Biochemical and morphological parameters in diabetic rats treated with extracts of Ora-Pro-Nóbis (Pereskia aculeata)

Mr. João Paulo Lopes Jordão
OrcID
Mr. Gabriel Lopes Chaves
OrcID
Mr. Guilherme Fantinato
OrcID
Ms. Kamila Oliveira de Luca Rotondo
OrcID
Ms. Michele Caroline Terra
OrcID
Ms. Sofia de Castro Oliveira
OrcID
Aluísio Eustáquio de Freitas Miranda Filho
OrcID
Dr.ª Ana Claudia Frota Lopes
OrcID
Dr. Roberta Bessa Veloso Silva
OrcID
Dr. Bruno Cesar Correa Salles
OrcID
Dr. Cláudio Daniel Cerdeira
OrcID
Me. Gérsika Bitencourt Santos Barros
OrcID

    Mr. João Paulo Lopes Jordão

    José do Rosario Vellano University (UNIFENAS), Highway 179, Km 0, s/n, Cloverleaf, Zip Code: 37130-000, Alfenas, MG, Brazil.

    OrcID https://orcid.org/0000-0001-7596-1031

    Seventh-semester medical student at José do Rosário Vellano University (Unifenas - Alfenas/MG). Member of the UNIVIDA First Aid League and member of the board of the Academic League of Cardiac and Thoracic Surgery (LACCT), as Scientific Coordinator, and of the League of Morphological Sciences (LICIEM), as Secretary. Paid monitor in the Anatomy I discipline (2019), voluntary monitor in the Anatomy III discipline (2020) and in the disciplines of Urgency and Emergency I and II (2020/2021), all through the Medical Course. Scientific Initiation Student through the PROBIC/UNIFENAS program (2020/2021).

    Mr. Gabriel Lopes Chaves

    José do Rosario Vellano University (UNIFENAS), Highway 179, Km 0, s/n, Cloverleaf, Zip Code: 37130-000, Alfenas, MG, Brazil.

    OrcID https://orcid.org/0000-0001-8735-8344

    He has a high school diploma from Colégio Espirita Professor Rubens Costa Romanelli (2014). He has experience in the area of ​​Geography.

    Mr. Guilherme Fantinato

    José do Rosario Vellano University (UNIFENAS), Highway 179, Km 0, s/n, Cloverleaf, Zip Code 37130-000, Alfenas, MG, Brazil.

    OrcID https://orcid.org/0000-0002-8968-7093

    Sixth-year student of the Medicine course at José do Rosário Vellano University (Unifenas - Alfenas/MG). President of the Sports Medicine League (LIAME), member of the Angiology League (LACV). Monitor in the Pharmacology 2 discipline for the Medicine course.

    Ms. Kamila Oliveira de Luca Rotondo

    Universidade José do Rosário Vellano (UNIFENAS), Rodovia 179, Km 0, s/n, Trevo, CEP: 37130-000, Alfenas, MG, Brasil.

    OrcID https://orcid.org/0000-0002-7808-0755

    Graduated in Biomedicine from José do Rosário Vellano University - UNIFENAS - Alfenas/MG. I participated as a collaborator in the scientific initiation project: "Evaluation of the effects of Artichoke extract (CYNARA SCOLUMUS L.) on biochemical parameters in diabetic rats", which generated an article already published in the International Journal of Development Research. I had experience as a monitor in two subjects: Organic Chemistry and Physiology. I participated in the testing of students against COVID-19, for their return to classes. I carried out my final course work experimentally, in which we worked with diabetic animals, generating an article: "EFFECT OF ORA-PRO-NÓBIS EXTRACTS (Pereskia aculeata) ON BIOCHEMICAL PARAMETERS AND OXIDATIVE STRESS IN DIABETIC RATS", submitted for approval in the Fitos journal, from Fiocruz. I completed an internship at the Clinical Analysis laboratory at Santa Casa de Alfenas-MG.

    Ms. Michele Caroline Terra

    José do Rosario Vellano University (UNIFENAS), Highway 179, Km 0, s/n, Cloverleaf, Zip Code: 37130-000, Alfenas, MG, Brazil.

    OrcID https://orcid.org/0000-0003-4139-4256

    Graduated in Biomedicine from the José do Rosário Vellano University, Alfenas/MG. Currently, a master's student in the Materials Science and Technology program with an emphasis on the development of nanoparticle formulations containing essential oils for the control of pests of agricultural interest, at the São Paulo State University Júlio de Mesquita Filho - Unesp, Sorocaba/SP. Knowledge in the areas of clinical biochemistry, clinical hematology and characterization of natural products through scientific initiation with animal experimentation in diabetic rats and their complementary treatments using products of plant origin. Has experience in the Hematology sector as an Analyst responsible for the Immunohematology, glycated hemoglobin and coagulation sector.

    Ms. Sofia de Castro Oliveira

    José do Rosario Vellano University (UNIFENAS), Highway 179, Km 0, s/n, Cloverleaf, Zip Code: 37130-000, Alfenas, MG, Brazil.

    OrcID https://orcid.org/0000-0002-1863-2079

    Graduated in Biomedicine from the José do Rosário Vellano University - Alfenas/MG. Knowledge in the areas of Clinical Biochemistry, Clinical Hematology and characterization of natural products, through experience in scientific research through animal experimentation with diabetes mellitus and complementary treatments using products of plant origin. In addition to experience in clinical analysis by the laboratory of the Santa Casa de Alfenas Hospital.

    Aluísio Eustáquio de Freitas Miranda Filho

    José do Rosario Vellano University (UNIFENAS), Highway 179, Km 0, s/n, Cloverleaf, Zip Code: 37130-000, Alfenas, MG, Brazil.

    OrcID https://orcid.org/0000-0002-1348-322X

    Graduated in Biomedicine from the José do Rosário Vellano University - Alfenas/MG. Knowledge in the areas of Clinical Biochemistry, Clinical Hematology and characterization of natural products, through experience in scientific research through animal experimentation with diabetes mellitus and complementary treatments using products of plant origin. In addition to experience in clinical analysis by the laboratory of the Santa Casa de Alfenas Hospital.

    Dr.ª Ana Claudia Frota Lopes

    University of Sao Paulo (USP), Dr. Arnaldo Avenue 455, Zip Code 01246-903, Sao Paulo, SP, Brazil.

    OrcID https://orcid.org/0000-0002-3274-9636

    Graduated in Medicine from the State University of Montes Claros (2012). Medical residency in Pathological Anatomy at the Hospital das Clínicas of the University of São Paulo (USP-SP 2016). PhD in Sciences from the School of Medicine of the University of São Paulo (USP-SP). She completed an observership in Hematopathology at the MD Anderson Cancer Center of the University of Texas at Houston/USA. Full member of the Brazilian Society of Pathology. She was a professor of Pathology at the FIPMoc University Center (UniFIPMoc - Montes Claros), pathologist at the Clemente Faria Hospital of the State University of Montes Claros (HUCF-Unimontes) and pathologist at the Citomed Laboratory. She is currently a resident physician in Pathological Anatomy and Clinical Pathology at Houston Methodist.

    Dr. Roberta Bessa Veloso Silva

    José do Rosario Vellano University (UNIFENAS), Faculty of Medicine, Highway 179, Km 0, s/n, Cloverleaf, Zip Code: 37130-000, Alfenas, MG, Brazil

    OrcID https://orcid.org/0000-0003-4794-5872

    Graduated in Business Administration from the Federal University of Lavras (UFLA, 2002), with a master's degree (2005) and doctorate (2009) in Statistics and Agricultural Experimentation (UFLA). She has extensive experience in higher education: several undergraduate courses in the agronomic and health areas, postgraduate degree in Production Systems in Agriculture, postgraduate degree in Animal Science, postgraduate degree in Animal Reproduction and MBA in Administration (UNIFENAS Alfenas campus, Varginha campus and Alfenas Teaching and Research Foundation - FETA). She has experience in consulting on planning and analyzing experiments, using R software, and Multivariate Statistics. In the first semester of 2024, she worked as Administrator of the Sigmae Journal of the Department of Exact Sciences of the Federal University of Alfenas, Alfenas campus, acting as editor. She has experience both with the publication of scientific articles and with the use of the OJS Platform.

    Dr. Bruno Cesar Correa Salles

    José do Rosario Vellano University (UNIFENAS), Highway 179, Km 0, s/n, Cloverleaf, Zip Code: 37130-000, Alfenas, MG, Brazil

    OrcID https://orcid.org/0000-0002-4444-9673

    He holds a degree in Biomedicine from the Federal University of Alfenas (2011), a Master's and a PhD in Pharmaceutical Sciences from the Federal University of Alfenas (2014/2017). He has experience in the area of ​​Biomedicine, teaching the subjects of Biochemistry, Clinical Biochemistry, Immunology and Clinical Hematology, working mainly in the use of natural products in the complementary treatment of Diabetes mellitus aimed at preventing the onset of its chronic cardiovascular complications. I was Coordinator of the Biomedicine Course at UNIFENAS. He is currently Director of Research and Postgraduate Studies, Professor of Higher Education and is part of the Structuring Teaching Nucleus of the Biomedicine course at Professor Edson Antônio Velano University/Alfenas Teaching and Technology Foundation (UNIFENAS/FETA).

    Dr. Cláudio Daniel Cerdeira

    Independent Researcher, Brazil.

    OrcID https://orcid.org/0000-0002-7242-8028

    Cláudio Daniel Cerdeira, B.el. (Biomedicine), M.e. (Pharmaceutical Sciences) and D.r. (Pharmaceutical Sciences) is a Brazilian Professor, Researcher and Scientist with a considerable productive contribution in the health area. Currently and during his master's and doctoral studies he has been working with the involvement of oxidative/nitrosative stress in the pathophysiology of inflammatory-infectious diseases and non-communicable diseases (Diabetes Mellitus, dyslipidemia and cardiovascular diseases). He has studied the participation of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex, generating superoxide and subsequently other reactive oxygen (ROS) and nitrogen (RNS) species, in the genesis and/or progression of these diseases. Furthermore, his research interests extend to areas addressing the involvement of oxidative and nitrosative stresses/ROS balance in cell signaling (including METs/NETs activation), the involvement of protein disulfide isomerase (PDI) and the influence of laser therapy/photobiomodulation (low-level laser), antioxidants and other compounds on the immune response of phagocytes against microorganisms of medical relevance (mainly Staphylococcus aureus, Candida albicans and Mycobacterium tuberculosis). During the last years, he has been working with the evaluation of antimicrobial, cytotoxic and antioxidant activities of natural compounds and with the global public health problem involving antimicrobial resistance.

    Me. Gérsika Bitencourt Santos Barros

    José do Rosario Vellano University (UNIFENAS), Faculty of Medicine, Highway 179, Km 0, s/n, Cloverleaf, Zip Code: 37130-000, Alfenas, MG, Brazil

    OrcID https://orcid.org/0000-0003-0849-2786

    Professor of Pharmacology and Cellular and Molecular Basis at Prof. Edson Antônio Velano University, Medicine, Pharmacy, Nursing, Nutrition, Dentistry, Psychology courses and in the Specialization in Urgency and Emergency and Specialization in ICU courses. Member of the Pharmacy and Therapeutics Commission (CFT) of Alfenas. Coordinator of the Pharmaceutical Care Leagues (LAF) and Pharmacology and Therapeutics (LAFT). Teaching representative of the Pharmacy and Medicine course boards. Graduated in Pharmacy from Prof. Edson Antônio Velano University. Master's and PhD in Pharmaceutical Sciences from the Federal University of Alfenas (UNIFAL-MG). Experience in oxidative stress and biochemical tests for anti-inflammatory and antioxidant evaluation of compounds, in addition to having as a line of research the study of substances with antidiabetic potential in animal models.

Keywords

Pereskia aculeata
Diabetes mellitus
AST
Urea
Oxidative stress

Abstract

Diabetes mellitus (DM) is a serious global public health problem. DM complications corroborate with high morbidity and mortality rates. Thus, several plant species have been studied regarding their medicinal properties with potential use in the treatment of DM and/or its complications. This study evaluated the effects of leaf (LE-OPN) and bark (BE-OPN) extracts from ora-pro-nóbis (Pereskia aculeata) on biochemical parameters (glycemia, triglycerides, total cholesterol, creatine, urea, AST and ALT, and lipid peroxidation) and histology (heart, liver and kidneys) in animals with DM. Induction of type 1 DM (glycemia > 200 mg/dl) in male Wistar rats was performed with Alloxane (150 mg/kg/ml). The animals were divided into 4 groups: (1) without DM, (2) with DM, (3) with DM treated with LE-OPN and (4) with DM treated with BE-OPN. The treatment was done by gavage (300 mg/kg/day) for 4 weeks. After this period the parameters were evaluated. BE-OPN prevented the increase in triglycerides, AST, ALT, and urea, in addition to lipid peroxidation (p < 0.05). The results suggest a potential for BE-OPN to prevent typical complications of DM, improving the lipid profile, liver, and kidney, as well as attenuating oxidative stress.

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Biochemical and morphological parameters in diabetic rats treated with extracts of Ora-Pro-Nóbis (Pereskia aculeata). Rev Fitos [Internet]. 2024 Oct. 2 [cited 2024 Nov. 21];18. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1328
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