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Influence of the extraction process on the antimicrobial activity of Croton antisyphiliticus Mart. (Euphorbiaceae)

    Teacher Amanda Malini Rocha Sales

    University of Brasilia (UNB), Institute of Biology, Darcy Ribeiro University Campus, Block E s/n 1st floor, Asa Norte, CEP 70910-900, DF, Brazil.

    OrcID https://orcid.org/0000-0003-1376-5339

    Pharmacist from the University of Brasília. Master in Pharmaceutical Sciences from UnB with a focus on the Development and Quality of Pharmaceutical Products and Services. She has experience in Pharmaceutical Technology, Hospital Pharmacy, Clinical Analysis and Scientific Dissemination. She has worked with Scientific Communication since 2016. She is a CNPq scholarship holder in the DTI-B modality working in the area of ​​Scientific Communication. She is a graduate student in Advertising and Propaganda at UnB. She is part of the Political-Normative Modernization of Brazilian Broadcasting project of the Communication Policy Laboratory (Lapcom/UnB). She was part of the Science and Culture projects with Revista Darcy (UnB) and BioUnB: Scientific dissemination on social networks.

    Mr. Matheus Eça de Oliveira Felipe

    Eastern Regional Hospital. Health Department of the Federal District. Northern Radio and TV Sector (SRTVN), 701 North, Via W5 North, lot D, CEP 70719-040, Brasília, Federal District, Brazil.

    OrcID https://orcid.org/0000-0002-5067-6389

    Farmacêutico Clínico Intensivista. Graduado pela Universidade de Brasília. Pós graduado pelo programa de residência multiprofissional em Terapia Intensiva da Escola Superior de Ciências da Saúde do Distrito Federal. Atualmente, trabalha na Secretaria de Saúde do Distrito Federal, atuando no Núcleo de Farmácia Clínica do Hospital da Região Leste.

    Dr. Eliana Fortes Gris

    University of Brasilia (UNB), Ceilândia Campus, Ceilândia South, Metropolitan Center, set A, lot 01, zip code 72220-275, Brasilia, DF, Brazil.

    OrcID https://orcid.org/0000-0003-0083-4075

    Graduated in Pharmacy and Food Technology from the Federal University of Santa Catarina (2001); Master in Food Science from the Federal University of Santa Catarina (2005); PhD in Food Science from the Federal University of Santa Catarina (2010). Currently Associate Professor at the University of Brasília (UnB), Ceilândia Campus (FCE), Pharmacy Course. Professor of the Postgraduate Program in Health Sciences and Technologies (PPGCTS), FCE, UnB. Works in the area of ​​Pharmacy, Food Science and Technology, Human Health, with emphasis on the following themes: food, bioactive compounds, antioxidant activity, phenolic compounds, chromatographic analysis, Cerrado fruits, wines, human health, aging, health technologies - including digital and gamification.

    Dr. Christopher William Fagg

    University of Brasilia (UNB), Institute of Biology, Darcy Ribeiro University Campus, Block E s/n 1st floor, Asa Norte, CEP 70910-900, DF, Brazil.

    OrcID https://orcid.org/0000-0001-6072-7832

    He holds a degree in Botany from the University of London (1976), a Master's degree in Applied Plant Sciences from the University of London (1979), and a PhD in Ecology from the University of Brasília (2001). He is currently an associate professor at the University of Brasília and an advisor to the Reference Center for Nature Conservation and Recovery of Degraded Areas (CRAD) at UnB. He is accredited in the Postgraduate Programs in Health Sciences and Technology and Botany (UnB). He works mainly on the following topics: Taxonomy of Leguminosae-Mimosoideae, floristics, soil-plant relationships, recovery of degraded areas, and identification and cultivation of medicinal plants.

    Teacher Daniel Oliveira Freire

    Planalto Central Apparecido dos Santos University Center (UNICEPLAC). SIGA Special Area for Industry Lot 2/3, Sce St. East Industrial, Gama, Zip Code 72445-020, Brasília, DF, Brazil.

    OrcID https://orcid.org/0000-0002-1577-7862

    Bachelor's degree in Biology from the Catholic University of Goiás. Specialist in Clinical Microbiology from the Faculty of Medicine of São José do Rio Preto, FAMP. Master's degree in Animal Biology from the University of Brasília. Currently a high school teacher at the State Department of Education of the Federal District. Served by the Health Department at the Clinical Pathology Center of the Gama Regional Hospital. Professor of Cytology, Clinical Microbiology and Bacteriology. Develops research in the area of ​​molecular identification of microorganisms and study of the antimicrobial resistance profile in bacteria of clinical interest.

    Dr. Izabel Cristina Rodrigues da Silva

    University of Brasilia (UNB), Ceilândia Campus, Ceilândia South, Metropolitan Center, set A, lot 01, zip code 72220-275, Brasilia, DF, Brazil.

    OrcID https://orcid.org/0000-0002-6836-3583

    She is an Associate Professor at the University of Brasília, in the Pharmacy course, Clinical Analysis Center. She is currently the coordinator of the Postgraduate Program in Health Science and Technology at UnB. She has a degree in Biomedicine from the São Paulo State University Júlio de Mesquita Filho (2000), a Master's degree in Sciences (Experimental Pathophysiology) from the University of São Paulo (2004) and a PhD in Molecular Pathology from the University of Brasília (2010). She is a Specialist in Public Health with an Emphasis on Health Surveillance (PUC-GO, 2012). In addition to her academic duties, she is currently seeking more knowledge. She is an undergraduate student of Chemistry at UNIP and of Data Science at Uninabuco. She has experience in the areas of Human and Medical Genetics and Biostatistics, in addition to Health Surveillance. She works on projects involving genetic polymorphisms, aspects of Health Surveillance and non-clinical studies. He is part of the startup MASQI.A INOVA SIMPLES (I.S.).

    Dr. Paula Melo Martins

    University of Brasilia (UNB), Ceilândia Campus, Ceilândia South, Metropolitan Center, set A, lot 01, zip code 72220-275, Brasilia, DF, Brazil.

    OrcID https://orcid.org/0000-0001-5824-9018

    Graduated in Pharmacy with a specialization in Industry from the Federal University of Minas Gerais (1994) and PhD in Plant Production from the Darcy Ribeiro State University of Northern Fluminense (2005). Scholarship holder of the CAPES/DAAD International Cooperation Program. Post-doctorate from the University of Pharmaceutical Sciences of Ribeirão Preto in the area of ​​pharmaceutical technology of plant products. Collaborating researcher at the Institute of Chemical Technology in Mumbai/India. Has experience in the area of ​​processing medicinal, aromatic and spice plants, with emphasis on quality control of raw materials and obtaining extracts. Develops several extension and research projects in the scope of integrative and complementary practices with emphasis on phytotherapy, Living Pharmacies and Ayurveda.

Keywords

Abstract

The present study aimed to evaluate the influence of the factors drug / solvent ratio (p/v) and time (days) in the maceration extraction process of Croton antisyphiliticus Mart. on antimicrobial activity and polyphenolic compound content. The extracts were obtained by maceration of the aerial parts, using a factorial design 32, considering 3 proportions 1:10, 1:20 and 1:30;  and 7, 10 and 13 days.  Extracts were characterized in total solids content, total polyphenols content and gallic acid.  Antimicrobial activity against Staphylococcus aureusand Escherichia coli was evaluated using the microdilution technique. Only the minimum inhibitory concentration (MIC) was shown to be influenced by the factors chosen, so that the higher the drug / solvent ratio and the longer the extraction time, the better the MIC values.  The F3 experiment (1:30 p/v and 13 days) obtained the best results in relation to the content of total polyphenols, gallic acid and MIC, with values ​​equal to 411.37 mg GAE / g, 75.97 mg GAE / g and 0.001  mg / mL, respectively.

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Author(s)

  • Teacher Amanda Malini Rocha Sales
    University of Brasilia (UNB), Institute of Biology, Darcy Ribeiro University Campus, Block E s/n 1st floor, Asa Norte, CEP 70910-900, DF, Brazil.
    https://orcid.org/0000-0003-1376-5339
  • Mr. Matheus Eça de Oliveira Felipe
    Eastern Regional Hospital. Health Department of the Federal District. Northern Radio and TV Sector (SRTVN), 701 North, Via W5 North, lot D, CEP 70719-040, Brasília, Federal District, Brazil.
    https://orcid.org/0000-0002-5067-6389
  • Dr. Eliana Fortes Gris
    University of Brasilia (UNB), Ceilândia Campus, Ceilândia South, Metropolitan Center, set A, lot 01, zip code 72220-275, Brasilia, DF, Brazil.
    https://orcid.org/0000-0003-0083-4075
  • Dr. Christopher William Fagg
    University of Brasilia (UNB), Institute of Biology, Darcy Ribeiro University Campus, Block E s/n 1st floor, Asa Norte, CEP 70910-900, DF, Brazil.
    https://orcid.org/0000-0001-6072-7832
  • Teacher Daniel Oliveira Freire
    Planalto Central Apparecido dos Santos University Center (UNICEPLAC). SIGA Special Area for Industry Lot 2/3, Sce St. East Industrial, Gama, Zip Code 72445-020, Brasília, DF, Brazil.
    https://orcid.org/0000-0002-1577-7862
  • Dr. Izabel Cristina Rodrigues da Silva
    University of Brasilia (UNB), Ceilândia Campus, Ceilândia South, Metropolitan Center, set A, lot 01, zip code 72220-275, Brasilia, DF, Brazil.
    https://orcid.org/0000-0002-6836-3583
  • Dr. Paula Melo Martins
    University of Brasilia (UNB), Ceilândia Campus, Ceilândia South, Metropolitan Center, set A, lot 01, zip code 72220-275, Brasilia, DF, Brazil.
    https://orcid.org/0000-0001-5824-9018

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Influence of the extraction process on the antimicrobial activity of Croton antisyphiliticus Mart. (Euphorbiaceae). Rev Fitos [Internet]. 2024 Aug. 30 [cited 2025 Apr. 5];18:e1188. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1188
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