Micropropagação de Quimiotipos de Lippia Alba Mill. N. E. Br Produtores de Citral, Carvona e Linalol.

E. S. Tavares; S. G. Leitão; F. Reinert; C. L. S. Lage

doi:10.32712/2446-4775.2005.17

Micropropagation of Three Chemotypes of Lippia Alba Mill. N. E. Br. Producers of Citral, Carvone and Linalool Respectively

Vol. 1 No. 01 (2005)
67-73
04/10/2013
30/06/2005
https://doi.org/10.32712/2446-4775.2005.17

E. S. Tavares
S. G. Leitão
F. Reinert
C. L. S. Lage

Keywords

Abstract

This work describes a micropropagation protocol for three different chemotypes of Lippia alba, which essential oil’s major components are citral, carvone and linalol. L. alba is widely used in traditional medicine and among other characteristics, is of great economical interest for its essential oils. Nodal segments of three chemotypes, were cultured in vitro. Plants were grown in Murashigue and Skoog (MS) medium supplemented with sucrose and vitamins but not hormones, with and without norbixin. Plants obtained in this way were then subcultured to identify if the addition of IAA and kinetin optimized plant growth. The results showed that these hormones were not required to successfully culture and multiply the three chemotypes of L. alba in vitro. Addition of norbixin was efficient in decreasing plant tissue oxidation. This faster and more economical protocol did not affect ex vitro acclimation of plants that was also successful.

References

ADAMS, R.M.; KOENIGSBERG, S.S.; LANGHANS, R.W. In vitro propagation of Cephallotus folicularis (Australian patcher plant). Horticultural Science, v.14, n.1, p.512-513, 1979.
AGGARWAL, K.K; KHANUJA, S.P.S; AHMAD, A.; KUMAR, T.R.S; GUPTA, V.K.; KUMAR, S. Antimicrobial activity profiles of the two enantiomers of limonene and carvone isolated from the oils of Mentha spicata and Anethum sowa. Flavour and Fragrance Journal, v.17, n.1, p.59-63, 2002.
ANDRADE, M.W.; LUZ, J.M.Q.; LACERDA, A.S.; MELO, P.R.A. Micropropagação da aroeira (Myracrodrun urundeuva Fr. All). Ciências Agrotécnicas, Lavras, v.24, n.1, p.174-180, 2000.
CELIK, S.; OZYAKA, A. Effects of intraperitoneally administered lipoic acid, vitamin E and linalool on the level of total lipid and fatty acids in guinea pig brain with oxidative stress induced by H2O2. Journal of Biochemistry and Molecular Biology, v.35, n.6, p.547-552, 2002.
CHIANG, L.C.; CHIANG, W.; CHANG, M.Y.; NG, L.T.; LIN, C.C. Antileukemic activity of selected natural products in Taiwan. American Journal of Chinese Medicine, v.31, n.1, p.37-46, 2003.
CORRÊA, C.B.V. Contribuição ao estudo de Lippia alba (Mill.) N. E. Br. Ex Britt & Wilson - erva-cidreira. Revista Brasileira de Farmácia, v.73, n.3, p.57-64, 1992.
DIP, E.C.; TAVARES, E.S.; JULIÃO, L.S.; MATHEUS, M.E.; LEITÃO, S.G.; LAGE, C.L.S. Sedative properties of ethanolic extracts from Lippia alba Mill. N. E. Brown: comparison of the activities of three different chemotipes. 1o Simpósio
Brasileiro em Química Medicinal. Novas Estratégias em Planejamento racional de Fármacos. Caxambu, Minas Gerais, 2001.
ELIZABETSKY, E.; SILVA-BRUM, L. F.; SOUZA, D.O. Anticonvulsivant properties of linalool in glutamate-related seizure models. Phytomedicine, v.6, n.2, p.107-113, 1999.
ELLIS, M.D.; BAXENDALE, F.P. Toxicity of seven monoterpenoids to tracheal mites (Acari: Tarsonemidae) and their honey bee (Hymenoptera: Apidae) hosts when applied as fumigants. Journal of Economic Entomology, v.90, n.5, p.1087-1091, 1997.
FAROOQI, A.H.A.; FATIMA, S.; SHARMA, S.; KUMAR, S. Plant growth control properties of essential oils and their constituents. Journal of Medicinal and Aromatic Plants, v.22, n.1B, p.603-604, 2000.
FRIGHETTO, N.; OLIVEIRA, J.G.; SIANI, A.C.; CHAGAS, K.C. Lippia alba Mill N. E. Br. (Verbenaceae) as a source of linalool. Journal of Essential Oil Research, v.10, p.578-580, 1998.
GRATAPAGLIA, D.; MACHADO, M.A. Micropropagação In: TORRES, A.C.; STYER, L.; Buso, J.A. Cultura de tecidos e transformação genética de plantas. Brasília: Embrapa-SPI / Embrapa - CNPH, v.1, n.1, p.183-260, 1998.
GROUT, B.W.W. Photosynthesis of regenerated plantlets in vitro, and stresses of transplanting. Acta Horticulturae, v. 30, p.120-135, 1988.
GUPTA, S.K., SINGH, P.; BAJPAI, P.; RAM, G.; DIGVIJAI, S.; GUPTA, M.M.; JAIN, D.C.; KHANUJA, S.P.S., KUMAR, S. Morphogenetic variation for artemisinin and volatile oil in Artemisia annua. Industrial Crops and Products, v.16, p.2217-224, 2002.
GURGEL, V.T.; COUTO, F.E.; SANTOS, J.G.; VIANA, G.S.B. Central effects of citral, myrcene and limonene, constituents of essential oil chemotypes from Lippia alba (Mill.) N. E. Brown. Phytomedicine, v.9, n.8, p.709-714, 2002.
HEMPEL, F.D.; WELCH, D.R.; FELDMAN, L.J. Floral induction and determination: where is flowering controlled? Trends in Plant Science, v.5, n.1, p.17-21, 2000.
HUANG, L.C. Rejuvenation of trees and other perennials for restoration of plant regeneration competence. In: TORRES, A. C., CALDAS, L. S. Técnicas e aplicações da cultura de tecidos em plantas. Brasília: ABCTP/EMBRAPACNPH, p.252-264, 1990.
JULIANI JR, H.R.; KOROCH, A.R.; JULIANI, H.R.; TRIPPI, V.S. Micropropagation of Lippia junelliana (Mold.) Tronc. Plant Cell Tissue Organic Culture, v.59, p.175-179, 1999.
KOKALIS, B.N.; MARTINEZ, O.N.; RODRIGUEZ, K.R.; KLOEPPER, J.W. Development of multi-component transplant mixes for suppression of Meloidogyne ingognita on tomato (Lycopersicum esculentum). Journal of Nematology, v.34, n.4, p.362-369, 2002.
LAGE, C.L.S. Meio de cultura para a prevenção e/ou redução da oxidação de cultura de tecido. Depositante Universidade Federal do Rio de Janeiro, Pat. I. BR. 0106515-7, 2001.
LIU, C.H.; MISHRA, A.K.; HE, B.; TAN, R.X. Composition and antifungal activity of essential oils from Artemisia princeps and Cinnamomum camphora. International Pest Control, v.43, n.2, p.72-74, 2001.
MATOS, F.J.A. As ervas cidreiras do Nordeste do Brasil. Estudo de três quimiotipos de Lippia alba (Mill.) N. E. Brown (Verbenaceae) Parte II - Farmacoquímica. Revista Brasileira de Farmácia, v.77, n.4, p.137-141, 1996.
MATOS, F.J.A.; MACHADO, M.I.L.; CRAVEIRO, A.A.; ALENCAR, J.W. Essential oil composition of two chemotypes of Lippia alba grown in Northeast Brazil. Journal of Essential Oil Research v.8, p.695-698, 1996.
MELO, B.; PINTO, J.E.B.P.; LUZ, J.M.Q.; PEIXOTO, J.R.; JULIATTI, F.C. Diferentes antioxidantes no controle da oxidação, germinação e desenvolvimento das plântulas na cultura in vitro de embriões da guarirobeira. Ciências
Agrotécnicas, Lavras, v.25, n.6, p.1301-1306, 2001.
MURASHIGUE, T. & SKOOG, F. A revised medium for rapid growth and bioassays of tobacco tissue cultures. Physiologia Plantarum, v.15, p.473-479, 1962.
OKA, Y.; NACAR, S; PUTIEVSKY, E.; RAVID, U.; YANIV, Z.; SPIEGEL, Y. Nematicidal activity of essential oils and their components against the rootknot nematode. Phytopatology, v.90, n.7, p.710-715, 2000.
PEANA, A.T.; D'AQUILA, P.S.; PANIN, F.; CHESSA, M.L.; MORETTI, M.D.L.; SERRA, G.; PIPPIA, P. (-)-Linalool produces antinociception in two experimental models of pain. European Journal of Pharmacology, v.460, n.1, p.37-41, 2003.
PEANA, A.T.; D'AQUILA, P.S.; PANIN, F.; SERRA, G.; MORETTI, M.D.L. Antiinflammatory activity of linalool and linalyl acetate constituents of essential oil. Phytomedicine, v.9, n.8, p.721-726, 2002.
RETAMAR, J.A. Variaciones fitoquimicas de la especie Lippia alba (salvia morada) y sus aplicaciones en la quimica fina. Essenze Derivati Agrumari, v.16, n.1, p.55-60, 1994.
TAVARES, E.S.; LOPES, D., BIZZO, H.R., LAGE, C.L.S., LEITÃO, S.G. Kinetin enhanced linalool production by in vitro plantlets of Lippia alba. Journal of Essential Oil Research, v.16, n.5, p.405-408, 2004.
TAVARES, E.S.; JULIAO, L.S.; LOPES, D.; BIZZO, H.R.; LAGE, C.L.S.; LEITAO, S.G. Análise do óleo essencial de folhas de três quimiotipos de Lippia alba (MILL.) N. E. BR (Verbenaceae) cultivados em condições semelhantes. Revista Brasileira de Farmacognosia, v.15, n.1, 2005 (no prelo).
TITON, M.; XAVIER, A.; OTONI, W.C. Dinámica do enraizamento de microestacas e miniestacas de clones de Eucalyptus grandis. Revista Árvore, v.26, n.6, p.665-573, 2002.
VALE, T.G.; MATOS, F.J.A.; LIMA, T.C.M.; VIANA, G.S.B. Behavioral effects of essential oils from Lippia alba (Mill.) N. E. Brown chemotypes. Journal of Ethnopharmacology, v.167, p.127-133, 1998.
VERMA, N.; TRIPATHI, A.K.; PREJEPATI, V.; BAHAL, J.K.; BANSAL, R.P.; KHANUJA, S.P. S.; KUMAR, S. Toxicity of essential oil from Lippia alba towards stored grain insects. Journal of Medicinal and Aromatic Plant Sciences, v.22-23, n.4A-1A, p.117-119, 2000-2001.
VIANA, G.S.B.; DO VALE, T.G.; RAO, V.S.N.; MATOS, F.J.A. Analgesic and antiinflamatory effects of two chemotypes of Lippia alba: A comparative study. Pharmaceutical Biology, v.36, n.5, p.347-351, 1998.
WENDING, I.; XAVIER, A. Gradiente de maturação e rejuvenescimento aplicado em espécies florestais. Floresta e Ambiente, v.8, n.1, p.187-194, 2001.
WURYATMO, E.; KLIEBER, A.; SCOTT, E.S. Inhibition of citrus postharvest pathogens by vapor of citral and related compounds in culture. Journal of Agricultural and Food Chemistry, v.51, n.9, p.2637-2640, 2003.
ZÉTOLA, M.; DE LIMA, T.C.M.; SONAGLIO, D.; GONZÁLES-ORTEGA, G.; LIMBERGER, R.P.; PETROVICK, P.R.; BASSANI, V.L. CNS activities of liquid and spray-dried extracts from Lippia alba - Verbenaceae (Brasilian false melissa). Journal of Ethnopharmacology, v.82, p.207-215, 2002.
ZOGHBI, M.G.B., ANDRADE, E.H.A., SANTOS, A.S., SILVA, M.H.; MAIA, J.G.S. Essential oils of Lippia alba (Mill) N. E. Br Growing wild in the Brazilian Amazon. Flavour and Fragrance Journal, v.13, p.47-48, 1998.

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E. S. Tavares
Instituto de Biologia CCS, UFRJ; Programa de Pósgraduação em Biotecnologia Vegetal, CCS, UFRJ Rua Brigadeiro Trompowsky S/N, Ilha do Fundão, 21941-590 Rio de Janeiro, RJ.
S. G. Leitão
Faculdade de Farmácia, CCS, UFRJ; 3Instituto de Biofísica, CCS, UFRJ; Programa de Pósgraduação em Biotecnologia Vegetal, CCS, UFRJ Rua Brigadeiro Trompowsky S/N, Ilha do Fundão, 21941-590 Rio de Janeiro, RJ.
F. Reinert
Instituto de Biologia CCS, UFRJ; Programa de Pósgraduação em Biotecnologia Vegetal, CCS, UFRJ Rua Brigadeiro Trompowsky S/N, Ilha do Fundão, 21941-590 Rio de Janeiro, RJ.
C. L. S. Lage
Instituto de Biofísica, CCS, UFRJ; Programa de Pósgraduação em Biotecnologia Vegetal, CCS, UFRJ Rua Brigadeiro Trompowsky S/N, Ilha do Fundão, 21941-590 Rio de Janeiro, RJ.

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Micropropagation of Three Chemotypes of Lippia Alba Mill. N. E. Br. Producers of Citral, Carvone and Linalool Respectively. Rev Fitos [Internet]. 2005 Jun. 30 [cited 2024 Nov. 21];1(01):67-73. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/17

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] ADAMS, R.M.; KOENIGSBERG, S.S.; LANGHANS, R.W. In vitro propagation of Cephallotus folicularis (Australian patcher plant). Horticultural Science, v.14, n.1, p.512-513, 1979.

[2] AGGARWAL, K.K; KHANUJA, S.P.S; AHMAD, A.; KUMAR, T.R.S; GUPTA, V.K.; KUMAR, S. Antimicrobial activity profiles of the two enantiomers of limonene and carvone isolated from the oils of Mentha spicata and Anethum sowa. Flavour and Fragrance Journal, v.17, n.1, p.59-63, 2002.

[3] ANDRADE, M.W.; LUZ, J.M.Q.; LACERDA, A.S.; MELO, P.R.A. Micropropagação da aroeira (Myracrodrun urundeuva Fr. All). Ciências Agrotécnicas, Lavras, v.24, n.1, p.174-180, 2000.

[4] CELIK, S.; OZYAKA, A. Effects of intraperitoneally administered lipoic acid, vitamin E and linalool on the level of total lipid and fatty acids in guinea pig brain with oxidative stress induced by H2O2. Journal of Biochemistry and Molecular Biology, v.35, n.6, p.547-552, 2002.

[5] CHIANG, L.C.; CHIANG, W.; CHANG, M.Y.; NG, L.T.; LIN, C.C. Antileukemic activity of selected natural products in Taiwan. American Journal of Chinese Medicine, v.31, n.1, p.37-46, 2003.

[6] CORRÊA, C.B.V. Contribuição ao estudo de Lippia alba (Mill.) N. E. Br. Ex Britt & Wilson - erva-cidreira. Revista Brasileira de Farmácia, v.73, n.3, p.57-64, 1992.

[7] DIP, E.C.; TAVARES, E.S.; JULIÃO, L.S.; MATHEUS, M.E.; LEITÃO, S.G.; LAGE, C.L.S. Sedative properties of ethanolic extracts from Lippia alba Mill. N. E. Brown: comparison of the activities of three different chemotipes. 1o Simpósio

[8] Brasileiro em Química Medicinal. Novas Estratégias em Planejamento racional de Fármacos. Caxambu, Minas Gerais, 2001.

[9] ELIZABETSKY, E.; SILVA-BRUM, L. F.; SOUZA, D.O. Anticonvulsivant properties of linalool in glutamate-related seizure models. Phytomedicine, v.6, n.2, p.107-113, 1999.

[10] ELLIS, M.D.; BAXENDALE, F.P. Toxicity of seven monoterpenoids to tracheal mites (Acari: Tarsonemidae) and their honey bee (Hymenoptera: Apidae) hosts when applied as fumigants. Journal of Economic Entomology, v.90, n.5, p.1087-1091, 1997.

[11] FAROOQI, A.H.A.; FATIMA, S.; SHARMA, S.; KUMAR, S. Plant growth control properties of essential oils and their constituents. Journal of Medicinal and Aromatic Plants, v.22, n.1B, p.603-604, 2000.

[12] FRIGHETTO, N.; OLIVEIRA, J.G.; SIANI, A.C.; CHAGAS, K.C. Lippia alba Mill N. E. Br. (Verbenaceae) as a source of linalool. Journal of Essential Oil Research, v.10, p.578-580, 1998.

[13] GRATAPAGLIA, D.; MACHADO, M.A. Micropropagação In: TORRES, A.C.; STYER, L.; Buso, J.A. Cultura de tecidos e transformação genética de plantas. Brasília: Embrapa-SPI / Embrapa - CNPH, v.1, n.1, p.183-260, 1998.

[14] GROUT, B.W.W. Photosynthesis of regenerated plantlets in vitro, and stresses of transplanting. Acta Horticulturae, v. 30, p.120-135, 1988.

[15] GUPTA, S.K., SINGH, P.; BAJPAI, P.; RAM, G.; DIGVIJAI, S.; GUPTA, M.M.; JAIN, D.C.; KHANUJA, S.P.S., KUMAR, S. Morphogenetic variation for artemisinin and volatile oil in Artemisia annua. Industrial Crops and Products, v.16, p.2217-224, 2002.

[16] GURGEL, V.T.; COUTO, F.E.; SANTOS, J.G.; VIANA, G.S.B. Central effects of citral, myrcene and limonene, constituents of essential oil chemotypes from Lippia alba (Mill.) N. E. Brown. Phytomedicine, v.9, n.8, p.709-714, 2002.

[17] HEMPEL, F.D.; WELCH, D.R.; FELDMAN, L.J. Floral induction and determination: where is flowering controlled? Trends in Plant Science, v.5, n.1, p.17-21, 2000.

[18] HUANG, L.C. Rejuvenation of trees and other perennials for restoration of plant regeneration competence. In: TORRES, A. C., CALDAS, L. S. Técnicas e aplicações da cultura de tecidos em plantas. Brasília: ABCTP/EMBRAPACNPH, p.252-264, 1990.

[19] JULIANI JR, H.R.; KOROCH, A.R.; JULIANI, H.R.; TRIPPI, V.S. Micropropagation of Lippia junelliana (Mold.) Tronc. Plant Cell Tissue Organic Culture, v.59, p.175-179, 1999.

[20] KOKALIS, B.N.; MARTINEZ, O.N.; RODRIGUEZ, K.R.; KLOEPPER, J.W. Development of multi-component transplant mixes for suppression of Meloidogyne ingognita on tomato (Lycopersicum esculentum). Journal of Nematology, v.34, n.4, p.362-369, 2002.

[21] LAGE, C.L.S. Meio de cultura para a prevenção e/ou redução da oxidação de cultura de tecido. Depositante Universidade Federal do Rio de Janeiro, Pat. I. BR. 0106515-7, 2001.

[22] LIU, C.H.; MISHRA, A.K.; HE, B.; TAN, R.X. Composition and antifungal activity of essential oils from Artemisia princeps and Cinnamomum camphora. International Pest Control, v.43, n.2, p.72-74, 2001.

[23] MATOS, F.J.A. As ervas cidreiras do Nordeste do Brasil. Estudo de três quimiotipos de Lippia alba (Mill.) N. E. Brown (Verbenaceae) Parte II - Farmacoquímica. Revista Brasileira de Farmácia, v.77, n.4, p.137-141, 1996.

[24] MATOS, F.J.A.; MACHADO, M.I.L.; CRAVEIRO, A.A.; ALENCAR, J.W. Essential oil composition of two chemotypes of Lippia alba grown in Northeast Brazil. Journal of Essential Oil Research v.8, p.695-698, 1996.

[25] MELO, B.; PINTO, J.E.B.P.; LUZ, J.M.Q.; PEIXOTO, J.R.; JULIATTI, F.C. Diferentes antioxidantes no controle da oxidação, germinação e desenvolvimento das plântulas na cultura in vitro de embriões da guarirobeira. Ciências

[26] Agrotécnicas, Lavras, v.25, n.6, p.1301-1306, 2001.

[27] MURASHIGUE, T. & SKOOG, F. A revised medium for rapid growth and bioassays of tobacco tissue cultures. Physiologia Plantarum, v.15, p.473-479, 1962.

[28] OKA, Y.; NACAR, S; PUTIEVSKY, E.; RAVID, U.; YANIV, Z.; SPIEGEL, Y. Nematicidal activity of essential oils and their components against the rootknot nematode. Phytopatology, v.90, n.7, p.710-715, 2000.

[29] PEANA, A.T.; D'AQUILA, P.S.; PANIN, F.; CHESSA, M.L.; MORETTI, M.D.L.; SERRA, G.; PIPPIA, P. (-)-Linalool produces antinociception in two experimental models of pain. European Journal of Pharmacology, v.460, n.1, p.37-41, 2003.

[30] PEANA, A.T.; D'AQUILA, P.S.; PANIN, F.; SERRA, G.; MORETTI, M.D.L. Antiinflammatory activity of linalool and linalyl acetate constituents of essential oil. Phytomedicine, v.9, n.8, p.721-726, 2002.

[31] RETAMAR, J.A. Variaciones fitoquimicas de la especie Lippia alba (salvia morada) y sus aplicaciones en la quimica fina. Essenze Derivati Agrumari, v.16, n.1, p.55-60, 1994.

[32] TAVARES, E.S.; LOPES, D., BIZZO, H.R., LAGE, C.L.S., LEITÃO, S.G. Kinetin enhanced linalool production by in vitro plantlets of Lippia alba. Journal of Essential Oil Research, v.16, n.5, p.405-408, 2004.

[33] TAVARES, E.S.; JULIAO, L.S.; LOPES, D.; BIZZO, H.R.; LAGE, C.L.S.; LEITAO, S.G. Análise do óleo essencial de folhas de três quimiotipos de Lippia alba (MILL.) N. E. BR (Verbenaceae) cultivados em condições semelhantes. Revista Brasileira de Farmacognosia, v.15, n.1, 2005 (no prelo).

[34] TITON, M.; XAVIER, A.; OTONI, W.C. Dinámica do enraizamento de microestacas e miniestacas de clones de Eucalyptus grandis. Revista Árvore, v.26, n.6, p.665-573, 2002.

[35] VALE, T.G.; MATOS, F.J.A.; LIMA, T.C.M.; VIANA, G.S.B. Behavioral effects of essential oils from Lippia alba (Mill.) N. E. Brown chemotypes. Journal of Ethnopharmacology, v.167, p.127-133, 1998.

[36] VERMA, N.; TRIPATHI, A.K.; PREJEPATI, V.; BAHAL, J.K.; BANSAL, R.P.; KHANUJA, S.P. S.; KUMAR, S. Toxicity of essential oil from Lippia alba towards stored grain insects. Journal of Medicinal and Aromatic Plant Sciences, v.22-23, n.4A-1A, p.117-119, 2000-2001.

[37] VIANA, G.S.B.; DO VALE, T.G.; RAO, V.S.N.; MATOS, F.J.A. Analgesic and antiinflamatory effects of two chemotypes of Lippia alba: A comparative study. Pharmaceutical Biology, v.36, n.5, p.347-351, 1998.

[38] WENDING, I.; XAVIER, A. Gradiente de maturação e rejuvenescimento aplicado em espécies florestais. Floresta e Ambiente, v.8, n.1, p.187-194, 2001.

[39] WURYATMO, E.; KLIEBER, A.; SCOTT, E.S. Inhibition of citrus postharvest pathogens by vapor of citral and related compounds in culture. Journal of Agricultural and Food Chemistry, v.51, n.9, p.2637-2640, 2003.

[40] ZÉTOLA, M.; DE LIMA, T.C.M.; SONAGLIO, D.; GONZÁLES-ORTEGA, G.; LIMBERGER, R.P.; PETROVICK, P.R.; BASSANI, V.L. CNS activities of liquid and spray-dried extracts from Lippia alba - Verbenaceae (Brasilian false melissa). Journal of Ethnopharmacology, v.82, p.207-215, 2002.

[41] ZOGHBI, M.G.B., ANDRADE, E.H.A., SANTOS, A.S., SILVA, M.H.; MAIA, J.G.S. Essential oils of Lippia alba (Mill) N. E. Br Growing wild in the Brazilian Amazon. Flavour and Fragrance Journal, v.13, p.47-48, 1998.