Antimicrobial activity of hydroalcoholic extracts of Eugenia uniflora L (Myrtaceae) and Schinus molle L (Anacardiaceae)

Teacher Luiz Francisley de Paiva
OrcID
Fabio Luiz Pereira Ferreira
OrcID
Izaura de Lima Miranda
OrcID
Túlio Custódio Reis
OrcID

    Teacher Luiz Francisley de Paiva

    University of Sapucaí Valley, Research Laboratory. Mayor Tuany Toledo Avenue, even-numbered side, Fátima, CEP 37554-210, Pouso Alegre, MG, Brazil.

    OrcID https://orcid.org/0000-0001-6497-7468

    Graduated in Biological Sciences from the University of Vale do Sapucaí (2012), and in Radiology Technology from the Universidade Nove de Julho (2019), Specialist in Clinical Analysis (2015) and Master in Sciences Applied to Health (2019) from the University of Vale do Sapucaí. He has experience in microbiology with an emphasis on clinical and food microbiology. He is currently a professor of the Bachelor's degree in Nutrition and Technology in Gastronomy and a technical researcher at the Basic Research Laboratory at the University of Vale do Sapucaí. He works with susceptibility to antimicrobials with an emphasis on essential oils, biofilms and pathogenic factors associated with Candida.

    Fabio Luiz Pereira Ferreira

    Universidad de Vale do Sapucaí, Laboratorio de Investigación. Avenida Prefeito Tuany Toledo, lado par, Fátima, CEP 37554-210, Pouso Alegre, MG, Brasil.

    OrcID https://orcid.org/0009-0005-7713-4757

    Izaura de Lima Miranda

    University of Sapucaí Valley, Research Laboratory. Mayor Tuany Toledo Avenue, even-numbered side, Fátima, CEP 37554-210, Pouso Alegre, MG, Brazil.

    OrcID https://orcid.org/0009-0009-8953-3578

    He has a high school education from the Dr. José Marques de Oliveira State School (1990). He is currently an Assistant Secretary at the Municipal Government of Pouso Alegre, MG. He has experience in the area of ​​Botany.

    Túlio Custódio Reis

    São Paulo State University, Araraquara School of Pharmaceutical Sciences. School of Pharmaceutical Sciences - Unesp Campos Ville, CEP 14800-903, Araraquara, SP, Brazil.

    OrcID https://orcid.org/0000-0003-1827-9102

    Túlio Custodio Reis - Bachelor of Biological Sciences from the Faculty of Philosophy, Sciences and Letters of the University of Vale do Sapucaí (UNIVAS, MG, Brazil). Master's student in Biosciences and Biotechnology Applied to Pharmacy (BBAF), concentration area Parasitology. Works at the Laboratory of Biochemistry and Molecular Biology of Trypanosomatids of the Department of Clinical Analysis of the Faculty of Pharmaceutical Sciences of the São Paulo State University "Júlio de Mesquita Filho" (UNESP, SP, Brazil). Currently his research focuses on: 1. Solid-phase synthesis of cyclic and biotinylated peptides using the Fmoc strategy. 2. Use of cyclic peptides that interact with the surface of Leishmania as a tool to evaluate the dynamic interaction between Leishmania and macrophages. 3. Determination of the anti-Leishmania activity of natural and synthetic compounds. He is a full student representative of the Research Ethics Committee (CEP) (2023-) and full student representative of the Council of the Postgraduate Program in Biosciences and Biotechnology Applied to Pharmacy (BBAF) (2024-), was a full student representative of the Permanent Research Committee (CPP) (2023-2024) and alternate representative of the Congregation (2023-2024), all at the Faculty of Pharmaceutical Sciences of Araraquara (FCFar UNESP).

Keywords

Medicinal plants
Phytotherapy
Fungi
Bacteria

Abstract

The indiscriminate use of antimicrobials available in the market today has been contributing to the increase in the quantity of resistant pathogens. In recent years, significant attention has been given to the use of antimicrobial compounds of natural origin. This growing demand for new bioactive compounds stems from the knowledge that some medicinal plants often exhibit antimicrobial activity. Thus, the aim of this study was to evaluate the antimicrobial effect of hydroalcoholic extracts from the leaves of E. uniflora (Surinam cherry) and S. molle (Brazilian pepper tree) against clinically relevant microorganisms. The leaves were collected from adult plants, identified at the Herbarium of the University of Vale do Sapucaí, where a voucher specimen was deposited. The extract was prepared using the maceration technique in 70% ethanol. Antimicrobial activity was assessed using the disc diffusion method according to the Kirby-Bauer methodology. The microorganisms used were reference strains of: Escherichia coli ATCC8739, Staphylococcus aureus ATCC6538, Pseudomonas aeruginosa ATCC9027, Salmonella typhimurium ATCC14028, Candida albicans ATCC90028, Candida glabrata MYA2950, Candida Krusei ATCC6258, Candida parapsilosis ATCC22019, and Candida utilis ATCC9950. The extracts showed antifungal and antibacterial activity against the studied microorganisms. These findings contribute to the potential future use of these plants as antimicrobial agents.

References

  1. Gonçalves MAP. Microbiota - implicações na imunidade e no metabolismo. Porto. Portugal. 53f. Dissertação de Mestrado [Programa de pós-graduação em Ciências Farmacêuticas] - Departamento de ciências farmacêuticas, Universidade Fernando Pessoa, UFP, 2014. [https://bdigital.ufp.pt/handle/10284/4516].
  2. Soysa NS, Samaranayake LP, Ellepola ANB. Cytotoxic drugs, radiotherapy and oral candidiasis. Oral Oncology. 2004; 40(10): 971-978. ISSN: 1368-8375. [https://doi.org/10.1016/j.oraloncology.2003.12.013].
  3. Pereira JA. Atividade antifúngica do geraniol sobre leveduras multirresistentes do gênero candida e perfil farmacológico e toxicológico em estudos in silico. João Pessoa, PB. 97f. Tese de doutorado [Programa de pós-graduação em Produtos Naturais e Sintéticos Bioativos] - Centro de Ciências da Saúde, Universidade Federal da Paraíba, UFPB, 2017. [https://repositorio.ufpb.br/jspui/handle/tede/9504?locale=pt_BR].
  4. Oliveira DV. Análise e caracterização de isolados ambientais da família Enterobacteriaceae quanto à presença de genes de resistência a Β-lactâmicos. Porto Alegre, RS. 58f. Tese de doutorado [Programa de pós-graduação em Microbiologia Agrícola e do Ambiente] - Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, UFRGS, 2016. [https://www.lume.ufrgs.br/handle/10183/157914].
  5. Giolo MP, Svidzinski TIE. Fisiopatogenia, epidemiologia e diagnóstico laboratorial da candidemia. J Bras Patol Medic Lab. 2010; 46(3): 225-234. ISSN: 1676-2444. [https://doi.org/10.1590/S1676-24442010000300009].
  6. Brito GNB. Presença de Candida, Staphylococcus, Enterobacteriaceae e Pseudomonadaceae na cavidade bucal de pacientes HIV positivos. São José dos Campos. SP. 159f. Dissertação [Programa de pós-graduação em Biopatologia Bucal] Faculdade de Odontologia de São José dos Campos, Universidade Estadual Paulista, UNESP, 2006. [https://repositorio.unesp.br/handle/11449/87944].
  7. World Health Organization (WHO). Antimicrobial resistance: draft global action plan on antimicrobial resistance. Geneva, Switzerland: 2015. 28 p. [https://www.who.int/publications/i/item/9789241509763].
  8. Akinnibosun FI, Akinnibosun HA, Ibeh I, Osaghae F. Antibacterial activity of Phyllanthus amarus Schum and Thonn. on five vegetative organisms. Pl Archive. 2008; 8(2):563-568. ISSN: 0972-5210.
  9. Lorenzi H. Árvores brasileiras: manual de identificação e cultivo de plantas arbóreas nativas do Brasil. 3ª ed., Nova Odessa: Instituto Plantarum de estudos da flora, v. 2, 384 p. 2009. ISBN:978-85-86714-50-4.
  10. Oliveira GL, Rodrigues CJ, Santos PO, Silva LRC, Santos AL, Belasco IC. Plantas medicinais utilizadas nas práticas integrativas e complementares de saúde no Espaço Crescer, Alcobaça, Bahia. REVISE. 2020; 5: 195-218. ISSN: 2179-6572. [https://doi.org/10.46635/revise.v5ifluxocontinuo.1892].
  11. Castellucci S, Lima MIS, Nordi N, Marques JGW. Plantas medicinais relatadas pela comunidade residente na estação ecológica de Jataí, município de Luís Antônio/SP: uma abordagem Etnobotânica. Rev Bras Pl Medic. 2000; 3(1): 51-60. ISSN: 1983-084X. [https://www1.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo_5_v3_n1.pdf].
  12. Coan CM, Matias T. A utilização das plantas medicinais pela comunidade indígena de Ventarra Alta-RS. SaBios-Rev Saúde Biol. 2014; 9(1):11-19. ISSN: 1980-0002. [https://revista2.grupointegrado.br/revista/index.php/sabios/article/view/958/571].
  13. Gaiola L, Cardoso CAL. Fitoquímica: potencialidades biológicas dos biomas brasileiros. In: Eugenia uniflora L. - uma espécie popular brasileira com potencial para desenvolvimento de produtos: uma revisão sistemática. 228 p. Editora Científica Digital. São Paulo, 2022. ISBN 978-65-5360-170-3. [http://dx.doi.org/10.37885/220207699].
  14. Silveira RM, Carvalho AFU, Bunguer MDO, Costa IRD. Diversidade da Composição Química dos Óleos Essenciais de Eugenia – Myrtaceae: uma revisão. Braz J Develop. 2021; 7(3): 33276-33303. ISSN: 2525-8761. [http://dx.doi.org/10.34117/bjdv7n3-855].
  15. Filho ACPM. Schinus molle e Schinus terebinthifolius: Revisão sistemática da classificação, e aspectos químicos, fitoquímicos, biológicos e farmacobotânicos. Braz J Nat Sci. 2020; 3(3): 490-513. ISSN: 2595-0584. [https://doi.org/10.31415/bjns.v3i3.109].
  16. Magne Machaca RW. Caracterizacion dendrologica y física de lasemilla de dos especies de arbolesforestales molle (Schinus molle) y fresno (Fraxinus excelsior). La Paz. Bolívia. 116f. Tese de Doutorado [Programa de pós-graduação em Engenharia Agronômica] - Universidad Mayor de San Andrés, Facultad de Agronomía, 2018. [https://repositorio.umsa.bo/handle/123456789/20116].
  17. Requejo Quispe EE. Actividad antibacteriana in vitro del aceite esencial de las hojas de Schinus molle l. (Molle) frente a cultivos de Staphylococcus aureus. Trujillo. Peru. 51f. Tese apresentada ao programa de pós-graduação em Química Farmacêutica, Facultad Ciencias de La Salud, Universidad Católica Los Ángeles de Chimbote, ULADECH, 2019. [http://repositorio.uladech.edu.pe/bitstream/handle/123456789/9007/ACEITE_ANTIBACTERIANO_REQUEJO_QUISPE_ENRIQUE_EMANUEL.pdf?sequence=1].
  18. Calderón Cóndor KR, Cristóbal Reyna ES. Actividad antibacteriana in vitro del extracto hidroalcohólico de las hojas de Schinus molle L. (Molle) sobre cepas de Staphylococcus aureus ATCC 6538 y Pseudomonas aeruginosa ATCC 9027. Lima. Perú. 103f. Tese [Programa de pós-graduação em Química Farmacêutica e Bioquímica] - Facultad de Ciencias Farmacéuticas y Bioquímica, Universidad Inca Garcilaso de La Veja, 2020. [http://repositorio.uigv.edu.pe/handle/20.500.11818/5111].
  19. Zambrano A. Actividad antimicótica" in vitro" del aceite esencial de fruto maduro de Schinus molle L. (Molle) frente a cepa de Candida albicans ATCC 10231. Lima. Peru. 79f. Tese [Programa de pós-graduação em Química Farmacêutica] - Facultad de Farmacia y Bioquímica, Universidad Norbert Wiener, 2019. [http://repositorio.uwiener.edu.pe/handle/123456789/3216].
  20. National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved Standard - Eighth Edition. NCCLS document M2-A8. National Committee for Clinical Laboratory Standards, Wayne, PA. 2003. ISBN:1-56238-485-6.
  21. Clinical and Laboratory Standards Institute. Method for antifungal disk diffusion susceptibility testing of yeast; approved guideline - 2nd ed. CLSI document M44-A2. Clinical and Laboratory Standards Institute, Wayne, PA. 2009. ISBN: 1-56238-703-0.
  22. Domingues SA, Paiva LF. Atividade antifúngica de Cymbopogon citratus (DC) Stapf frente à leveduras do gênero Candida sp. Rev Fitos. 2021; 15(1): 22-31. e-ISSN: 2446-477. [https://doi.org/10.32712/2446-4775.2021.958].
  23. Fiúza TS, Sabóia-Morais SMT, Paula JR, Tresvenzol LMF, Pimenta FC. Evaluation of antimicrobial activity of the crude ethanol extract of Eugenia uniflora L. leaves. Rev Ciênc Farmac Bás Apl. 2008; 29(3): 245-250. ISSN: 1808-4532 [https://rcfba.fcfar.unesp.br/index.php/ojs/article/view/468/466].
  24. Martins AS. Atividade antimicrobiana dos óleos essenciais de espécies do gênero Eugenia (Myrtaceae). Brasília. DF. 44f. Trabalho de conclusão de curso [Graduação em Farmácia] - Faculdade de Ciências da Saúde, Universidade de Brasília, UnB, 2015. [https://bdm.unb.br/bitstream/10483/12066/1/2015_AllandaSilvaMartins.pdf].
  25. Mendonça AT, Carvalho AR, Ferreira MC, Resende Júnior MC. A utilização dos extratos hidroalcoólico e alcoólico de Eugenia uniflora L. como agente antibacteriano. Rev Univ Vale Rio Verde. 2016; 14(1): 826-833. ISSN: 1517-0276. [https://doi.org/10.5892/ruvrd.v14i1.3019].
  26. Hasenclever L, Paranhos J, Costa CR, Cunha G, Vieira D. A indústria de fitoterápicos brasileira: desafios e oportunidades. Ciên Saúde Colet. 2017; 22(8): 2559-69. ISSN: 1678-4561. [https://doi.org/10.1590/1413-81232017228.29422016].
  27. Abílio VMF, Mesquita BS, Silva ED, Carvalho FVQ, Macêdo LLA, Castro RD. Actividad antifúngica de productos naturales indicados por vendedores de hierbas (raizeiros) para el tratamiento de la candidiasis oral. Rev Cub Estomatol. 2014; 51(3): 259-69. ISSN: 0034-7507. [http://scielo.sld.cu/pdf/est/v51n3/est03314.pdf].
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Author(s)

  • Teacher Luiz Francisley de Paiva
    University of Sapucaí Valley, Research Laboratory. Mayor Tuany Toledo Avenue, even-numbered side, Fátima, CEP 37554-210, Pouso Alegre, MG, Brazil.
    https://orcid.org/0000-0001-6497-7468
  • Fabio Luiz Pereira Ferreira
    Universidad de Vale do Sapucaí, Laboratorio de Investigación. Avenida Prefeito Tuany Toledo, lado par, Fátima, CEP 37554-210, Pouso Alegre, MG, Brasil.
    https://orcid.org/0009-0005-7713-4757
  • Izaura de Lima Miranda
    University of Sapucaí Valley, Research Laboratory. Mayor Tuany Toledo Avenue, even-numbered side, Fátima, CEP 37554-210, Pouso Alegre, MG, Brazil.
    https://orcid.org/0009-0009-8953-3578
  • Túlio Custódio Reis
    São Paulo State University, Araraquara School of Pharmaceutical Sciences. School of Pharmaceutical Sciences - Unesp Campos Ville, CEP 14800-903, Araraquara, SP, Brazil.
    https://orcid.org/0000-0003-1827-9102

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Antimicrobial activity of hydroalcoholic extracts of Eugenia uniflora L (Myrtaceae) and Schinus molle L (Anacardiaceae). Rev Fitos [Internet]. 2024 Dec. 18 [cited 2024 Dec. 22];18. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1718
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