A cromatografia líquida a vácuo na análise qualitativa e quantitativa de flavonóides em Achyrocline satureioides

J. C. P. Mello; E. Saito; G. C. Lopes

doi:10.32712/2446-4775.2005.8

Vacuum Liquid Chromatography in the Qualitative and Quantitative Flavonoid Analyses in Achyrocline satureioides

Vol. 1 No. 01 (2005)
57-59
30/09/2013
30/06/2005
https://doi.org/10.32712/2446-4775.2005.8

J. C. P. Mello
E. Saito
G. C. Lopes

Keywords

Achyrocline satureoides

Asteraceae

3-O-methylquercetin

vaccum liquid chromatography

Abstract

The isolation of pharmacologically active compounds from vegetable sources is usually done by chromatographic methods, and the column chromatography is frequently employed. A variant of this methodology includes the vacuum liquid chromatography that could be used for qualitative or even quantitative analytic works, given that it presents high efficiency and low cost. The present study reports the isolation of 3-O-methylquercetin (1,21% yield) from semi-purified extracts of Achyrocline satureioides (Asteraceae) using the technique of vacuum liquid chromatography.

References

BAUER, L.; SIMÕES, C.M.O.; SIQUEIRA, N.C.S.; SCHENKEL, E.P.; SILVA, G.A.A.B.; ALICE, C.B. Flavonoids of the inflorescences of Achyrocline alata. Revista Latinoamericana de Química, v.18, n.3, p.91, 1987.
COLL, J.C.; BOWDEN, B.F. The application of vacuum liquid chromatography to the separation of terpene mixtures. Journal of Natural Products, v.49, n.5, p.934-936, 1986.
CUESTA-RUBIO, O.; VELEZ-CASTRO, H.; FRONTANA-URIBE, B.A.; CÁRDENAS, J. Nemorosone, the major constituent of floral resins of Clusia rosea. Phytochemistry, v.57, p.279-283, 2001.
DE SOUZA, K.C.B.; SCHAPOVAL, E.E.S.; BASSANI, V.L. LC determination of flavonoids: separation of quercetin, luteolin and 3-O-methylquercetin in Achyrocline satureioides preparations. Journal of Pharmaceutical and Biomedical Analysis, v.28, p.771-777, 2002.
FERRARO, G.E.; NORBEDO, C.; COUSSIO, J.D. Polyphenols from Achyrocline satureioides. Phytochemistry, v.20, n.8, p.2053-2054, 1981.
HOSTETTMANN, K.; QUEIROZ, E.F.; VIEIRA, P.C. Princípios ativos de plantas superiores. São Carlos: EdUFSSCar, 2003. p. 101-146.
MELLO, J.C.P.; PETROVICK, P.R. Quality control of Baccharis trimera (Less.) DC. (Astearaceae) hydroalcoholic extracts. Acta Farmaceutica Bonaerense, v.19, n.3, p.211-215, 2000.
PELLETIER, S.W.; CHOKSHI, H.P.; DESAI, H.K. Separation of diterpenoid alkaloid mixtures using vacuum liquid chromatography. Jornal of Natural Products, v.49, n.5, p.892-900, 1986.
ROITMAN, J.N.; JAMES, L.F. Chemistry of toxic range plants highly oxygenated flavonol methyl ethers from Gutierrezia microcephala. Phytochemistry, v.24, n.4, p.835-848, 1985.
SCHROEDER, D.R.; COLSON, K.L.; KLOHR, S.E.; LEE, M.S.; MATSON, J.A.; BRINEN, L.S.; CLARDY, J. Pyrrolosporin A, a new antitumor antibiotic from Micromonospora sp C39217-Rr109-7: II. Isolation, physico-chemical properties, spectroscopic study and X-ray analysis. Journal of Antibiotics, v.49, n.9, p.865-872, 1996.
SIMÕES, C.M.O.; BAUER, L. Investigação químico-farmacológica de Achyrocline satureioides (Lam.) DC., Compositae. In: SIMPÓSIO NACIONAL DE FARMACOLOGIA E QUÍMICA DE PRODUTOS NATURAIS, 2., 1983, João Pessoa, PB. Anais. João Pessoa:UFPB, 1983. p.415-419.
SZEPESI, G. HPLC in Pharmaceutical Analysis. Boca Raton: CRC Press, v.1, p.3-26, 1990.
TARGETT, N.M.; KILCOYNE, J.P.; GREEN, B. Vacuum liquid chromatography: an alternative to common chromatographic methods. Journal of Organic Chemistry, v.44, n.26, p.4962-4964, 1979.
VILLASENOR, I.M.; LEMON, P.; PALILEO, A.; BREMNER, J.B. Antigenotoxic spinasterol from Curcubita maxima flowers. Mutation Research, v.360, n.2, p.89-93, 1996.
VOIRIN, B.; JAY. M. Sur la présence de méthyl-3 quercétine chez Asplenium viride. Phytochemistry, v.13, p.275-276, 1974.

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Author(s)

J. C. P. Mello
Programa de Pós-graduação em Ciências Farmacêuticas Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá Av. Colombo 5790, 87020-900 Maringá, PR, Brasil
E. Saito
Departamento de Farmácia e Farmacologia – Universidade Estadual de Maringá
G. C. Lopes
Departamento de Farmácia e Farmacologia – Universidade Estadual de Maringá

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How to Cite

Vacuum Liquid Chromatography in the Qualitative and Quantitative Flavonoid Analyses in Achyrocline satureioides. Rev Fitos [Internet]. 2005 Jun. 30 [cited 2024 Nov. 24];1(01):57-9. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/8

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] BAUER, L.; SIMÕES, C.M.O.; SIQUEIRA, N.C.S.; SCHENKEL, E.P.; SILVA, G.A.A.B.; ALICE, C.B. Flavonoids of the inflorescences of Achyrocline alata. Revista Latinoamericana de Química, v.18, n.3, p.91, 1987.

[2] COLL, J.C.; BOWDEN, B.F. The application of vacuum liquid chromatography to the separation of terpene mixtures. Journal of Natural Products, v.49, n.5, p.934-936, 1986.

[3] CUESTA-RUBIO, O.; VELEZ-CASTRO, H.; FRONTANA-URIBE, B.A.; CÁRDENAS, J. Nemorosone, the major constituent of floral resins of Clusia rosea. Phytochemistry, v.57, p.279-283, 2001.

[4] DE SOUZA, K.C.B.; SCHAPOVAL, E.E.S.; BASSANI, V.L. LC determination of flavonoids: separation of quercetin, luteolin and 3-O-methylquercetin in Achyrocline satureioides preparations. Journal of Pharmaceutical and Biomedical Analysis, v.28, p.771-777, 2002.

[5] FERRARO, G.E.; NORBEDO, C.; COUSSIO, J.D. Polyphenols from Achyrocline satureioides. Phytochemistry, v.20, n.8, p.2053-2054, 1981.

[6] HOSTETTMANN, K.; QUEIROZ, E.F.; VIEIRA, P.C. Princípios ativos de plantas superiores. São Carlos: EdUFSSCar, 2003. p. 101-146.

[7] MELLO, J.C.P.; PETROVICK, P.R. Quality control of Baccharis trimera (Less.) DC. (Astearaceae) hydroalcoholic extracts. Acta Farmaceutica Bonaerense, v.19, n.3, p.211-215, 2000.

[8] PELLETIER, S.W.; CHOKSHI, H.P.; DESAI, H.K. Separation of diterpenoid alkaloid mixtures using vacuum liquid chromatography. Jornal of Natural Products, v.49, n.5, p.892-900, 1986.

[9] ROITMAN, J.N.; JAMES, L.F. Chemistry of toxic range plants highly oxygenated flavonol methyl ethers from Gutierrezia microcephala. Phytochemistry, v.24, n.4, p.835-848, 1985.

[10] SCHROEDER, D.R.; COLSON, K.L.; KLOHR, S.E.; LEE, M.S.; MATSON, J.A.; BRINEN, L.S.; CLARDY, J. Pyrrolosporin A, a new antitumor antibiotic from Micromonospora sp C39217-Rr109-7: II. Isolation, physico-chemical properties, spectroscopic study and X-ray analysis. Journal of Antibiotics, v.49, n.9, p.865-872, 1996.

[11] SIMÕES, C.M.O.; BAUER, L. Investigação químico-farmacológica de Achyrocline satureioides (Lam.) DC., Compositae. In: SIMPÓSIO NACIONAL DE FARMACOLOGIA E QUÍMICA DE PRODUTOS NATURAIS, 2., 1983, João Pessoa, PB. Anais. João Pessoa:UFPB, 1983. p.415-419.

[12] SZEPESI, G. HPLC in Pharmaceutical Analysis. Boca Raton: CRC Press, v.1, p.3-26, 1990.

[13] TARGETT, N.M.; KILCOYNE, J.P.; GREEN, B. Vacuum liquid chromatography: an alternative to common chromatographic methods. Journal of Organic Chemistry, v.44, n.26, p.4962-4964, 1979.

[14] VILLASENOR, I.M.; LEMON, P.; PALILEO, A.; BREMNER, J.B. Antigenotoxic spinasterol from Curcubita maxima flowers. Mutation Research, v.360, n.2, p.89-93, 1996.

[15] VOIRIN, B.; JAY. M. Sur la présence de méthyl-3 quercétine chez Asplenium viride. Phytochemistry, v.13, p.275-276, 1974.