Avaliação da Atividade Antiinflamatória dos Óleos Essenciais de Cinco Espécies de Myrtaceae

M. G. M. O. Henriques; E. C. Rosas; M. C. Souza; A. C. Siani; M. F. S Ramos

doi:10.32712/2446-4775.2006.54

Evaluation of the Anti-inflammatory Activity of Essential Oils from Five Myrtaceae Species

Vol. 2 No. 02 (2006)
58-66
16/10/2013
01/09/2006
https://doi.org/10.32712/2446-4775.2006.54

M. G. M. O. Henriques
E. C. Rosas
M. C. Souza
A. C. Siani
M. F. S Ramos

Keywords

Anti-inflamatory Activity

Abstract

The essential oils obtained from leaves of Eugenia brasiliensis, E. involucrata, E. jambolana, Psidium guajava and P. widgrenianum (Myrtaceae) were extracted by steam distillation and analyzed by GC/MS and correlation of Retention Indices. The volatile constituents were assayed for their activity as anti-inflammatory agents in the zymosan and lipopolysaccharide (LPS)- induced inflammatory models. Pre-treatment of mice with any of the oils at up to 100 mg/Kg as liposomes, p. o. had no inhibitory effect on leukocyte accumulation or protein leakage in zymosan-induced-pleurisy. E. jambolana was however effective in inhibiting total leukocyte (up to 56% at 200 mg/Kg) and eosinophil migration in LPS-induced pleurisy (up to 74% at 100 mg/Kg), but no dose-response correlation was observed. P. widgrenianum showed inhibitory effect only on eosinophil migration (up to 70% at 100 mg/Kg). Both EJ and PW were also assayed for ability to inhibit in vitro nitric oxide production. EJ inhibited NO production potently (up to 100%) and in a dosedependent manner, whereas PW showed only an effect moderate (51%), when tested below the cytotoxic dose (25 Î¼g/well). Chromatographic profiles of the essential oils showed the predominance of sesquiterpenes in oils of E. brasiliensis, E. involucrata and P. guajava, whereas monoterpenes were more important in P. widgrenianum and predominant in E. jambolana.

References

ADAMS, R.P. Identification of Essential Oil Components by Gas Chromatography / Mass Spectrometry. Allured Pub. Corp.: Illinois, USA, 1995.
BOZZA, P.T.; CASTRO-FARIA-NETO, H.C.; PENIDO, C.; LARANJEIRA, A.P.; HENRIQUES, M.G.M.O.; SILVA, P.M.; MARTINS, M.A.; RIBEIRO-DOS-SANTOS, R.; CORDEIRO, R.S.B. Requirement for lymphocytes and resident macrophages in LPS-induced pleural eosinophil accumulation. Journal of Leukocyte Biology, v.56, p.151-158 1994. .
COOPER, A.M.; ADAMS, L.B.; DALTON, D.K.; APPELBERG, R.; EHLERS, S. IFN-ã and NO in mycobacterial disease: new jobs for old hands. Trends in Microbiology, v.10, p.221-226, 2002.
CORRÊA, P. Dicionário das Plantas Úteis do Brasil e das Exóticas Cultivadas. Imprensa Nacional: Ministério da Agricultura, Rio de Janeiro, v.IV, p.429-430, 1984.
CRAVEIRO, A.A.; FERNANDES, G.F.; ANDRADE, C.H.S.; MATOS, F.J.A.; ALENCAR, J.W.; MACHADO, M.I.L. Óleos Essenciais de Plantas do Nordeste. Editora UFC, Fortaleza, 1981.
CRAVEIRO, A.A.; ANDRADE, C.H.S.; MATOS, F.J.A.; ALENCAR, J.W.; MACHADO, M.I.L. Essential oil of Eugenia jambolana. Journal of Natural Products, v.46, p.591-592, 1983.
DOBROVOLOSKAIA, M.A.; VOGEL, S.N. Toll receptors, CD14, and macrophage activation and deactivation by LPS. Microbes and Infection, v.4, p.903-914, 2002.
GUPTA, M.P. (ed.). 270 Plantas Medicinales Iberoamericanas. Ed. CYTED-SECAB: Santafé de Bogotá, p.413-420, 1995.
GIL, M.L.; JIMÉNEZ, J.; OCETE, M.A.; ZARZUELO, A.; CABO, M.M. Comparative study of different essential oils of Bupleurum gibraltaricum Lamarck. Pharmazie, v.44, p.284-287, 1989.
HAJHASHEMI, V.; GHANNADI, A.; SHARIF, B. Anti-inflammatory and analgesic properties of the leaf extracts and essential oil of Lavandula angustifolia Mill. Journal of Ethnopharmacology, v.89. p.67-71, 2003.
HENRIQUES, M.G.M.O.; WEG, V.B.; MARTINS, M.A.; SILVA, P.M.R.; FERNANDES, P.D.; CORDEIRO, R.S.B.; VARGAFTIG, B.B. Differential inhibition by two hetrazepne PAF antagonists of acute inflammation in the mouse. British Journal of Pharmacology, v.99, p.164-168, 1990.
HENRIQUES, A.T.; SOBRAL, M.E.; CAUDURO, A.D.; SCHAPOVAL, E.E.S.; BASSANI, V.L.; LAMATY, G.; MENUT, C.; BESSIÃˆRE, J.M. Aromatic plants from Brazil. II. The chemical composition of some Eugenia essential oils. Journal of Essential Oil Research, v.5, p.501-505, 1993.
JI, X.; PU, Q.; GARRAFO, H.M.; PANNELL, L.K. The essential oil of the leaves of Psidium guajava L. Journal of Essential Oil Research, v.3, p.187-189, 1991.
LIMBERGER, R.P.; APEL, M.A.; SOBRAL, M.; SCHAPOVAL, E.E.S.; HENRIQUES, A.T. Investigação da atividade antimicrobiana do óleo volátil de espécies da família Myrtaceae. Revista Brasileira de Farmácia, v.79, p.49-52, 1998.
MARTIN, S.; OCETE, M.A.; GALVEZ, J.; JIMÉNEZ, J.; ZAZUELO, A. Anti-inflammatory activity of the essential oil of Bupleurum fruticescens. Planta Medica, v.59, p.533-536, 1993.
OCETE, M.A.; RISCO, S.; ZARZUELO, A.; JIMENEZ, J. Pharmacological activity of the essential oil of Bupleurum gibraltaricum: anti-inflammatory activity and effects on isolated uteri. Journal of Ethnopharmacology, v.25, p.305-313, 1989.
OLAJIDE, O.A.; AWE, S.O.; MAKINDE, J.M. Pharmacological studies on the leaf of Psidium guajava. Fitoterapia, v.70, p.25-31, 1999.
PENIDO, C.; CASTRO-FARIA-NETO, H.; LARANJEIRA, A.; ROSAS, E.C.; RIBEIRO-DOS-SANTOS, R.; BOZZA, P.T.; HENRIQUES, M.G.M.O. The role of gamma-delta T lymphocytes in lipopolysaccharide-induced eosinophil accumulation into the mouse pleural cavity. Journal of Immunology, v.159, p.853-860, 1997.
RAO, B.G.V.N.; NIGAM, S.S. The in vitro antimicrobial efficiency of essential oils. Indian Journal of Medical Research, v.58, p.627-633, 1970.
SANTOS, F.A.; COSTA, A.M.L.; RAO, V.S.N.; SILVEIRA, E.R. Estudo da atividade antiinflamatória e analgésica do óleo essencial de Psidium guayanensis Pers. (araçá-azedo). Anais do XVI Simpósio de Plantas Medicinais do Brasil: F-012, 1996a.
SANTOS, F.A.; RAO, V.S.N.; SILVEIRA, E.R. Atividade antinociceptiva resistente ao naxolone do óleo essencial de Psidium pholianum Berg (araçá-doce). Anais do XVI Simpósio de Plantas Medicinais do Brasil: F-050, 1996b.
SANTOS, F.A.; RAO, V.S.N.; SILVEIRA, E.R. Investigations on the antinociceptive effect of Psidium guajava leaf oil and its major constituents. Phytoteraphy Research, v.12, p.24-27, 1998.
SCHAPOVAL, E.E.S.; SILVEIRA, S.M.; MIRANDA, M.L.; ALICE, C.B.; HENRIQUES, A.T. Evaluation of some pharmacological activities of Eugenia uniflora L. Journa of Ethnopharmacology, v.44, p.137-142, 1994.
SCHMEDA-HIRSCHMANN G.; THEODULOZ, C.; FRANCO, L.; FERRO, E.; DE ARIAS, A.R. Preliminary pharmacological studies on Eugenia uniflora leaves: xanthine oxidase inhibitory activity. Journal of Ethnopharmacology, v.21, p.183-186, 1987.
SIANI, A.C.; RAMOS, M.F.S.; MENEZES-DE-LIMA JR., O.; RIBEIRO-DOS-SANTOS, R.; FERNANDEZ-FERREIRA, E.; SOARES, R.O.A.; ROSAS, E.C.; SUSUNAGA, G.S.; GUIMARÃES, A.C.; ZOGHBI, M.G.B.; HENRIQUES, M.G.M.O. Evaluation of antiinflamatory-related activity of essential oils from the leaves and resin of species of Protium. Journal of Ethnopharmacology, v.66, p.57-69, 1999.
SIANI, A.C.; SAMPAIO, A.L.F.; SOUZA, M.C.; HENRIQUES, M.G.M.O.; RAMOS, M.F.S. Óleos Essenciais: Potencial antiinflamatório. Biotecnologia, Ciência & Desenvolvimento, v.16, p.38-43, 2000.
SOUZA, M.C.; SIANI, A.C.; RAMOS, M.F.S.; MENEZES DELIMA JR., O.; HENRIQUES, M.G.M.O. Evaluation of anti-inflammatory activity of essential oils from two asteraceae species. Pharmazie, v.58, n.8, p.582-586, 2003.
SLOWING, K.; CARRETERO, E.; VILLAR, A. Anti-inflammatory activity of leaf extracts of Eugenia jambos in rats. Journal of Ethnopharmacology, v.43, p.9-11, 1994.
SPECTOR, W.G. The mediation of altered capillary permeability in acute inflammation. The Journal of Pathology and Bacteriology, v.72, p.367-380, 1956.
THEODULOZ, C.; FRANCO, L.; FERRO, E.; SCHMEDA-HIRSCHMANN, G. Xanthine oxidase inhibitory activity of Paraguayan Myrtaceae. Journal of Ethnopharmacology, v.24, p.179-183, 1988.
VERA, R.R.; LAURENT, S.J.; FRAISSE, D.J. The volatile leaf oil of Eugenia brasiliensis Lam. from Réunion Island. Journal of Essential Oil Research v.6, p.155-159, 1994.
WALLEY, K.R.; MCDONALD, T.E.; HIGASHIMOTO, Y.; HAYASHI, S. American Journal of Respiratory and Critical Care Medicine v.160, p.698-704, 1999.

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Author(s)

M. G. M. O. Henriques
Laboratório de Química de Produtos Naturais e Farmacologia Aplicada, Far-Manguinhos, FIOCRUZ Rua Sizenando Nabuco 100, 21041-250 Rio de Janeiro, RJ, Brasil
E. C. Rosas
Laboratório de Química de Produtos Naturais e Farmacologia Aplicada, Far-Manguinhos, FIOCRUZ Rua Sizenando Nabuco 100, 21041-250 Rio de Janeiro, RJ, Brasil
M. C. Souza
Laboratório de Química de Produtos Naturais e Farmacologia Aplicada, Far-Manguinhos, FIOCRUZ Rua Sizenando Nabuco 100, 21041-250 Rio de Janeiro, RJ, Brasil
A. C. Siani
Laboratório de Química de Produtos Naturais e Farmacologia Aplicada, Far-Manguinhos, FIOCRUZ Rua Sizenando Nabuco 100, 21041-250 Rio de Janeiro, RJ, Brasil
M. F. S Ramos
Departamento de Medicamentos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, CCS, Bloco K Cidade Universitária, 21941-590 Rio de Janeiro, Brasil

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Evaluation of the Anti-inflammatory Activity of Essential Oils from Five Myrtaceae Species. Rev Fitos [Internet]. 2006 Sep. 1 [cited 2025 Apr. 14];2(02):58-66. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/54

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] ADAMS, R.P. Identification of Essential Oil Components by Gas Chromatography / Mass Spectrometry. Allured Pub. Corp.: Illinois, USA, 1995.

[2] BOZZA, P.T.; CASTRO-FARIA-NETO, H.C.; PENIDO, C.; LARANJEIRA, A.P.; HENRIQUES, M.G.M.O.; SILVA, P.M.; MARTINS, M.A.; RIBEIRO-DOS-SANTOS, R.; CORDEIRO, R.S.B. Requirement for lymphocytes and resident macrophages in LPS-induced pleural eosinophil accumulation. Journal of Leukocyte Biology, v.56, p.151-158 1994. .

[3] COOPER, A.M.; ADAMS, L.B.; DALTON, D.K.; APPELBERG, R.; EHLERS, S. IFN-ã and NO in mycobacterial disease: new jobs for old hands. Trends in Microbiology, v.10, p.221-226, 2002.

[4] CORRÊA, P. Dicionário das Plantas Úteis do Brasil e das Exóticas Cultivadas. Imprensa Nacional: Ministério da Agricultura, Rio de Janeiro, v.IV, p.429-430, 1984.

[5] CRAVEIRO, A.A.; FERNANDES, G.F.; ANDRADE, C.H.S.; MATOS, F.J.A.; ALENCAR, J.W.; MACHADO, M.I.L. Óleos Essenciais de Plantas do Nordeste. Editora UFC, Fortaleza, 1981.

[6] CRAVEIRO, A.A.; ANDRADE, C.H.S.; MATOS, F.J.A.; ALENCAR, J.W.; MACHADO, M.I.L. Essential oil of Eugenia jambolana. Journal of Natural Products, v.46, p.591-592, 1983.

[7] DOBROVOLOSKAIA, M.A.; VOGEL, S.N. Toll receptors, CD14, and macrophage activation and deactivation by LPS. Microbes and Infection, v.4, p.903-914, 2002.

[8] GUPTA, M.P. (ed.). 270 Plantas Medicinales Iberoamericanas. Ed. CYTED-SECAB: Santafé de Bogotá, p.413-420, 1995.

[9] GIL, M.L.; JIMÉNEZ, J.; OCETE, M.A.; ZARZUELO, A.; CABO, M.M. Comparative study of different essential oils of Bupleurum gibraltaricum Lamarck. Pharmazie, v.44, p.284-287, 1989.

[10] HAJHASHEMI, V.; GHANNADI, A.; SHARIF, B. Anti-inflammatory and analgesic properties of the leaf extracts and essential oil of Lavandula angustifolia Mill. Journal of Ethnopharmacology, v.89. p.67-71, 2003.

[11] HENRIQUES, M.G.M.O.; WEG, V.B.; MARTINS, M.A.; SILVA, P.M.R.; FERNANDES, P.D.; CORDEIRO, R.S.B.; VARGAFTIG, B.B. Differential inhibition by two hetrazepne PAF antagonists of acute inflammation in the mouse. British Journal of Pharmacology, v.99, p.164-168, 1990.

[12] HENRIQUES, A.T.; SOBRAL, M.E.; CAUDURO, A.D.; SCHAPOVAL, E.E.S.; BASSANI, V.L.; LAMATY, G.; MENUT, C.; BESSIÃˆRE, J.M. Aromatic plants from Brazil. II. The chemical composition of some Eugenia essential oils. Journal of Essential Oil Research, v.5, p.501-505, 1993.

[13] JI, X.; PU, Q.; GARRAFO, H.M.; PANNELL, L.K. The essential oil of the leaves of Psidium guajava L. Journal of Essential Oil Research, v.3, p.187-189, 1991.

[14] LIMBERGER, R.P.; APEL, M.A.; SOBRAL, M.; SCHAPOVAL, E.E.S.; HENRIQUES, A.T. Investigação da atividade antimicrobiana do óleo volátil de espécies da família Myrtaceae. Revista Brasileira de Farmácia, v.79, p.49-52, 1998.

[15] MARTIN, S.; OCETE, M.A.; GALVEZ, J.; JIMÉNEZ, J.; ZAZUELO, A. Anti-inflammatory activity of the essential oil of Bupleurum fruticescens. Planta Medica, v.59, p.533-536, 1993.

[16] OCETE, M.A.; RISCO, S.; ZARZUELO, A.; JIMENEZ, J. Pharmacological activity of the essential oil of Bupleurum gibraltaricum: anti-inflammatory activity and effects on isolated uteri. Journal of Ethnopharmacology, v.25, p.305-313, 1989.

[17] OLAJIDE, O.A.; AWE, S.O.; MAKINDE, J.M. Pharmacological studies on the leaf of Psidium guajava. Fitoterapia, v.70, p.25-31, 1999.

[18] PENIDO, C.; CASTRO-FARIA-NETO, H.; LARANJEIRA, A.; ROSAS, E.C.; RIBEIRO-DOS-SANTOS, R.; BOZZA, P.T.; HENRIQUES, M.G.M.O. The role of gamma-delta T lymphocytes in lipopolysaccharide-induced eosinophil accumulation into the mouse pleural cavity. Journal of Immunology, v.159, p.853-860, 1997.

[19] RAO, B.G.V.N.; NIGAM, S.S. The in vitro antimicrobial efficiency of essential oils. Indian Journal of Medical Research, v.58, p.627-633, 1970.

[20] SANTOS, F.A.; COSTA, A.M.L.; RAO, V.S.N.; SILVEIRA, E.R. Estudo da atividade antiinflamatória e analgésica do óleo essencial de Psidium guayanensis Pers. (araçá-azedo). Anais do XVI Simpósio de Plantas Medicinais do Brasil: F-012, 1996a.

[21] SANTOS, F.A.; RAO, V.S.N.; SILVEIRA, E.R. Atividade antinociceptiva resistente ao naxolone do óleo essencial de Psidium pholianum Berg (araçá-doce). Anais do XVI Simpósio de Plantas Medicinais do Brasil: F-050, 1996b.

[22] SANTOS, F.A.; RAO, V.S.N.; SILVEIRA, E.R. Investigations on the antinociceptive effect of Psidium guajava leaf oil and its major constituents. Phytoteraphy Research, v.12, p.24-27, 1998.

[23] SCHAPOVAL, E.E.S.; SILVEIRA, S.M.; MIRANDA, M.L.; ALICE, C.B.; HENRIQUES, A.T. Evaluation of some pharmacological activities of Eugenia uniflora L. Journa of Ethnopharmacology, v.44, p.137-142, 1994.

[24] SCHMEDA-HIRSCHMANN G.; THEODULOZ, C.; FRANCO, L.; FERRO, E.; DE ARIAS, A.R. Preliminary pharmacological studies on Eugenia uniflora leaves: xanthine oxidase inhibitory activity. Journal of Ethnopharmacology, v.21, p.183-186, 1987.

[25] SIANI, A.C.; RAMOS, M.F.S.; MENEZES-DE-LIMA JR., O.; RIBEIRO-DOS-SANTOS, R.; FERNANDEZ-FERREIRA, E.; SOARES, R.O.A.; ROSAS, E.C.; SUSUNAGA, G.S.; GUIMARÃES, A.C.; ZOGHBI, M.G.B.; HENRIQUES, M.G.M.O. Evaluation of antiinflamatory-related activity of essential oils from the leaves and resin of species of Protium. Journal of Ethnopharmacology, v.66, p.57-69, 1999.

[26] SIANI, A.C.; SAMPAIO, A.L.F.; SOUZA, M.C.; HENRIQUES, M.G.M.O.; RAMOS, M.F.S. Óleos Essenciais: Potencial antiinflamatório. Biotecnologia, Ciência & Desenvolvimento, v.16, p.38-43, 2000.

[27] SOUZA, M.C.; SIANI, A.C.; RAMOS, M.F.S.; MENEZES DELIMA JR., O.; HENRIQUES, M.G.M.O. Evaluation of anti-inflammatory activity of essential oils from two asteraceae species. Pharmazie, v.58, n.8, p.582-586, 2003.

[28] SLOWING, K.; CARRETERO, E.; VILLAR, A. Anti-inflammatory activity of leaf extracts of Eugenia jambos in rats. Journal of Ethnopharmacology, v.43, p.9-11, 1994.

[29] SPECTOR, W.G. The mediation of altered capillary permeability in acute inflammation. The Journal of Pathology and Bacteriology, v.72, p.367-380, 1956.

[30] THEODULOZ, C.; FRANCO, L.; FERRO, E.; SCHMEDA-HIRSCHMANN, G. Xanthine oxidase inhibitory activity of Paraguayan Myrtaceae. Journal of Ethnopharmacology, v.24, p.179-183, 1988.

[31] VERA, R.R.; LAURENT, S.J.; FRAISSE, D.J. The volatile leaf oil of Eugenia brasiliensis Lam. from Réunion Island. Journal of Essential Oil Research v.6, p.155-159, 1994.

[32] WALLEY, K.R.; MCDONALD, T.E.; HIGASHIMOTO, Y.; HAYASHI, S. American Journal of Respiratory and Critical Care Medicine v.160, p.698-704, 1999.