Avaliação da capacidade antioxidante, quantificação de fenólicos e perfil UV do extrato acetato de etila de Protium spruceanum Benth. Engl

Pedro Henrique Fonseca Veloso; Guilherme Araújo  Lacerda

doi:10.32712/2446-4775.2025.1825

Avaliação da capacidade antioxidante, quantificação de fenólicos e perfil UV do extrato acetato de etila de Protium spruceanum Benth. Engl

v. 19 (2025)
e1825
03/02/2025
09/12/2025
https://doi.org/10.32712/2446-4775.2025.1825

Pedro Henrique Fonseca Veloso
Guilherme Araújo Lacerda

Palavras-chave

Protium spruceanum

Fenólicos totais

Flavonoides

Capacidade antioxidante

Análise espectral

Resumo

A família botânica Burseraceae inclui o gênero Protium, que apresenta alta diversidade na América do Sul, com destaque para Protium spruceanum, conhecida por suas propriedades medicinais. Este estudo investigou a composição fitoquímica e a capacidade antioxidante do extrato acetato de etila dessa espécie. Foram quantificados fenólicos totais (302,35 mg EAG/g) e flavonoides (88,58 mg EqR/g e 59,74 mg EqQ/g), indicando uma rica presença de compostos bioativos. A capacidade antioxidante do extrato foi avaliada pelo ensaio DPPH, resultando em um valor de EC50 de 23,10 µg/mL. Embora inferior a outros extratos, esse resultado sugere uma atividade significativa. Além disso, a análise UV revelou picos de absorção em λ 279/217 nm, característicos de ácidos fenólicos e flavonóides, sugerindo uma complexidade estrutural dos metabolitos presentes. Esses achados reforçam o potencial de Protium spruceanum para aplicações terapêuticas, destacando sua relevância na medicina tradicional e moderna. A pesquisa contribui para a compreensão do solvente utilizado na extração, ressaltando a importância do acetato de etila na captura de uma diversidade de metabolitos. Futuros estudos devem focar nos mecanismos de ação dos compostos bioativos identificados, visando o desenvolvimento de novos agentes terapêuticos.

Referências

DeCarlo A, Dosoky NS, Satyal P, Sorensen A, Setzer WN. The Essential Oils of the Burseraceae [Internet]. Essent Oil Res. 2019; 61–145. Available from: [http://dx.doi.org/10.1007/978-3-030-16546-8_4].
Daly DCB, Fine PVA, Martínez-Habibe MC. Burseraceae: a model for studying the Amazon flora [Internet]. Rodriguésia. 2012; 63: 021–30. Available from: [http://dx.doi.org/10.1590/S2175-78602012000100002].
Silva DC, Sakuragui CM. Flora of Rio de Janeiro: Protium Burm. f. (Burseraceae) [Internet]. Rodriguésia. 2024; 75: e00012023. Available from: [http://dx.doi.org/10.1590/2175-7860202475018].
Flora do Brasil. Burseraceae in Flora e Funga do Brasil. Jardim Botânico do Rio de Janeiro. [acesso em: 09 set. 2024]. Disponível em: [https://floradobrasil.jbrj.gov.br/FB6588].
Brasil MCO, Lacerda GA, Arrudas SR, Veloso MDM. Essential Oil Content and Composition of Almescla Resin (Protium spruceanum Benth. Engl., Burseraceae [Internet]. J Agric Sci. 2018; 10: 442. Available from: [http://dx.doi.org/10.5539/jas.v10n11p442].
Rodrigues IV, Souza JNP, Silva ACG, Chibli LA, Cabral VAR, Viera Filho SA, et al. Antiedematogenic and antinociceptive effects of leaves extracts from Protium spruceanum Benth. (Engler) [Internet]. Pharmacogn J. 2013; 5: 6–12. Available from: [http://dx.doi.org/10.1016/j.phcgj.2012.08.001].
Amparo TR, Seibert JB, Mathias FAS, Vieira JFP, Soares RDOA, Freitas KM, et al. Anti-inflammatory activity of Protium spruceanum Benth. Engler is associated to immunomodulation and enzymes inhibition [Internet]. J Ethnopharmacol. 2019; 241: 112024. Available from: [http://dx.doi.org/10.1016/j.jep.2019.112024].
Amparo TR, Rodrigues IV, Seibert JB, Almeida TC, Cabral VAR, Vieira PM de A, et al. Antibacterial substances from leaves of Protium spruceanum (Burseraceae): in vitro and in silico evaluation [Internet]. Braz J Pharmac Sci. 2020; 56. Available from: [http://dx.doi.org/10.1590/s2175-97902020000118474].
Figueiredo JCG, Nunes YRF, de Oliveira Vasconcelos V, Arruda SR, Morais-Costa F, Santos GSC, et al. Effects of leaf extracts of Protium spruceanum against adult and larval Rhipicephalus microplus [Internet]. Experim Appl Acarol. 2019; 79: 447–58. Available from: [http://dx.doi.org/10.1007/s10493-019-00447-4]
Singleton VL, Orthofer R, Lamuela-Raventós RM. [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent [Internet]. Meth Enzymol. 1999; 152–78. Available from: [http://dx.doi.org/10.1016/s0076-6879(99)99017-1].
Pena Júnior DS, Almeida CA, Santos MCF, Fonseca PHV, Menezes EV, Melo Junior AF, et al. Antioxidant activities of some monofloral honey types produced across Minas Gerais (Brazil) [Internet]. Sarker U, editor. PLOS ONE. 2022; 17: e0262038. Available from: [http://dx.doi.org/10.1371/journal.pone.0262038].
Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity [Internet]. LWT - Food Sci Technol. 1995; 28: 25–30. Available from: [http://dx.doi.org/10.1016/s0023-6438(95)80008-5].
Rufino MDSM, et al. Metodologia científica: determinação da atividade antioxidante total em frutas pela captura do radical livre DPPH. 2007.
Santos LJ, Araujo ES, Lorenço MS, Rosário BB, Vieira SB, Sales A, et al. Bark of Protium tenuifolium Engl from the Amazonian biome as a source of phenolic extracts with antioxidant properties [Internet]. Res Square. 2023. Preprint. Available from: [http://dx.doi.org/10.21203/rs.3.rs-2674864/v1].
Islam MdR, Kabir MF, Alam R, Dhar R, Rana MN, Islam MdE, et al. Sedative, membrane stability, cytotoxic and antioxidant properties of methanol extract of leaves of Protium serratum Wall. [Internet]. Asian Pacific J Trop Disea. 2014; 4: S928–33. Available from: [http://dx.doi.org/10.1016/s2222-1808(14)60760-x].
Wang Z. Extract of Phenolics From Pomegranate Peels [Internet]. Open Food Sci J. 2011; 5: 17–25. Available from: [http://dx.doi.org/10.2174/1874256401105010017].
Amparo TR, Rodrigues IV, Seibert JB, Souza RHZ, Oliveira AR, Cabral VAR, et al. Antibacterial activity of extract and fractions from branches of Protium spruceanum and cytotoxicity on fibroblasts [Internet]. Nat Prod Res. 2017. 32: 1951–4. Available from: [http://dx.doi.org/10.1080/14786419.2017.1354182].
Amparo TR, Silva ACP, Seibert JB, Silva DS, Santos VMR, Vieira PMA, et al. In vitro and in silico investigation of the photoprotective and antioxidant potential of Protium spruceanum leaves and its main flavonoids [Internet]. J Photochem Photobiol A: Chem. 2022; 431: 114037. Available from: [http://dx.doi.org/10.1016/j.jphotochem.2022.114037].
Amparo T, Djeujo F, da Silva D, Seibert J, Rodrigues I, Santos O, et al. New Potential Use of Protium spruceanum in Hyperglycemia: α-Glucosidase Inhibition and Protection against Oxidative Stress [Internet]. J Braz Chem Soc. 2021. Available from: [http://dx.doi.org/10.21577/0103-5053.20210090]
Royo VA, Oliveira DA, Veloso PHF, Sacramento VM, Olimpio ELA, Souza LF, et al. Physicochemical Profile, Antioxidant and Antimicrobial Activities of Honeys Produced in Minas Gerais (Brazil) [Internet]. Antibiotics. 2022; 11: 1429. Available from: [http://dx.doi.org/10.3390/antibiotics11101429]
Alara OR, Abdurahman NH, Ukaegbu CI. Extraction of phenolic compounds: A review [Internet]. Curr Res Food Sci. 2021; 4: 200–14. Available from: [http://dx.doi.org/10.1016/j.crfs.2021.03.011]
Crozier A, Jaganath IB, Clifford MN. Dietary phenolics: chemistry, bioavailability and effects on health [Internet]. Nat Prod Rep. 2009; 26: 1001. Available from: [http://dx.doi.org/10.1039/b802662a].
Cosme P, Rodríguez AB, Espino J, Garrido M. Plant Phenolics: Bioavailability as a Key Determinant of Their Potential Health-Promoting Applications [Internet]. Antioxidants. 2020; 9: 1263. Available from: [http://dx.doi.org/10.3390/antiox9121263].
Olszowy-Tomczyk M, Typek R. Transformation of phenolic acids during radical neutralization [Internet]. J Food Sci Technol. 2023; 61: 790–7. Available from: [http://dx.doi.org/10.1007/s13197-023-05879-w].
Parri E, Santinami G, Domenici V. Front-Face Fluorescence of Honey of Different Botanic Origin: A Case Study from Tuscany (Italy) [Internet]. Applied Sci. 2020; 10: 1776. Available from: [http://dx.doi.org/10.3390/app10051776].
Ugaz OLS. Investigación fitoquímica: métodos en el estudio de productos naturales [Internet]. Departamento de Ciências. Pontificia Universidad Católica del Perú; 1994. Available from: [http://dx.doi.org/10.18800/9788483909522].
Donald LP, et al. Introduction to spectroscopy. 2015. eBook ISBN: 9781305177826.
Lourenço Neto M, Agra KL, Suassuna Filho J, Jorge FE. TDDFT calculations and photoacoustic spectroscopy experiments used to identify phenolic acid functional biomolecules in Brazilian tropical fruits in natura [Internet]. Spectrochimica Acta Part A: Molec Biomolec Spectr. 2018; 193: 249–57. Available from: [http://dx.doi.org/10.1016/j.saa.2017.12.036]
Pereira AIS, Pereira Guia AGS, Sobrinho OPL, Cantanhede EKP, Siqueira LFS. Atividade antimicrobiana no combate as larvas do mosquito Aedes aegypti: Homogeneização dos óleos essenciais do linalol e eugenol [Internet]. Educ Quím. Universidad Nacional Autonoma de Mexico. 2014; 25: 446–9. Available from: [http://dx.doi.org/10.1016/s0187-893x(14)70065-5].

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Autor(es)

Pedro Henrique Fonseca Veloso
Universidade Estadual de Montes Claros
https://orcid.org/0000-0003-2802-1244
Guilherme Araújo Lacerda
Universidade Estadual de Montes Claros
https://orcid.org/0000-0003-0878-6557

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Artigo visto 70 vez(es)

Como Citar

Avaliação da capacidade antioxidante, quantificação de fenólicos e perfil UV do extrato acetato de etila de Protium spruceanum Benth. Engl. Rev Fitos [Internet]. 9º de dezembro de 2025 [citado 13º de janeiro de 2026];19:e1825. Disponível em: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1825

Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.

[1] DeCarlo A, Dosoky NS, Satyal P, Sorensen A, Setzer WN. The Essential Oils of the Burseraceae [Internet]. Essent Oil Res. 2019; 61–145. Available from: [http://dx.doi.org/10.1007/978-3-030-16546-8_4].

[2] Daly DCB, Fine PVA, Martínez-Habibe MC. Burseraceae: a model for studying the Amazon flora [Internet]. Rodriguésia. 2012; 63: 021–30. Available from: [http://dx.doi.org/10.1590/S2175-78602012000100002].

[3] Silva DC, Sakuragui CM. Flora of Rio de Janeiro: Protium Burm. f. (Burseraceae) [Internet]. Rodriguésia. 2024; 75: e00012023. Available from: [http://dx.doi.org/10.1590/2175-7860202475018].

[4] Flora do Brasil. Burseraceae in Flora e Funga do Brasil. Jardim Botânico do Rio de Janeiro. [acesso em: 09 set. 2024]. Disponível em: [https://floradobrasil.jbrj.gov.br/FB6588].

[5] Brasil MCO, Lacerda GA, Arrudas SR, Veloso MDM. Essential Oil Content and Composition of Almescla Resin (Protium spruceanum Benth. Engl., Burseraceae [Internet]. J Agric Sci. 2018; 10: 442. Available from: [http://dx.doi.org/10.5539/jas.v10n11p442].

[6] Rodrigues IV, Souza JNP, Silva ACG, Chibli LA, Cabral VAR, Viera Filho SA, et al. Antiedematogenic and antinociceptive effects of leaves extracts from Protium spruceanum Benth. (Engler) [Internet]. Pharmacogn J. 2013; 5: 6–12. Available from: [http://dx.doi.org/10.1016/j.phcgj.2012.08.001].

[7] Amparo TR, Seibert JB, Mathias FAS, Vieira JFP, Soares RDOA, Freitas KM, et al. Anti-inflammatory activity of Protium spruceanum Benth. Engler is associated to immunomodulation and enzymes inhibition [Internet]. J Ethnopharmacol. 2019; 241: 112024. Available from: [http://dx.doi.org/10.1016/j.jep.2019.112024].

[8] Amparo TR, Rodrigues IV, Seibert JB, Almeida TC, Cabral VAR, Vieira PM de A, et al. Antibacterial substances from leaves of Protium spruceanum (Burseraceae): in vitro and in silico evaluation [Internet]. Braz J Pharmac Sci. 2020; 56. Available from: [http://dx.doi.org/10.1590/s2175-97902020000118474].

[9] Figueiredo JCG, Nunes YRF, de Oliveira Vasconcelos V, Arruda SR, Morais-Costa F, Santos GSC, et al. Effects of leaf extracts of Protium spruceanum against adult and larval Rhipicephalus microplus [Internet]. Experim Appl Acarol. 2019; 79: 447–58. Available from: [http://dx.doi.org/10.1007/s10493-019-00447-4]

[10] Singleton VL, Orthofer R, Lamuela-Raventós RM. [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent [Internet]. Meth Enzymol. 1999; 152–78. Available from: [http://dx.doi.org/10.1016/s0076-6879(99)99017-1].

[11] Pena Júnior DS, Almeida CA, Santos MCF, Fonseca PHV, Menezes EV, Melo Junior AF, et al. Antioxidant activities of some monofloral honey types produced across Minas Gerais (Brazil) [Internet]. Sarker U, editor. PLOS ONE. 2022; 17: e0262038. Available from: [http://dx.doi.org/10.1371/journal.pone.0262038].

[12] Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity [Internet]. LWT - Food Sci Technol. 1995; 28: 25–30. Available from: [http://dx.doi.org/10.1016/s0023-6438(95)80008-5].

[13] Rufino MDSM, et al. Metodologia científica: determinação da atividade antioxidante total em frutas pela captura do radical livre DPPH. 2007.

[14] Santos LJ, Araujo ES, Lorenço MS, Rosário BB, Vieira SB, Sales A, et al. Bark of Protium tenuifolium Engl from the Amazonian biome as a source of phenolic extracts with antioxidant properties [Internet]. Res Square. 2023. Preprint. Available from: [http://dx.doi.org/10.21203/rs.3.rs-2674864/v1].

[15] Islam MdR, Kabir MF, Alam R, Dhar R, Rana MN, Islam MdE, et al. Sedative, membrane stability, cytotoxic and antioxidant properties of methanol extract of leaves of Protium serratum Wall. [Internet]. Asian Pacific J Trop Disea. 2014; 4: S928–33. Available from: [http://dx.doi.org/10.1016/s2222-1808(14)60760-x].

[16] Wang Z. Extract of Phenolics From Pomegranate Peels [Internet]. Open Food Sci J. 2011; 5: 17–25. Available from: [http://dx.doi.org/10.2174/1874256401105010017].

[17] Amparo TR, Rodrigues IV, Seibert JB, Souza RHZ, Oliveira AR, Cabral VAR, et al. Antibacterial activity of extract and fractions from branches of Protium spruceanum and cytotoxicity on fibroblasts [Internet]. Nat Prod Res. 2017. 32: 1951–4. Available from: [http://dx.doi.org/10.1080/14786419.2017.1354182].

[18] Amparo TR, Silva ACP, Seibert JB, Silva DS, Santos VMR, Vieira PMA, et al. In vitro and in silico investigation of the photoprotective and antioxidant potential of Protium spruceanum leaves and its main flavonoids [Internet]. J Photochem Photobiol A: Chem. 2022; 431: 114037. Available from: [http://dx.doi.org/10.1016/j.jphotochem.2022.114037].

[19] Amparo T, Djeujo F, da Silva D, Seibert J, Rodrigues I, Santos O, et al. New Potential Use of Protium spruceanum in Hyperglycemia: α-Glucosidase Inhibition and Protection against Oxidative Stress [Internet]. J Braz Chem Soc. 2021. Available from: [http://dx.doi.org/10.21577/0103-5053.20210090]

[20] Royo VA, Oliveira DA, Veloso PHF, Sacramento VM, Olimpio ELA, Souza LF, et al. Physicochemical Profile, Antioxidant and Antimicrobial Activities of Honeys Produced in Minas Gerais (Brazil) [Internet]. Antibiotics. 2022; 11: 1429. Available from: [http://dx.doi.org/10.3390/antibiotics11101429]

[21] Alara OR, Abdurahman NH, Ukaegbu CI. Extraction of phenolic compounds: A review [Internet]. Curr Res Food Sci. 2021; 4: 200–14. Available from: [http://dx.doi.org/10.1016/j.crfs.2021.03.011]

[22] Crozier A, Jaganath IB, Clifford MN. Dietary phenolics: chemistry, bioavailability and effects on health [Internet]. Nat Prod Rep. 2009; 26: 1001. Available from: [http://dx.doi.org/10.1039/b802662a].

[23] Cosme P, Rodríguez AB, Espino J, Garrido M. Plant Phenolics: Bioavailability as a Key Determinant of Their Potential Health-Promoting Applications [Internet]. Antioxidants. 2020; 9: 1263. Available from: [http://dx.doi.org/10.3390/antiox9121263].

[24] Olszowy-Tomczyk M, Typek R. Transformation of phenolic acids during radical neutralization [Internet]. J Food Sci Technol. 2023; 61: 790–7. Available from: [http://dx.doi.org/10.1007/s13197-023-05879-w].

[25] Parri E, Santinami G, Domenici V. Front-Face Fluorescence of Honey of Different Botanic Origin: A Case Study from Tuscany (Italy) [Internet]. Applied Sci. 2020; 10: 1776. Available from: [http://dx.doi.org/10.3390/app10051776].

[26] Ugaz OLS. Investigación fitoquímica: métodos en el estudio de productos naturales [Internet]. Departamento de Ciências. Pontificia Universidad Católica del Perú; 1994. Available from: [http://dx.doi.org/10.18800/9788483909522].

[27] Donald LP, et al. Introduction to spectroscopy. 2015. eBook ISBN: 9781305177826.

[28] Lourenço Neto M, Agra KL, Suassuna Filho J, Jorge FE. TDDFT calculations and photoacoustic spectroscopy experiments used to identify phenolic acid functional biomolecules in Brazilian tropical fruits in natura [Internet]. Spectrochimica Acta Part A: Molec Biomolec Spectr. 2018; 193: 249–57. Available from: [http://dx.doi.org/10.1016/j.saa.2017.12.036]

[29] Pereira AIS, Pereira Guia AGS, Sobrinho OPL, Cantanhede EKP, Siqueira LFS. Atividade antimicrobiana no combate as larvas do mosquito Aedes aegypti: Homogeneização dos óleos essenciais do linalol e eugenol [Internet]. Educ Quím. Universidad Nacional Autonoma de Mexico. 2014; 25: 446–9. Available from: [http://dx.doi.org/10.1016/s0187-893x(14)70065-5].