological aspects involved in both the pathophysiology of lung diseases and the effects of physical exercise in lung diseases. Furthermore, the laboratory's research focus includes research in the area of exercise immunology, which is carried out with professional and amateur athletes and different sports teams. Advisor of the master's and doctorate programs in Human Movement Sciences and Rehabilitation at UNIFESP (and collaborating professor) and the master's and doctorate programs in Human Movement and Rehabilitation at Unievangélica and also the professional master's degree in Pharmaceutical Sciences at Unievangélica. He has been a CNPq level 2 research productivity fellow since 2016. Web of Science ResearcherID: E-9315-2011.

    Drª Marília Cristina Duarte

    University of Mogi das Cruzes

    OrcID https://orcid.org/0000-0002-1257-3389

    PhD and master's degree in Plant Biodiversity and the Environment, in the area of plant systematics, from the São Paulo Botany Institute. Currently, she is a researcher and professor at undergraduate and postgraduate levels at the University of Mogi das Cruzes (UMC), as well as curator of the Herbarium Mogiense (HUMC). She has experience in the area of Botany, with an emphasis on Spermatophyte Systematics, especially from the Malvaceae family, working mainly on floristic inventories and institutional projects. She has published scientific articles, book chapters and participated in National and International Botany Congresses.

Keywords

Cymbopogon martini
Anti-inflammatory
Anti-aging
Hyaluronic acid
Collagen

Abstract

The aim of the study was to evaluate the effects of essential oil from Cymbopogon martinii (CMEO) on lipopolysaccharide (LPS)-stimulated human fibroblast cells. The fibroblasts were cultured using Iscove's medium and stimulated by LPS (1μg/mL). The cytotoxicity of CMEO was evaluated by MTT and collagen concentration by Sirius red. Collagenase activity, hyaluronic acid, and the concentrations of IL-1β; IL-6; MCP-1, and MIP-1-α were evaluated by ELISA. The effect of CMEO on the mRNA levels and the secretion of MMP-1; MMP-2; and MMP-9 enzymes in fibroblasts were evaluated by RT-qPCR and ELISA, respectively. CMEO was cytotoxic against fibroblasts, in which 10μg/mL inhibited 50% of cell viability. When treated with CMEO, the fibroblasts produced more collagen and hyaluronic acid compared to control cells. When stimulated by LPS, fibroblasts exhibited higher production of IL-6, IL-1β, MCP-1, and MIP-1α compared to control cells. However, the treatment of fibroblasts with CMEO reduces cytokines secretion and enzyme expression. In summary, CMEO reduced the secretion of cytokines and metalloproteinases, in addition to increasing the synthesis of extracellular matrix elements. The study demonstrated the effects of CMEO on the modulation of inflammatory mediators and decreasing the mRNA and secretion levels of metalloproteinases.

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Brazilian essential oil of Cymbopogon martinii: positive effects on inflammation-induced human fibroblasts and skin aging. Rev Fitos [Internet]. 2024 Jun. 6 [cited 2025 Jan. 23];18:e1130. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1130
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