Brasil
 
O conteúdo desse portal pode ser acessível em Libras usando o VLibras

Antifungal activity of Garcinia gardneriana (Planch. & Triana) Zappi leaves against Cryptococcus neoformans and Cryptococcus gattii

    Teacher Arthur Borborema Costa

    Federal University of Juiz de Fora (UFJF), Governador Valadares Advanced Campus, Rua Manoel Byrro, 241, Vila Bretas, CEP 35032-620, Governador Valadares, MG, Brazil.

    OrcID https://orcid.org/0000-0001-9545-1688

    Farmacéutico Generalista con énfasis en análisis clínicos. Miembro del grupo de investigación registrado en el CNPq titulado: Investigación y Desarrollo de Moléculas Bioactivas de Origen Natural y Sintético. Tiene experiencia en pruebas de laboratorio en las áreas de parasitología, microbiología, bioquímica y hematología. Experiencia también en dispensación de medicamentos controlados, control de procesos en farmacias dispensadoras, entre otros. Licenciatura en Farmacia por la Universidad Federal de Juiz de Fora (UFJF) - Campus Avanzado Governador Valadares - MG (SET/2022).

    Teacher Daniel Victor Montes de Melo

    Federal University of Juiz de Fora (UFJF), Governador Valadares Advanced Campus, Rua Manoel Byrro, 241, Vila Bretas, CEP 35032-620, Governador Valadares, MG, Brazil.

    OrcID https://orcid.org/0000-0002-0790-928X

    Graduating from the 9th period of the pharmacy course at the Federal University of Juiz de Fora Campus Governador Valadares. He has experience as a monitor in the subjects General Chemistry and Pharmaceutical Chemistry in the Department of Pharmacy at the Federal University of Juiz de Fora Campus Governador Valadares. He participated as a member of the Academic League of Microbiology and Infectious Diseases at the Federal University of Juiz de Fora Campus Governador Valadares and is co-founder of the Academic League for Medication Therapy Management at the Federal University of Juiz de Fora Campus Governador Valadares. During his undergraduate studies he participated in scientific initiation projects focusing on medicinal plants and natural products.

    Master Queila da Silva Rosa

    Federal University of Minas Gerais (UFMG), Institute of Biological Sciences, Department of Microbiology, Mycology Laboratory. Av. Presidente Antônio Carlos, 6627, H3, H310, Pampulha, CEP 31270-901, Belo Horizonte, MG, Brazil.

    OrcID https://orcid.org/0000-0003-3985-5607

    PhD student in Microbiology at the Federal University of Minas Gerais. Master in Biochemistry and Molecular Biology from the Federal University of Juiz de Fora - Multicenter Program in Biochemistry and Molecular Biology. Graduated in Nutrition from the Federal University of Juiz de Fora (2017).

    Teacher Mônica Maria de Almeida

    Federal University of Juiz de Fora (UFJF), Governador Valadares Advanced Campus. Rua Manoel Byrro, 241, Vila Bretas, CEP 35032-620, Governador Valadares, MG, Brazil; Universidade Vale do Rio Doce (UNIVALE), Faculty of Health Sciences. Rua Israel Pinheiro, 2000, Block F14 Universitário, CEP 35000-000, Governador Valadares, MG, Brazil.

    OrcID https://orcid.org/0000-0001-8846-1837

    She has a degree in Biological Sciences from Universidade Vale do Rio Doce/UNIVALE (2006), a Master's degree in Immunopathology of Infectious and Parasitic Diseases (2009) and is a PhD student in Biochemistry and Molecular Biology of the Multicenter Postgraduate Program at the Federal University of Juiz de Fora. de Fora Governador Valadares campus. She has experience in the areas of Immunology, Parasitology and Microbiology, with an emphasis on Mycology. She is a member of the Structuring Teaching Center and works as a professor in the Medicine course in the Morphophysiochemistry I and Infectious and Parasitic Diseases I and II modules. Since 2018, she has been responsible for Coordinating Curricular and Institutional Extension, Monitoring and Complementary Medicine Activities / UNIVALE. She coordinates Research Projects in the area of antimicrobials (antifungals) and participates, in collaboration, in Projects in the area of bacterial resistance in a hospital environment.

    Mrs. Beatriz Joia Tabai

    Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Mucuri Campus, Faculty of Medicine - FAMMUC. Rua Cruzeiro, 01, Jardim São Paulo, CEP 39803-371, Teófilo Otoni, MG, Brazil.

    OrcID https://orcid.org/0000-0001-7011-5792

    Medical student at the Federal University of Vales do Jequitinhonha and Mucuri, Campus Mucuri.

    Dr. Caio César de Souza Alves

    Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Mucuri Campus, Faculty of Medicine - FAMMUC. Rua Cruzeiro, 01, Jardim São Paulo, CEP 39803-371, Teófilo Otoni, MG, Brazil.

    OrcID https://orcid.org/0000-0001-9765-8527

    He has a degree in Biological Sciences from the Federal University of Juiz de Fora (2004), a master's degree in Brazilian Health from the Federal University of Juiz de Fora (2007) and a doctorate in Health from the Federal University of Juiz de Fora (2012). She was a fellow of the PhD Program with Internship Abroad-PDEE-in the period 2010-2011, at the University of Newcastle-Australia. He was a Post-Doctoral fellow (2013). He is currently an adjunct professor at the Federal University of Vales do Jequitinhonha and Mucuri-Campus Mucuri. He has experience in the area of Immunology, with an emphasis on Immunopharmaceuticals, working mainly on the following topics: nitric oxide, pulmonary allergy, EAE, cell culture, flow cytometry and ELISA.

    Profª Sandra Bertelli Ribeiro de Castro

    Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Mucuri Campus, Faculty of Medicine - FAMMUC. Rua Cruzeiro, 01, Jardim São Paulo, CEP 39803-371, Teófilo Otoni, MG, Brazil.

    OrcID https://orcid.org/0000-0002-5535-0919

    Adjunct Professor at the Faculty of Medicine of Mucuri (FAMMUC) - Federal University of Vales do Jequitinhonha and Mucuri - UFVJM, Post-Doctorate from the National Post-Doctoral Program (PNPD), in the Department of Parasitology, Microbiology and Immunology of the Federal University of Juiz de Away (UFJF). PhD in Health from the postgraduate program in Health at the Federal University of Juiz de Fora (UFJF-MG). Master in Agricultural Microbiology from the Federal University of Lavras (UFLA-MG). Graduated in Pharmacy and Biochemistry from UFJF with a specialization in Quality Control and Assurance from Centro Universitário Newton Paiva. Professor of the Postgraduate Program in Health Sciences (PPGCS- UFVJM). Professor in the Multicenter Postgraduate Program in the area of Biochemistry and Molecular Biology (PMBqBM) of the Brazilian Society of Biochemistry and Molecular Biology (UFJF-GV), with experience in the culture of tumor and non-tumor cells, development of an experimental model of edema of acute and chronic disease and Experimental Autoimmune Encephalomyelitis, coordinates projects related to the evaluation of the biological activity of new substances and repositioning of drugs.

    Dr. Karen Luise Lang

    Federal University of Juiz de Fora (UFJF), Governador Valadares Advanced Campus. Rua Manoel Byrro, 241, Vila Bretas, CEP 35032-620, Governador Valadares, MG, Brazil.

    OrcID https://orcid.org/0000-0002-1441-6280

    Professor of Pharmaceutical Chemistry at the Federal University of Juiz de Fora - Campus Governador Valadares, MG. Graduated in Pharmacy from the Federal University of Santa Catarina (2005). Master in Pharmacy (Drugs and Medicines) from the Federal University of Santa Catarina (2006) and PhD in Chemistry (Organic Chemistry) from the same institution, in 2012, with a sandwich period at the University of Buenos Aires (UBA, Buenos Aires, Argentina). She completed a post-doctoral internship at the UFSC Pharmaceutical Chemistry Laboratory (2012-2013). She has experience in the field of Pharmacy, with an emphasis on Chemistry of Natural Products, working on projects in the area of chemical research and obtaining synthetic derivatives of natural products, targeting biologically active compounds.

    Drª Gabriella Freitas Ferreira

    Federal University of Juiz de Fora (UFJF), Governador Valadares Advanced Campus. Rua Manoel Byrro, 241, Vila Bretas, CEP 35032-620, Governador Valadares, MG, Brazil.

    OrcID https://orcid.org/0000-0001-6842-1934

    He has a degree in Pharmacy from Universidade Vale do Rio Doce (2006), a master's degree in Biological Sciences (Immunopathology of infectious-parasitic diseases) from Universidade Vale do Rio Doce (2009) and a PhD in Biological Sciences (Microbiology) from the Federal University of Minas Gerais ( 2015). She is currently a professor at the Federal University of Juiz de Fora-Campus Governador Valadares. She has experience in the area of Microbiology, with an emphasis on Medical Microbiology, working mainly in the area of antimicrobials.

Keywords

Abstract

Cryptococcosis is a fungal infection that can lead to serious illnesses such as pneumonia and meningoencephalitis. Currently, there are few therapeutic options for the treatment of systemic fungal infections and drug resistance is increasing. In this regard, natural products are a source of lead compounds in drug discovery research. This study aimed to investigate the anticryptococcal activity of Garcinia gardneriana in vitro. We obtained the ethanolic extract, fractions, epicuticular wax, and triterpene lupeol From the leaves. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were evaluated against twelve strains of Cryptococcus neoformans and Cryptococcus gattii. The hexane fraction (FHGG) showed the best antifungal activity. FHGG and lupeol (isolated from FHGG) showed MIC and MFC values between 12.5 to 100 µg/mL and 25 to 100 µg/mL, respectively. We determined the cytotoxicity against murine macrophage cells. Lupeol has a selectivity index value greater than 1. Thus, we suppose the lupeol is a candidate for further studies since it exhibited significant anticryptococcal activity.

References

  1. Kwon-Chung KJ, Fraser JA, Doering TL, Wang Z, Janbon G, Idnurm A et al. Cryptococcus neoformans and Cryptococcus gattii, the etiologic agents of cryptococcosis. Cold Spring Harb Perspect Med [online]. 2014; 4(7): a019760. [acesso em: 23 mai. 2020]. Disponível em: [https://doi.org/10.1101/cshperspect.a019760].
  2. Maziarz EK, Perfect JR. Cryptococcosis. Infect Dis Clin North Am [online]. 2016 mar; 30(1): 179-206. [acesso em: 27 mai. 2020]. Disponível em: [https://doi.org/10.1016/j.idc.2015.10.006] [https://pubmed.ncbi.nlm.nih.gov/26897067/].
  3. Spadari CC, Wirth F, Lopes LB, Ishida K. New Approaches for Cryptococcosis Treatment. Microorganisms [online]. 2020; 8(4): 613. [acesso em: 4 mai. 2020]. Disponível em: [https://doi.org/10.3390/microorganisms8040613].
  4. Rajasingham R, Smith RM, Park BJ, Jarvis JN, Govender NP, Chiller TM et al. Global burden of disease of HIV-associated cryptococcal meningitis: an updated analysis. Lancet Infect Dis [online]. 2017 aug; 17(8): 873-881. [acesso em: 2 mai. 2020]. Disponível em: [https://doi.org/10.1016/S1473-3099(17)30243-8] [https://pubmed.ncbi.nlm.nih.gov/28483415/].
  5. Soares EA, Lazera, S, Wanke, B, Ferreira F, Oliveira AG, Coutinho ZF. Mortality by cryptococcosis in Brazil from 2000 to 2012: a descriptive epidemiological study. PLOS Negl Trop Dis [online]. 2019; 13(7): [acesso em: 2 mai. 2020]. Disponível em: [https://doi.org/10.1371/journal.pntd.0007569] [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6687200/].
  6. Coutinho ZF, Wanke B, Travassos C, Oliveira RM, Xavier DR, Jr. Coimbra CE. Hospital morbidity due to paracoccidioidomycosis in Brazil (1998-2006). Trop Med Int Health [online]. 2015; May; 20(5): 673-680. [acesso em: 2 mai. 2020]. Disponível em: [https://doi.org/10.1111/tmi.12472] [https://pesquisa.bvsalud.org/portal/resource/pt/lil-772822].
  7. Brasil, Ministério da Saúde. Banco de dados do Sistema Único de Saúde. Fonte: MS/SVS/CGIAE - Sistema de Informações sobre Mortalidade – SIM. DATASUS2020. [acesso em: 21 mar. 2022] Disponível em: [http://tabnet.datasus.gov.br/cgi/tabcgi.exe?sim/cnv/obt10br.de].
  8. Singulani JL, Galeane MC, Ramos MD, Gomes PC, Dos Santos CT, de Souza BM et al. Antifungal Activity, Toxicity, and Membranolytic Action of a Mastoparan Analog Peptide. Front Cell Infect Microbiol [online]. 2019; Dec 6; 9: 419. [acesso em: 02 set. 2021]. Disponível em: [https://doi.org/10.3389/fcimb.2019.00419] [https://pubmed.ncbi.nlm.nih.gov/31867293/].
  9. Newman DJ, Cragg GM. Natural Products as Sources of New Drugs over the Nearly Four Decades from 01/1981 to 09/2019. J Nat Prod [online]. 2020 Mar 27; 83(3): 770-803. [acesso em: 14 ago. 2022]. Disponível em: [https://pubs.acs.org/doi/pdf/10.1021/acs.jnatprod.9b01285].
  10. Espírito Santo BLSD, Santana LF, Kato Junior WH, de Araújo FO, Bogo D, Freitas KC et al. Medicinal Potential of Garcinia Species and Their Compounds. Molecules [online]. 2020; Oct. 1; 25(19): 4513. [acesso em: 01 set. 2021]. Disponível em: [https://doi.org/10.3390/molecules25194513] [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7582350/].
  11. Rocha AP, Matos VP, Sena LHM, Pacheco MV, Ferreira RLC. Métodos para superação da dormência em sementes de Garcinia gardneriana (Planch. & Triana) Zappi. Ciênc Florest [online]. 2018; 28: 510-514. [acesso em: 01 set. 2021]. [https://doi.org/10.5902/1980509832031].
  12. Ramadwa TE, McGaw LJ, Adamu M, Madikizela B, Eloff JN. Anthelmintic, antimycobacterial, antifungal, larvicidal and cytotoxic activities of acetone leaf extracts, fractions and isolated compounds from Ptaeroxylon obliquum (Rutaceae). J Ethnopharmacol [online]. 2021 Nov 15; 280: 114365. [acesso em: 15 ago. 2022]. Disponível em: [https://doi.org/10.1016/j.jep.2021.114365] [https://pubmed.ncbi.nlm.nih.gov/34175445/].
  13. Shahlaei M, Ghanadian SM, Ayatollahi AM, Mesaik, MA, Abdalla, OM, Afsharypour S et al. Molecular modeling, structure activity relationship and immunomodulatory properties of some lupeol derivatives. Med Chem Res [online]. 2013; 22: 1795-1803. [acesso em: 15 ago. 2022]. Disponível em: [https://doi.org/10.1007/s00044-012-0183-y].
  14. Clinical and Laboratory Standards Institute (CLSI). Reference method for broth dilution antifungal susceptibility testing of yeasts. Approved standard M27-A3, 3rd ed. Clinical and Laboratory Standards Institute, Wayne, PA, 2008. Disponível em: [https://clsi.org/].
  15. Abedini A, Colin M, Hubert J, Charpentier E, Angelis A, Bounasri H et al. Abundant Extractable Metabolites from Temperate Tree Barks: The Specific Antimicrobial Activity of Prunus Avium Extracts. Antibiotics (Basel). 2020 Mar 4; 9(3): 111. Disponível em: [https://doi.org/10.3390/antibiotics9030111] [https://pubmed.ncbi.nlm.nih.gov/32143394/].
  16. Folly MLC, Ferreira GF, Salvador MR, Sathler AA, da Silva GF, Santos JCB et al. Evaluation of in vitro Antifungal Activity of Xylosma prockia (Turcz.) Turcz. (Salicaceae) leaves against Cryptococcus spp. Front Microbiol [online]. 2020 Feb 6; 10: 3114. [acesso em: 15 ago. 2022]. Disponível em: [https://doi.org/10.3389/fmicb.2019.03114] [https://pubmed.ncbi.nlm.nih.gov/32117083/].
  17. Barbosa Junior AM, Melo DLFM, Almeida FTC, Trindade RC. Estudo comparativo da susceptibilidade de isolados clínicos de Cryptococcus neoformans (Sanfelice, 1895) frente a alguns antifúngicos de uso hospitalar e extratos vegetais obtidos de plantas medicinais da região semiárida sergipana. Rev Bras Pl Med [online]. 2015; 17(1): 120-132. [acesso em: 6 fev. 2021]. Disponível em: [https://doi.org/10.1590/1983-084X/11_177].
  18. Javed S, Mahmood Z, Khan, KM, Sarker S, Jarvaid A et al. Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina. Sci Rep [online]. 2021; 11: 8417. [acesso em: 6 jan. 2022]. Disponível em: [https://doi.org/10.1038/s41598-021-87725-7].
  19. Ríos JL, Recio MC. Medicinal plants and antimicrobial activity. J Ethnopharmacol [online]. 2005; 100(1-2): 80-84. [acesso em: 6 jan. 2022]. Disponível em: [https://doi.org/10.1016/j.jep.2005.04.025] [https://pubmed.ncbi.nlm.nih.gov/15964727/].
  20. Silva PR, Rabelo RAS, Terra APS, Teixeira DNS. Susceptibilidade a antifúngicos de variedades de Cryptococcus neoformans isoladas de pacientes em hospital universitário. Rev Soc Bras Med Trop [online]. 2008; 41(2): 158-162. [acesso em: 8 jan. 2022]. Disponível em: [https://doi.org/10.1590/S0037-86822008000200005].
  21. Hafidh RR, Abdulamir AS, Vern LS, Abu Bakar F, Abas F, Jahanshiri F et al. Inhibition of growth of highly resistant bacterial and fungal pathogens by a natural product. Open Microbiol J [online]. 2011; 5: 96-106. [acesso em: 25 nov. 2021]. Disponível em: [https://doi.org/10.2174/1874285801105010096] [https://pubmed.ncbi.nlm.nih.gov/21915230].
  22. Gucwa K, Milewski S, Dymerski T, Szweda P. Investigation of the Antifungal Activity and Mode of Action of Thymus vulgaris, Citrus limonum, Pelargonium graveolens, Cinnamomum cassia, Ocimum basilicum, and Eugenia caryophyllus Essential Oils. Molecules. 2018 May 8; 23(5): 1116. Disponível em: [https://doi.org/10.3390/molecules23051116] [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099571/].
PDF (Portuguese)
HTML (Portuguese)
XML (Portuguese)
Save bookmark

Author(s)

  • Teacher Arthur Borborema Costa
    Federal University of Juiz de Fora (UFJF), Governador Valadares Advanced Campus, Rua Manoel Byrro, 241, Vila Bretas, CEP 35032-620, Governador Valadares, MG, Brazil.
    https://orcid.org/0000-0001-9545-1688
  • Teacher Daniel Victor Montes de Melo
    Federal University of Juiz de Fora (UFJF), Governador Valadares Advanced Campus, Rua Manoel Byrro, 241, Vila Bretas, CEP 35032-620, Governador Valadares, MG, Brazil.
    https://orcid.org/0000-0002-0790-928X
  • Master Queila da Silva Rosa
    Federal University of Minas Gerais (UFMG), Institute of Biological Sciences, Department of Microbiology, Mycology Laboratory. Av. Presidente Antônio Carlos, 6627, H3, H310, Pampulha, CEP 31270-901, Belo Horizonte, MG, Brazil.
    https://orcid.org/0000-0003-3985-5607
  • Teacher Mônica Maria de Almeida
    Federal University of Juiz de Fora (UFJF), Governador Valadares Advanced Campus. Rua Manoel Byrro, 241, Vila Bretas, CEP 35032-620, Governador Valadares, MG, Brazil; Universidade Vale do Rio Doce (UNIVALE), Faculty of Health Sciences. Rua Israel Pinheiro, 2000, Block F14 Universitário, CEP 35000-000, Governador Valadares, MG, Brazil.
    https://orcid.org/0000-0001-8846-1837
  • Mrs. Beatriz Joia Tabai
    Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Mucuri Campus, Faculty of Medicine - FAMMUC. Rua Cruzeiro, 01, Jardim São Paulo, CEP 39803-371, Teófilo Otoni, MG, Brazil.
    https://orcid.org/0000-0001-7011-5792
  • Dr. Caio César de Souza Alves
    Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Mucuri Campus, Faculty of Medicine - FAMMUC. Rua Cruzeiro, 01, Jardim São Paulo, CEP 39803-371, Teófilo Otoni, MG, Brazil.
    https://orcid.org/0000-0001-9765-8527
  • Profª Sandra Bertelli Ribeiro de Castro
    Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Mucuri Campus, Faculty of Medicine - FAMMUC. Rua Cruzeiro, 01, Jardim São Paulo, CEP 39803-371, Teófilo Otoni, MG, Brazil.
    https://orcid.org/0000-0002-5535-0919
  • Dr. Karen Luise Lang
    Federal University of Juiz de Fora (UFJF), Governador Valadares Advanced Campus. Rua Manoel Byrro, 241, Vila Bretas, CEP 35032-620, Governador Valadares, MG, Brazil.
    https://orcid.org/0000-0002-1441-6280
  • Drª Gabriella Freitas Ferreira
    Federal University of Juiz de Fora (UFJF), Governador Valadares Advanced Campus. Rua Manoel Byrro, 241, Vila Bretas, CEP 35032-620, Governador Valadares, MG, Brazil.
    https://orcid.org/0000-0001-6842-1934

Metrics

  • Article viewed 103 time(s)

How to Cite

1.
Antifungal activity of Garcinia gardneriana (Planch. & Triana) Zappi leaves against Cryptococcus neoformans and Cryptococcus gattii. Rev Fitos [Internet]. 2024 Jun. 17 [cited 2025 Apr. 5];18:e1557. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1557
Crossref
Scopus
Google Scholar
Europe PMC
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2024 Revista Fitos

Report an error
Conteúdo acessível em Libras usando o VLibras Widget com opções dos Avatares Ícaro, Hosana ou Guga. Conteúdo acessível em Libras usando o VLibras Widget com opções dos Avatares Ícaro, Hosana ou Guga.