Antimicrobial activity of Amazonian plant species against the causative agents of secondary infection in snakebites

Maria Carolina Scheffer de Souza
Valéria Mourão de Moura
Rosa Helena Veras Mourão
Maria Teresa Fachin-Espinar
Cecilia Veronica Nunez
Maria Cristina Dos-Santos

    Maria Carolina Scheffer de Souza

    Federal University of Amazonas (UFAM), Institute of Biological Sciences, Multi-institutional Graduate Program in Biotechnology - PPGBiotec, Avenida General Rodrigo Octavio Jordão Ramos, 1200, Bloco M, Coroado I, CEP 69067-005, Manaus, AM, Brazil.

    Graduated in Biological Sciences with an emphasis on Biotechnology from the University of Vale do Itajaí (2007), master's degree in Basic and Applied Immunology from the Federal University of Amazonas (2012) and doctorate in Biotechnology from the Federal University of Amazonas (2019). She has experience in bioassays with the cultivation of animal cells and microorganisms to assess the activity of natural products and poisons.

    Valéria Mourão de Moura

    Federal University of Western Pará - UFOPA, Bioprospecting and Experimental Biology Laboratory - LabBBEx, Avenida Mendonça Furtado, 2946, Fátima, CEP 68040-470, Santarém, PA, Brazil.

    He has a degree in Biological Sciences from Universidade Estadual Vale do Acaraú (2008) and a master's degree in Environmental Sciences from Universidade Federal do Oeste do Pará - UFOPA (2012). PhD in Biotechnology from the Federal University of Amazonas - UFAM (2016), with Post-doctorate from the State University of Amazonas -UEA, in partnership with the Tropical Medicine Foundation "Dr. Heitor Vieira Dourado" - FMT / HVD (2017). She is currently developing a post-doctorate in the Postgraduate Program in Natural Resources -PGRNA of the Federal University of Western Pará-UFOPA. His line of research is Bioprospection of Natural Products with an emphasis on plant extracts with blocking properties of poisonous snake toxins.

    Rosa Helena Veras Mourão

    Federal University of Western Pará - UFOPA, Bioprospecting and Experimental Biology Laboratory - LabBBEx, Avenida Mendonça Furtado, 2946, Fátima, CEP 68040-470, Santarém, PA, Brazil.

    Graduação em Ciências Biológicas pela Universidade Federal do Ceará (1993), mestrado em Bioquímica Vegetal pela Universidade Federal do Ceará (1996) e doutorado em Ciências Biológicas pela Universidade Federal de Pernambuco (2006) com bolsa sanduiche na Universidade Estadual de Campinas sob orientação do Prof Dr. Mário Saad. Professora associada nível 4 do Instituto de Saúde Coletiva da Universidade Federal do Oeste do Pará (UFOPA). Bolsista de Produtividade em Pesquisa (PQ2) do CNPq. Tem na área de Bioquímica, com destaque em Plantas Medicinais, atuando principalmente nos seguintes temas: diabetes, essenciais, atividade antiinflamatória, padronização de extratos, atividade antimicrobiana e veneno de B. atrox. É orientadora de mestrado nos Programas de Pós Graduação em Ciências da Saúde e Programa de Pós Graduação em Recursos Naturais (UFOPA) e doutorado no Programa de Pós Graduação em Biodiversidade e Biotecnologia (REDE BIONORTE).

    Maria Teresa Fachin-Espinar

    Amazonian National Research Institute (INPA), Bioprospection and Biotechnology Laboratory, Department for Technology and Innovation, Avenida André Araújo, 2936, Petrópolis, CEP 69067-375, Manaus, AM, Brazil.

    Graduated in Pharmacy and Biochemistry from the National University of the Peruvian Amazon (2008) with a diploma validated by the Federal University of Minas Gerais. Master in Biotechnology and Natural Resources of the Amazon from the State University of Amazonas (2015) PhD in Biotechnology from the Federal University of Amazonas. She has experience in bioprospecting, purification and characterization of biomolecules of natural products, conducting tests to evaluate chemical and biological activities. : antioxidant, antibacterial, toxicity and spectrometric and spectroscopic techniques: HPLC, NMR and mass spectrometry.

    Cecilia Veronica Nunez

    Amazonian National Research Institute (INPA), Bioprospection and Biotechnology Laboratory, Department for Technology and Innovation, Avenida André Araújo, 2936, Petrópolis, CEP 69067-375, Manaus, AM, Brazil.

    holds a degree in Chemistry from Universidade Mackenzie (1993), a master's degree in Organic Chemistry (Natural Products) from the University of São Paulo (1996), a PhD in Organic Chemistry (Natural Products) from the University of São Paulo (2000), post-doctorate in Chemistry Organic (Natural Products) from the University of São Paulo - São Carlos (2000-2001) and post-doctorate in Pharmacognosy from the Université de Lille-2, Droit et Santé, France (2009-2010). She is currently a Senior Technologist at the National Institute for Research in the Amazon and a permanent professor / advisor of the Postgraduate Programs in Biotechnology-UFAM / INPA, Biotechnology-UEA / INPA and Botany-INPA and collaborating professor / advisor of the Postgraduate Program in Chemistry-UFAM / INPA. She has been a Level 2 Productivity Scholar since 2014. She is the editor of the magazines Fitos and Vittalle and the special issue "Natural Products as less toxic Antitumor / Antiangiogenic Agents compared to Synthetic Conventional Chemotherapy" from Frontiers in Pharmacology (Qualis A1). She is a referee for Acta Amazonica, Química Nova, Revista Brasileira de Farmacognosia, Revista de Ciências Farmacêuticas Básicas e Aplicadas, International Research Journal of Biotechnology and Journal of Agricultural and Food Chemistry, among others. He has experience in the areas of Chemistry of Natural Products, Plant and Fungal Biotechnology, acting mainly on the following themes: bioprospecting of plants and endophytic microorganisms, plant biotechnology (obtaining calluses / culture of plant cells / cell suspensions), chromatographic fractionation and biomonitoring, antioxidant activity, antimicrobial activity, cytotoxic activity, chromatographic separation methodology, structural identification / elucidation of molecules by NMR, analysis of mixtures by NMR and photoionization of natural products. She is a member of the Interdisciplinary Committee for the Evaluation of Academic and Professional Graduate Books and Programs of the Coordination for the Improvement of Higher Education Personnel - CAPES. She has international collaboration with Prof. Dr. Olorunfemi Abraham Eseyin, from Nigeria, Dr. Jean Noel Nyemb, from Cameroon and Prof. Dr. Khaled Rashed, from Egypt to carry out the analysis and the structural identification / elucidation of the substances isolated by them.

    Maria Cristina Dos-Santos

    Immunochemistry Laboratory, Parasitology Department, Institute of Biological Sciences, Federal University of Amazonas, Manaus - UFAM, Manaus-AM, Brazil

Keywords

Bellucia dichotoma
Bothrops atrox
Snakebites
Infections
Antimicrobial
Wound healing

Abstract

Riverine communities in the Brazilian Amazon region use a variety of plants to treat snakebites. These plants can be effective against secondary infections, one of the main complications of snakebites. The aim of this study was to determine whether plants traditionally used to treat snakebites in the Brazilian Amazon may also have antimicrobial and antioxidant activities, and if so, which classes of chemicals may be responsible for these activities. Aqueous extracts of nine plants were tested in microdilution assays and the more active were prepared using solvents (hexane, methanol and water) and decoction, and nine assays were performed. Assays to determine the antioxidant activity of the most active species were carried out, as well as phytochemistry studies to determine the active components of this species. Bellucia dichotoma exhibited the greatest antimicrobial potential, particularly the hexane, methanol and decoction extracts. In comparative TLC, extracts of this species showed characteristics of terpenoids, compounds with double bonds and flavonoids. In 1H NMR, characteristic signals of sterols such as β-sitosterol, stigmasterols or triterpenes were observed, as well as signals indicating the presence of aromatic hydrogens, characteristic of aromatic substances, and sugars. The methanol extracts and decoction were considered active in the antioxidant assay.

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Author(s)

  • Maria Carolina Scheffer de Souza
    Federal University of Amazonas (UFAM), Institute of Biological Sciences, Multi-institutional Graduate Program in Biotechnology - PPGBiotec, Avenida General Rodrigo Octavio Jordão Ramos, 1200, Bloco M, Coroado I, CEP 69067-005, Manaus, AM, Brazil.
  • Valéria Mourão de Moura
    Federal University of Western Pará - UFOPA, Bioprospecting and Experimental Biology Laboratory - LabBBEx, Avenida Mendonça Furtado, 2946, Fátima, CEP 68040-470, Santarém, PA, Brazil.
  • Rosa Helena Veras Mourão
    Federal University of Western Pará - UFOPA, Bioprospecting and Experimental Biology Laboratory - LabBBEx, Avenida Mendonça Furtado, 2946, Fátima, CEP 68040-470, Santarém, PA, Brazil.
  • Maria Teresa Fachin-Espinar
    Amazonian National Research Institute (INPA), Bioprospection and Biotechnology Laboratory, Department for Technology and Innovation, Avenida André Araújo, 2936, Petrópolis, CEP 69067-375, Manaus, AM, Brazil.
  • Cecilia Veronica Nunez
    Amazonian National Research Institute (INPA), Bioprospection and Biotechnology Laboratory, Department for Technology and Innovation, Avenida André Araújo, 2936, Petrópolis, CEP 69067-375, Manaus, AM, Brazil.
  • Maria Cristina Dos-Santos
    Immunochemistry Laboratory, Parasitology Department, Institute of Biological Sciences, Federal University of Amazonas, Manaus - UFAM, Manaus-AM, Brazil

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Antimicrobial activity of Amazonian plant species against the causative agents of secondary infection in snakebites. Rev Fitos [Internet]. 2021 Sep. 30 [cited 2025 Jan. 18];15(3):280-97. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1084
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