Desenvolvimento e validação de um método por CCD/densitometria para quantificação de psoraleno e bergapteno em Brosimum gaudichaudii Trécul

Mariana Cristina de Moraes; Nathália Olívia Sousa Garcia; Bruno Lessa; Andréa Bezerra Da Nóbrega; Edemilson Cardoso da Conceição

doi:10.32712/2446-4775.2020.929

Development and validation of an HPTLC densitometry method for psoralen and bergapten quantification in Brosimum gaudichaudii Trécul

Vol. 14 No. 2 (2020)
165-176
14/12/2019
20/08/2020
https://doi.org/10.32712/2446-4775.2020.929

Mariana Cristina de Moraes
Nathália Olívia Sousa Garcia
Bruno Lessa
Andréa Bezerra Da Nóbrega
Edemilson Cardoso da Conceição

Keywords

Brosimum gaudichaudii

Abstract

Given the applicability of Brosimum gaudichaudii extracts in the treatment of vitiligo, the aim of the study was to develop a high performance thin layer chromatographic (HPTLC) analytical method for the simultaneous quantification of psoralen and bergapten, present in the lyophilized extract of B. gaudichaudii, in order to become an alternative method for quality control of extracts produced from this species. The lyophilized extract was produced from the soft extract of B. gaudichaudii roots, prepared with 55 % (v/v) hydroalcoholic solution. The methodology for the analysis of bergapten and psoralen compounds present in the lyophilized extract was developed and validated by thin layer chromatography (TLC)/densitometry. The best resolution, separation and identification of the markers occurred in RP 18 F254 (Merck) silica chromatoplate and on the mobile phase composed of acetonitrile: water (60:40). The retention factors (RF) for psoralen and bergapten were 0.41 and 0.35, respectively. The developed method of analysis was linear, precise and accurate. Thus, TLC/densitometry or HPTLC proved to be an alternative technique for the identification and separation of thesefuranocoumarins, being important for the development of new products containing B. gaudichaudii extracts for the treatment of vitiligo.

References

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Author(s)

Mariana Cristina de Moraes
UFG
Nathália Olívia Sousa Garcia
UFG
Bruno Lessa
Fundação Oswaldo Cruz
Andréa Bezerra Da Nóbrega
FIOCRUZ
Edemilson Cardoso da Conceição
UFG

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How to Cite

Development and validation of an HPTLC densitometry method for psoralen and bergapten quantification in Brosimum gaudichaudii Trécul. Rev Fitos [Internet]. 2020 Aug. 20 [cited 2024 Nov. 5];14(2):165-76. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/929

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] Del Río C, Millán E, García V, Appendino G, Demesa J, Muñoz E. The endocannabinoid system of the skin. A potential approach for the treatment of skin disorders. Biochem Pharmacol. 2018; 157: 122-133. ISSN 0006-2952. [CrossRef] [PubMed].

[2] Ma J, Li S, Zhu L, Guo S, Yi X, Cui T et al. Baicalein protects human vitiligo melanocytes from oxidative stress through activation of NF-E22-related factor 2 (Nrf2) signalling pathway. Free Radical Biology and Medicine. Free Rad Biol Med. 2018; 129: 492-503. ISSN 0891-5849. [CrossRef] [PubMed].

[3] Chatterjee M, Neema S. Dermoscopy of pigmentary disorders in brown skin. Dermatol clinics. 2018; 36(4): 473-485. ISSN 0733-8635. [CrossRef] [PubMed].

[4] Vallerand IA, Lewinson RT, Parsons LM, Hardin J, Haber RM, Lowerison MW et al. Vitiligo and major depressive disorder: A bidirectional population-based cohort study. J Am Acad Dermatol. 2019; 80(5): 1371-1379. ISSN 0190-9622. [CrossRef] [PubMed].

[5] Pozetti GL. Brosimum gaudichaudii Trécul (Moraceae): da planta ao medicamento. Rev Ciên Farm Bás Apl. 2005; 26 (3): 159. ISSN 2179-443X. [Link].

[6] Khovacs SR. Vitiligo. J Am Acad Dermatol. 1998; 38(5pt 1): 647-668. ISSN 0190-9622. [CrossRef] [PubMed].

[7] Steiner D, Villas RT, Bedin V, Steiner T, Moraes MB. Review Article: Vitiligo. Anais Bras Dermato. 2004; 79 (03): 335-351. ISSN 1806-4841.

[8] Ali J, Pramod K, Tahir MA. Current remedies for vitiligo. Autoimm Rev. 2010; 9: 516-520. ISSN 1568-9972. [CrossRef] [PubMed].

[9] Fatani MI, Alshrif SH, Alfif KA, Khan AS, Hussain WA, Banjar AA. The clinical patterns of vitiligo â€œhospital-based studyâ€ in Makah region, Saudi Arabia. J Dermato Dermatol Surgery. 2014; 18(1-2): 17-21. ISSN 2210-836X. [CrossRef].

[10] Ribeiro CVC, Kaplan MAC. Tendências evolutivas de famílias produtoras de cumarinas em Angiospermae. Quím Nova. 2002; 25(4): 533-538. ISSN 0100-4042.11. [CrossRef].

[11] Morais MCM, Almeida PHG, Ferreira NLO, Arruda RL, Borges LL, Freitas O, Conceição EC. Validation of a photostability indicating method for quantification of furanocoumarins from Brosimum gaudichaudii soft extract. Rev Bras Farmacog. 2018; 28(1): 118-123. ISSN 0102-695X. [CrossRef].

[12] Priyamvada S, Srinivas B, Pratima M. Qualitative high performance thin layer chromatography (HPTLC) analysis of cannabinoids in urine samples of Cannabis abusers. Indian J Med Res. 2010; 132: 201-208. ISSN 0971-5916. [PubMed].

[13] Domínguez A, Jarne C et al. A Hyphenated Technique based on High-Performance Thin Layer Chromatography for Determining Neutral Sphingolipids: A Proof of Concept. Chromat. 2015, 2: 167-187. ISSN 2227-9075. [CrossRef].

[14] Kerr E, West CY, Kradtap S. Quantitative TLC-Image Analysis of Urinary Creatinine Using Iodine Staining and RGB Values. J Chromat Sci. 2016; 54 (4): 639-646. ISSN 1945-239X. [CrossRef] [PubMed].

[15] Martins FS, Pascoa H, Paula JR, Conceição EC. Technical aspects on production of fluid extract from Brosimum gaudichaudii Trécul roots. Pharmacogn Mag. 2015; 11 (41): 226-231. ISSN 0973-1296. ISSN 0976-4062. [CrossRef] [PubMed].

[16] Agência Nacional de Vigilância Sanitária. ANVISA. Instrução Normativa nº 4, de 18 de junho de 2014. Guia de orientação para registro de Medicamento Fitoterápico e registro e notificação de Produto Tradicional Fitoterápico. Brasília: 2014. [Link].

[17] Agência Nacional de Vigilância Sanitária. Resolução RE 899, de 29 de maio de 2003. Guia para validação de métodos analíticos e bioanalíticos. D. O. U, Poder executivo, Brasília, DF, 02 de julho de 2003. [Link].

[18] Morais MC, Desenvolvimento e caracterização de dispersões sólidas contendo o extrato liofilizado de Brosimum gaudichaudii Trécul. (Moraceae) padronizado em psoraleno e bergapteno. 112p. 2019. Tese Doutorado [em Ciências Farmacêuticas] Faculdade de Farmácia. Universidade Federal de Goiás. 2019. [Link].

[19] Agência Nacional da vigilância Sanitária. Resolução da Diretoria Colegiada – RDC nº 166, de 24 de julho de 2017. Brasil, 2017. [Link].

[20] Alam PSNA, Al-rehaily AJ, Alajmi MF, Basudan OA, Khan TH. Stability indicating densitometric high-performance thin-layer chromatographic method for the quantitative analysis of biomarker naringin in the leaves and stems of Rumex vesicarius L. J Planar Chromatogr. 2014; 27 (3): 204-209. [CrossRef].

[21] Galand N, Pothier J, Viel C. Plant Drug Analysis by Planar Chromatography. J Chromatogr Sci. 2002, 40: 1-14. ISSN 1945-239X. [CrossRef] [PubMed].

[22] Dugo PM, Mondello L, Cogliandro E, Cavazza A, Dugoet G. On the genuineness of citrus essential oils. Part LIII. Determination of the composition of the oxygen heterocyclic fraction of lemon essential oils (Citrus Limon (L.) Burm. F.) by normal-phase high performance liquid chromatography. Flav Fragr J. 2000; 13 (5): 329-334. [CrossRef].

[23] Bolina RC, Garcia EE, Duarte MGR. Estudo comparativo da composição química das espécies vegetais Mikania glomerata Sprengel e Mikania laevigata Schultz Bip. ex Baker. Rev Bras Farmacogn. 2009; 19 (1B). ISSN 1981-528X. [CrossRef].