Micropropagação e conservação de Lychnophora ericoides Mart.: uma espécie medicinal do cerrado brasileiro

A. M. S. Pereira; B. W. Bertoni; V. S. Fonseca; M. F. C. Amarante; N. P. Lopes; M. E. Paron; S. C. França

doi:10.32712/2446-4775.2005.27

In vitro propagation and germoplasm conservation of Lychnophora ericoides Mart: a medicinal species from the Brazilian cerrado

Vol. 1 No. 02 (2005)
69-73
11/10/2013
30/11/2005
https://doi.org/10.32712/2446-4775.2005.27

A. M. S. Pereira
B. W. Bertoni
V. S. Fonseca
M. F. C. Amarante
N. P. Lopes
M. E. Paron
S. C. França

Keywords

Cell culture

Arnica

Sesquiterpene lactones

Secondary metabolites

Abstract

Axillary buds excised from Lychnophora ericoides in vitro plantlets were inoculated onto MS medium supplemented with different concentrations of benzylaminopurine (BAP) and indolacetic acid (IAA). Developed plantlets were transferred to MS medium supplemented with indol-3-butyl-acetic acid (IBA) or naphtalenacetic acid (NAA) for rooting. BAP at a concentration of 1,11 M promoted plant growth (1,02 cm). Murashige and Skoog half-strenght medium (MS/2) medium supplemented with indol-3-butyl-acétic acid (IBA, 4,93 M) was the most suitable for rooting. High Performance Liquid Chromatography (HPLC) analysis of the in vitro and ex vitro plant extracts showed the presence of sesquiterpene lactones. For germplasm maintenance, cultures of L. ericoides were stored on MS/2 with 3% (w/v) sucrose plus 4% (w/v) sorbitol and maintained viable at a low growth rate with no subcultures during 10 months.

References

BASTOS, M.; CERQUEIRA, S.; SOUZA, J.T.; JÚNIOR, R.A.; PEIXO- TO, A.B.F. Ação analgésica do extrato bruto aquoso liofilizado do caule e folhas de Lychnophora ericoides Mart (arnica). Ciência e Cultura, v.39, n.5/6, p.551-553, 1987.
BORELLA, C.B.; LOPES, J.L.C.; VICHNEWSKI, W.; CUNHA, W.R.; HERZ, W. Sesquiterpene lactones triterpenes and flavones from Lychnophora ericoides and Lychnophora pseudovilosissima. Biochemical Systematics and Ecology, v.26, p.671-676, 1998.
COLLIN, H.A.; EDWARDS, S. Plant Cell Culture Oxford: BIOS Scientific Publishers, p.83-90, 1998.
DOS SANTOS, P.A.; AMARANTE, M.F.; PEREIRA, A.M.; BERTONI, B.; FRANCA, S.C.; PESSOA, C.; DE MORAES, M.O.; COSTA-LOTU- FO, L.V.; PEREIRA, M.R.; LOPES, N.P. Production of an antiproliferati- ve furanoheliangolide by Lychnophora ericoides cell culture. Chemical & Pharmaceutical Bulletin, v.52, n.12, p.1433-1435, 2004.
GRAEL, C.F.; VICHNEWSKI, W.; SOUZA, G.E.; LOPES, J.L.; ALBU- QUERQUE, S.; CUNHA, R. A study of the trypanocidal and analgesic properties from Lychnophora granmongolense (Duarte) Semir , Leitão Filho. Phytotherapy Research , v.14, n.3, p.203-206, 2000.
BRASIL. Lei n.º 7.804/89 e n.º 7.951/89, Portaria nº 37, IBAMA, Ministério do Meio Ambiente, de 3 de abril de 1992.
LEIFERT, C.; WAITES, W.M. Dealing with microbial contaminants in plant tissue and cell culture: hazard analysis and critical control point. In: LUMSDEN, P.J.; NICHOLAR, J.R.; DAVIES, W.J. (eds) Physiology, Growth and Development of plants in culture. Kluwer Academic Pub. Netherlands, p.363-378, 1994.
MIGUEL, O.G.; LIMA, E.O.; MORAIS, V.M.F.; GOMES, S.T.A.; DELLE-MONACHE, F.; CRUZ, A.B.; CRUZ, R.C.B.; CHECHINEL, F.V. Antimicrobial Activity of Constituents Isolated from Lychnophora salicifolia (Asteraceae). Phytotherapy Research. v.10, p.694, 1996.
MITTERMEIER, R.A.; MYERS, M.; GIL, P.R.; MITTERMEIER, C.G. Hotspots Earth’s biologically richest and most endangered terrestrial ecoregion. Cemex , Conservation International Pub., p.134-159, 2000.
MURASHIGE, T.; SKOOG, F. A revised medium for rapid grow and bioassays with tobacco tissue cultures. Physiologia Plantarum, v.15, p.473-497, 1962.
OLIVEIRA, A.B.; SAÃšDE, D.A.; PERRY, K.S.P.; DÂMARIS, S.D.; RASLAN, D.S.; BOAVENTURA, M.A.D.; CHIARI, E. Trypanocidal sesquiterpenes from Lychnophora Species. Phytotherapy Research, v.10, p.292-294, 1996.
PARON, M.E.; LOPES, N.P.; MACHADO, J.O. Germinação de aquênios de Lychnophora ericoides Mart (Asteraceae) In: IV Jornada Paulista de Plantas Medicinais 12-15 de outubro, Ribeirão Preto, Livro de Resumo PN 6 08, 1999.
PETIT, G.R.; HERALD, D.L.; CRAGG, G.M.; RIDEOUT, J.A.; BROWN, P. Antineoplastic agents, 178. Isolation and structure of lychnostatins 1 and 2 from the South. American Lychnophora antillana. Journal of natural products, v.53, n.2, p.382-390, 1990.
RUNGELER, P.; CASTRO, V.; MORA, G.; GOREN, N.; VICH- NEWSKI, W.; PAHL, H.L.; MERFORT, I.; SCHMIDT, T.J. Inhibition of transcription factor NF-kappaB by sesquiterpene lactones: a proposed molecular mechanism of action. Bioorganic & medicinal chemistry, v.7, n.11, p.2343-2352, 1999.
SAKAMOTO, H.T.; FLAUSINO, D.; CASTELLANO, E.E.; STARK, C.B.; GATES, P.J.; LOPES, N.P. Sesquiterpene lactones from Lych- nophora ericoides. Journal of natural products ,v.66, n.5, p.693-695, 2003.
SBB - SOCIEDADE BRASILEIRA DE BOTÂNICA. Centuria Plan- tarum Brasiliensium Exstintionis Miniata, Brasília, p.176, 1992.
SCHUMACHER, H.M. Biotechnology in the production and con- servation of medicial plant In: AKERELE, O.; HEYWOOD, H.; SYNGE, H. (eds). The consevation of medicinal plant. Cambridge University Press, p.179-198, 1991.

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Author(s)

A. M. S. Pereira
Unidade de Biotecnologia, Universidade de Ribeirão Preto, UNAERP Avenida Costábile Romano, 2201, 14096-380 Ribeirão Preto, SP, Brasil
B. W. Bertoni
Unidade de Biotecnologia, Universidade de Ribeirão Preto, UNAERP Avenida Costábile Romano, 2201, 14096-380 Ribeirão Preto, SP, Brasil
V. S. Fonseca
Unidade de Biotecnologia, Universidade de Ribeirão Preto, UNAERP Avenida Costábile Romano, 2201, 14096-380 Ribeirão Preto, SP, Brasil
M. F. C. Amarante
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas Universidade de São Paulo, USP, Ribeirão Preto, SP
N. P. Lopes
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas Universidade de São Paulo, USP, Ribeirão Preto, SP
M. E. Paron
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas Universidade de São Paulo, USP, Ribeirão Preto, SP
S. C. França
Unidade de Biotecnologia, Universidade de Ribeirão Preto, UNAERP Avenida Costábile Romano, 2201, 14096-380 Ribeirão Preto, SP, Brasil

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In vitro propagation and germoplasm conservation of Lychnophora ericoides Mart: a medicinal species from the Brazilian cerrado. Rev Fitos [Internet]. 2005 Nov. 30 [cited 2024 Nov. 13];1(02):69-73. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/27

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] BASTOS, M.; CERQUEIRA, S.; SOUZA, J.T.; JÚNIOR, R.A.; PEIXO- TO, A.B.F. Ação analgésica do extrato bruto aquoso liofilizado do caule e folhas de Lychnophora ericoides Mart (arnica). Ciência e Cultura, v.39, n.5/6, p.551-553, 1987.

[2] BORELLA, C.B.; LOPES, J.L.C.; VICHNEWSKI, W.; CUNHA, W.R.; HERZ, W. Sesquiterpene lactones triterpenes and flavones from Lychnophora ericoides and Lychnophora pseudovilosissima. Biochemical Systematics and Ecology, v.26, p.671-676, 1998.

[3] COLLIN, H.A.; EDWARDS, S. Plant Cell Culture Oxford: BIOS Scientific Publishers, p.83-90, 1998.

[4] DOS SANTOS, P.A.; AMARANTE, M.F.; PEREIRA, A.M.; BERTONI, B.; FRANCA, S.C.; PESSOA, C.; DE MORAES, M.O.; COSTA-LOTU- FO, L.V.; PEREIRA, M.R.; LOPES, N.P. Production of an antiproliferati- ve furanoheliangolide by Lychnophora ericoides cell culture. Chemical & Pharmaceutical Bulletin, v.52, n.12, p.1433-1435, 2004.

[5] GRAEL, C.F.; VICHNEWSKI, W.; SOUZA, G.E.; LOPES, J.L.; ALBU- QUERQUE, S.; CUNHA, R. A study of the trypanocidal and analgesic properties from Lychnophora granmongolense (Duarte) Semir , Leitão Filho. Phytotherapy Research , v.14, n.3, p.203-206, 2000.

[6] BRASIL. Lei n.º 7.804/89 e n.º 7.951/89, Portaria nº 37, IBAMA, Ministério do Meio Ambiente, de 3 de abril de 1992.

[7] LEIFERT, C.; WAITES, W.M. Dealing with microbial contaminants in plant tissue and cell culture: hazard analysis and critical control point. In: LUMSDEN, P.J.; NICHOLAR, J.R.; DAVIES, W.J. (eds) Physiology, Growth and Development of plants in culture. Kluwer Academic Pub. Netherlands, p.363-378, 1994.

[8] MIGUEL, O.G.; LIMA, E.O.; MORAIS, V.M.F.; GOMES, S.T.A.; DELLE-MONACHE, F.; CRUZ, A.B.; CRUZ, R.C.B.; CHECHINEL, F.V. Antimicrobial Activity of Constituents Isolated from Lychnophora salicifolia (Asteraceae). Phytotherapy Research. v.10, p.694, 1996.

[9] MITTERMEIER, R.A.; MYERS, M.; GIL, P.R.; MITTERMEIER, C.G. Hotspots Earth’s biologically richest and most endangered terrestrial ecoregion. Cemex , Conservation International Pub., p.134-159, 2000.

[10] MURASHIGE, T.; SKOOG, F. A revised medium for rapid grow and bioassays with tobacco tissue cultures. Physiologia Plantarum, v.15, p.473-497, 1962.

[11] OLIVEIRA, A.B.; SAÃšDE, D.A.; PERRY, K.S.P.; DÂMARIS, S.D.; RASLAN, D.S.; BOAVENTURA, M.A.D.; CHIARI, E. Trypanocidal sesquiterpenes from Lychnophora Species. Phytotherapy Research, v.10, p.292-294, 1996.

[12] PARON, M.E.; LOPES, N.P.; MACHADO, J.O. Germinação de aquênios de Lychnophora ericoides Mart (Asteraceae) In: IV Jornada Paulista de Plantas Medicinais 12-15 de outubro, Ribeirão Preto, Livro de Resumo PN 6 08, 1999.

[13] PETIT, G.R.; HERALD, D.L.; CRAGG, G.M.; RIDEOUT, J.A.; BROWN, P. Antineoplastic agents, 178. Isolation and structure of lychnostatins 1 and 2 from the South. American Lychnophora antillana. Journal of natural products, v.53, n.2, p.382-390, 1990.

[14] RUNGELER, P.; CASTRO, V.; MORA, G.; GOREN, N.; VICH- NEWSKI, W.; PAHL, H.L.; MERFORT, I.; SCHMIDT, T.J. Inhibition of transcription factor NF-kappaB by sesquiterpene lactones: a proposed molecular mechanism of action. Bioorganic & medicinal chemistry, v.7, n.11, p.2343-2352, 1999.

[15] SAKAMOTO, H.T.; FLAUSINO, D.; CASTELLANO, E.E.; STARK, C.B.; GATES, P.J.; LOPES, N.P. Sesquiterpene lactones from Lych- nophora ericoides. Journal of natural products ,v.66, n.5, p.693-695, 2003.

[16] SBB - SOCIEDADE BRASILEIRA DE BOTÂNICA. Centuria Plan- tarum Brasiliensium Exstintionis Miniata, Brasília, p.176, 1992.

[17] SCHUMACHER, H.M. Biotechnology in the production and con- servation of medicial plant In: AKERELE, O.; HEYWOOD, H.; SYNGE, H. (eds). The consevation of medicinal plant. Cambridge University Press, p.179-198, 1991.