Emetine and cephaeline production and regulation by in vitro propagation of Psychotria ipecacuanha (Brot.) Stokes in semi-solid media and temporary immersion bioreactor

Simone da Silva
OrcID
Danielle Cardoso Alencar
OrcID
Paulo José Coelho Benevides
OrcID
Spartaco Astolfi-Filho
OrcID

    Simone da Silva

    Amazon Biotechnology Center, Plant Biotechnology Coordination, Avenida Governador Danilo de Matos Areosa, 690, Distrito Industrial, CEP 69075-351, Manaus, AM, Brazil.

    OrcID http://orcid.org/0000-0002-1818-283X

    Graduated in Bachelor of Biological Sciences (1999) and Licenciatura in Biological Sciences (2000), by the Federal University of the State of Rio de Janeiro - UNIRIO, with professional performance in the area of ​​Plant Biotechnology since 1997, with the development of activities of Scientific Initiation and Research in plant tissue culture, through the Phytopharmaceutical Quality Control Research Project at UFRJ (1998-2001). Master (2003) and Doctorate (2007) in Plant Biotechnology, from the Federal University of Rio de Janeiro - UFRJ, working on the In vitro Cultivation Projects of Medicinal Plants (2001-2007) and Induction of the production of toxic substances for vectors of tropical diseases in plants with insecticidal activity (2003-2006), both from UFRJ. In 2007, he started research and development activities at the Amazon Biotechnology Center, working on the implementation of research processes, development of protocols and large-scale production of medicinal, tropical ornamental, bromeliad and oil plants, using the culture technique of in 2013, assuming the coordination of the Plant Tissue Culture Laboratories. He served as Head of Department of Public Policies in Science, Technology and Innovation in the Executive Secretary of Planning, Science, Technology and Innovation of the State of Amazonas (2015-2016), aiming at the diversification of the economic matrix of the State. Currently, he coordinates the Plant Biotechnology area of ​​the Amazon Biotechnology Center, carrying out activities related to the development of plant tissue culture protocols of commercial interest, mainly in the Amazon Region.

    Danielle Cardoso Alencar

    Amazon Biotechnology Center, Coordination of Chemistry and Pharmacognosy, Avenida Governador Danilo de Matos Areosa, 690, Distrito Industrial, CEP 69075-351, Manaus, AM, Brazil.

    OrcID http://orcid.org/0000-0003-4013-3978

    Graduated in Chemistry from the Federal University of Amazonas (UFAM) (2007). He holds a Master's and PhD in Natural Products Chemistry from UFAM. He has experience in the isolation of active ingredients, analytical instrumentation (HPLC and MS) and structural determination (NMR 1D / 2D, UV, IR and MS), acting mainly on the following topics: phytochemical prospecting and investigation of bioactive substances of medicinal interest.

    Paulo José Coelho Benevides

    Amazon Biotechnology Center, Coordination of Chemistry and Pharmacognosy, Avenida Governador Danilo de Matos Areosa, 690, Distrito Industrial, CEP 69075-351, Manaus, AM, Brazil.

    OrcID http://orcid.org/0000-0002-6840-0890

    Bachelor in Chemistry from the Federal University of Rio de Janeiro (1990), Master in Organic Chemistry from the University of São Paulo (1996) and PhD in Organic Chemistry from the University of São Paulo (2001). Specialist and consultant to companies in natural product chemistry, innovation networks, sustainable development and socio-biodiversity product chains. Professor of postgraduate courses in Cosmetology, Perfumery and Trichology at Faculdades Oswaldo Cruz. Professional performance as a researcher / advisor, specialist and manager of research and product development teams in the industry and R, D & I centers with a primary focus on herbal medicines, medicinal chemistry, natural products chemistry, structuring of sustainable production chains and bioactive ingredients a from biodiversity. He served as executive technical advisor at the Amazon Biotechnology Center (CBA) from November 2015 to September 2017.

    Spartaco Astolfi-Filho

    Federal University of Amazonas, Institute of Biological Sciences, Department of Genetics, Av. Gen. Rodrigo Octávio, 6200, Coroado I, Rectory Building, 1st floor, North Sector, University Campus, CEP 69080-900, Manaus, AM, Brazil.

    OrcID http://orcid.org/0000-0001-7246-6350

    He graduated in Biological Sciences at UnB in 1975, Master in Molecular Biology at UnB 1978 and PhD in Sciences at UFRJ in 1987. He completed a postdoctoral degree in Genetic Engineering in 1988-1989 at the Institute of Science and Technology at the University of Manchester (UK). He was professor of Molecular Biology and Genetic Engineering at the University of Brasilia from 1978 to 1994 where he was head of the Department of Cellular Biology and coordinator of the Undergraduate Courses in Biological Sciences and Postgraduate Studies in Molecular Biology. In the aforementioned period, in collaboration with USP, for the first time it expressed in our country a eukaryotic gene (mouse alpha-amylase) in a host also eukaryotic (beer yeast), in collaboration with the UFRGS Biotechnology Center developed the technology of production of Taq-polymerase (the enzyme currently most used in molecular biology / genetic engineering) and together with BIOBRÁS - Biquímica do Brasil S / A developed the technology for the production of human insulin by genetic engineering / fermentation - this work culminated in the construction of an industrial unit for the production of human insulin in Montes Claros (MG), was also transferred to a Russian company. In 1995, he contributed to the development of biotechnology in the Amazon and transferred to the Federal University of Amazonas (UFAM) where he contributed to the implementation of a bold program for training human resources in biotechnology. Together with professionals from different institutions in the Amazon and other Brazilian regions, he created PROBEM / Amazônia (Brazilian Molecular Ecology Program for the Sustainable Use of Amazon Biodiversity), a Federal Government program with strong support from SUFRAMA, which aims at sustainable development of Amazon region through the implementation of Bioindustry Poles with environmentally friendly technologies. PROBEM / Amazônia's first concrete action was the construction of the Amazon Biotechnology Center (CBA). He supervised 77 dissertations and 45 theses, published 116 complete scientific articles in indexed journals and developed several biotechnological processes, 3 of which were grounds for granting international patents. Participated in the idealization, implementation and coordinated the Multi-Institutional Postgraduate Program in Biotechnology (PPG-BIOTEC) from 2002 to 2006, coordinated the Genomic Network of the Legal Amazon (REALGENE). He coordinated the Scientific Committee of the BIONORTE Network (2009-2011) and then was General Coordinator of the Graduate Program of the BIONORTE Network (PPG-BIONORTE) from 2012 to 2017. He is on the Scientific Council of the company Cristália - Produtos Químicos Farmacêuticos Ltda. He retired in 2017 as Professor of Genetic Engineering at the Department of Genetics at UFAM, where he continues to be Professor Emeritus.

Keywords

plant biotechnology
tissue culture
ipecac
emetine
cephaeline

Abstract

Psychotria ipecacuanha, is a plant species with known medicinal properties that is critically endangered due to overexploitation of natural populations. Although the difficulties in conventional propagation by seed and by vegetative propagation are generally understood, the present study enhances our knowledge by describing efficient plant regeneration and root induction protocols for P. ipecacuanha while comparing alkaloid content (emetine and cephaeline) in in vitro-derived tissues. Stem node explants were cultured on MS medium MS supplemented with indolbutiric acid (IBA) in semi-solid media and the RITA® temporary immersion bioreactor. The highest root formation (81%) was in MS + 1.5 mg L−1 IBA in the bioreactor. After 24 months of acclimatization, the plants cultivated in MS + 0.50 and 1.0 mg L-1 of IBA had the highest number of roots (3), with mean values of 10.47 and 9.40 cm, respectively. The cultures coming from 1.0 mg L−1 and 0.5 mg L−1 IBA in the bioreactor contained higher cephaeline content, with a relative area of 14.2 and 14.9%, respectively. For emetine, the 1.0 mg L−1 IBA cultures in the bioreactor, 0.5 mg L−1 IBA and MS0 cultures contained higher content than the other treatments, with a relative area of 10.2, 10.2 and 10.1%, respectively.

References

  1. Assis, MC, Giulietti, AM. Diferenciação morfológica e anatômica em populações de "ipecacuanha" - Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae). Braz J Botany. 1999; 22 (2): 205-216. ISSN 1806-9959. [CrossRef].
  2. Rossi AAB, Oliveira LO, Venturini BA, Silva RS. Genetic diversity and geographic differentiation of disjunct Atlantic and Amazonian populations of Psychotria ipecacuanha (Rubiaceae). Genetica - An Inter J Genetics Evol. 2009; 13: 57-67. ISSN 1573-6857. [CrossRef].
  3. Agra MF, Silva KN, Basílio IJLD, França PF, Barbosa-Filho JM. Survey of medicinal plants used in the region Northeast of Brazil. Rev Bras Farmacog. July/Sept. 2008; 18: 472-508. ISSN 1981-528X. [CrossRef].
  4. Burhans WC, Vassiley LT, Wu JM, Nallaseth FS, Depamphilis ML. Emetine allows identification of origins of mammalian DNA replication by imbalanced DNA synthesis, not through conservative nucleosome segregation. EMBO J. 1991; 10: 4351-4360. [CrossRef].
  5. Akinboye E, Bakare O. Biological activities of emetine. The Open Nat Prod J. 2011; 4: 8-15. ISSN 1874-8481. [Link] [CrossRef].
  6. Nomura T, Kutchan T. Three new o-methyltransferases are sufficient for all o-methylation reactions of ipecac alkaloid biosynthesis in root culture of Psychotria ipecacuanha. The J Biol Chem. 2010; 285 (10): 7722-7738. [CrossRef].
  7. Brandão M, Zanetti N, Oliveira A, Grael C, Santos A, MonteMór R. Brazilian medicinal plants described by 19th century European naturalists and in the Official Pharmacopoeia. J Ethnopharmacol. 2008; 120: 141–148. [CrossRef].
  8. Ideda K, Teshim D, Aoyama T, Satake M, Shimomura K. Clonal propagation of Cephaelis ipecacuanha. Plant Cell Reports. 1988; 7: 288-291. [CrossRef].
  9. Lameira OA. Cultivo da ipecacuanha [Psychotria ipecacuanha (Brot.) Stokes]. Belém: Embrapa Amazônia Oriental. Circular Técnica. 2002; 28: 1-4. [Link]
  10. Skorupa LA, Assis MC. Collection and conserving Ipecac (Psychotria ipecacuanha, Rubiaceae) germplasm in Brazil. Economic botany. 1998; 52 (2): 209-210. [CrossRef].
  11. Alves GR, Oliveira L, Alves MM, Silva BW. Variation in emetine and cephaeline contents in roots of wild Ipecac (Psychotria ipecacuanha). Biochem System Ecol. 2005; 33: 233-243. [CrossRef].
  12. Vieira LS. Manual da medicina popular. Belém: Agronomia Vozes. 1991; 247. [Link].
  13. Rocha TT, Lameira OA. Avaliação do período de floração e frutificação do BAG ipecacuanha. Em: 15° Seminário de Iniciação Científica da Embrapa. Belém, PA. Anais do 15° Seminário de Iniciação Científica da Embrapa: Embrapa Amazônia Oriental, 2011. [Link].
  14. Satoko I, Kaori T, Koichiro S. Gibberellic acid improved shoot multiplication in Cephaelis ipecacuanha. In vitro Cel Develop Biol Plant. 2007; 44 (3): 216-220. [CrossRef].
  15. Chatterjee K, Nandi P, Ghosh C. Cultivation and utilization of ipecac In West Bengal. In: Atal CK, Kapur BM. eds. Cultivation and utilization of medicinal plants. Jammu-Tawi, India: CSIR, Regional Research Laboratory. 1982; p.295-301. 877p. [Link].
  16. Rout GR, Samantaray S, Das P. In vitro somatic embryogenesis from callus cultures of Cephaelis ipecacuanha A. Richard. Sci Horticult. 2.000; 86: 71-79. [CrossRef].
  17. Jha S, Jha T. Micropropagation of Cephaelis ipecacuanha Rich. Plant Cell Reports. 1989; 8: 437-439. [CrossRef].
  18. Robinson P, John JBS, Balakrishnan V. Regeneration of Plants Through Somatic Embryogenesis in Emilia zeylanica C. B. Clarke a Potential Medicinal Herb. Bot Res Inter. 2009; 2 (1): 36-41. [Link].
  19. Khadke S, Kuvalekar A. Direct Somatic Embryogenesis and Plant Regeneration from Leaf and Stem Explants of Nothapodytes foetida: A Critically Endangered. Plant Species. Inter J Plant Anim Environ Sci. 2013; 3 (1): 257-264. ISSN 2231-4490. [Link].
  20. Aiqing J, Xueqing G, Yan Z, Hongyan Y, Guoliang W. Advances in Somatic Embryogenesis Research of Horticultural Plants. American J Plant Sci. 2011; 2: 727-732. [CrossRef].
  21. Rao SR, Ravishankar GA. Plant Cell Cultures: Chemical Factories of Secondary Metabolites. Biotechnol Adv. 2002; 20: 101-153. [CrossRef].
  22. Verpoorte R, Memelink J. Engineering secondary metabolite production in plants. Cur Oppin Biotechnol. 2002; 13: 181-187. [CrossRef].
  23. Lourenço MV. Biotecnologia de Plantas Medicinais: Produção de Biomoléculas. Biológico. 2003; 65 (1/2): 63-65. [Link].
  24. Silva S, Pinheiro EN, Assunção LM, Silva EL, Rodrigues DC, Ferreira FF, Astolfi-Filho S. In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid. Rev Fitos. 2018; 12 (3): 263-268. [CrossRef].
  25. Silva S, Astolfi-Filho S. Effect of indolebutyric acid on in vitro root production of Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae). Rev Fitos. 2018; 12 (3): 218-226. ISSN 2446-4775. [CrossRef].
  26. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plantarum. 1962; 15: 473-497. [Link].
  27. Oliveira ML, Xavier A, Filho RMP, Otoni WC, Teixeira JB. Efeitos do meio de cultura e da relação BAP/ANA na multiplicação in vitro de clones de Eucalyptus grandis x E. urophylla em bioreactor de imersão temporária. Rev Árvore. 2011; 35 (6): 1207-1217. [CrossRef].
  28. Berthouly M, Etienne H. Temporary immersion system: a new concept for use of liquid medium in mass propagation. In: International Symposium on Liquid Systems for in vitro Mass Propagation of Plants. Norway Proceedings. Norway: Cost 843 Working Group. 2002; 1: 37-38. [Link] [CrossRef].
  29. Watt MP. The status of temporary immersion system (TIS) technology for plant micropropagation. African J Biotechnol. 2012; 11 (76): 14025-14035. [CrossRef].
  30. Smet I, Beeckman T. Assymmetric cell division in land plants and algae: the driving force for differentiation. Nature Rev Mol Cell Biol. 2011; 12 (3): 177-188. [CrossRef].
  31. Li SW, Xue L, Xu S, Feng H, Na L. Mediators, genes and signaling in adventitious rooting. Botanycal Review. 2009; 75 (2): 230-247. ISSN 1874-9372. [CrossRef].
  32. Brondani GE, Baccarin FJB, Wit Ondas HW, Stape JL, Gonçalves AN, Almeida M. Low temperature, IBA concentrations and optimal time for adventitious rooting of Eucalyptus benthamii mini-cuttings. J Forestry Res. 2012: 23 (4): 583-592. [CrossRef].
  33. Brondani GE, Dutra LF, Wendling I, Grossi F, Hansel FA, Araujo MA. Micropropagation of an Eucalyptus hybrid (Eucalyptus benthamii x Eucalyptus dunnii). Acta Scient. 2011; 33 (4): 655-663. [CrossRef].
  34. Souza AV, Pereira MAS. Enraizamento de plantas cultivadas in vitro. Rev Bras Plantas Med. 2007; 9 (4): 103-117. [Link].
  35. Biddington NL, Thomas TH. A modified Amaranthus betacyanin bioassay for the rapid determination of cytokinins in plant extracts. Planta. 1973; 111: 183-186. [CrossRef].
  36. Mérillon JM, Liu D, Huguet F, Chénieux JC, Rideau M. Effect of calcium entry blockers and calmodulin inhibitors on cytokinin enhanced alkaloid accumulation in Catharanthus roseus cell cultures [indole alkaloids, calcium uptake]. Plant Physiol Biochem. 1991; 29: 289-296. [Link].
  37. Silva S, Lage CLS, Esquibel MA, Gil RASS, Sato A. In vitro propagation of Melissa officinalis L. and production of essential oil. Plant Cell Culture e Micropropag. 2006; 2(2): 53-60. [Link].
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Author(s)

  • Simone da Silva
    Amazon Biotechnology Center, Plant Biotechnology Coordination, Avenida Governador Danilo de Matos Areosa, 690, Distrito Industrial, CEP 69075-351, Manaus, AM, Brazil.
    http://orcid.org/0000-0002-1818-283X
  • Danielle Cardoso Alencar
    Amazon Biotechnology Center, Coordination of Chemistry and Pharmacognosy, Avenida Governador Danilo de Matos Areosa, 690, Distrito Industrial, CEP 69075-351, Manaus, AM, Brazil.
    http://orcid.org/0000-0003-4013-3978
  • Paulo José Coelho Benevides
    Amazon Biotechnology Center, Coordination of Chemistry and Pharmacognosy, Avenida Governador Danilo de Matos Areosa, 690, Distrito Industrial, CEP 69075-351, Manaus, AM, Brazil.
    http://orcid.org/0000-0002-6840-0890
  • Spartaco Astolfi-Filho
    Federal University of Amazonas, Institute of Biological Sciences, Department of Genetics, Av. Gen. Rodrigo Octávio, 6200, Coroado I, Rectory Building, 1st floor, North Sector, University Campus, CEP 69080-900, Manaus, AM, Brazil.
    http://orcid.org/0000-0001-7246-6350

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Emetine and cephaeline production and regulation by in vitro propagation of Psychotria ipecacuanha (Brot.) Stokes in semi-solid media and temporary immersion bioreactor. Rev Fitos [Internet]. 2020 Mar. 31 [cited 2026 Mar. 2];14(1):45-5. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/799
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