Isolation of active antiphytopathogen compound from extracts of Anadenanthera colubrina var. cebil (Griseb.) Altschul

Cibele Maria Alves da Silva-Bessa
Carolina Barbosa Malafaia
OrcID
Paula Fernanda Figueiredo das Mercês
Daniel Rodrigo Cavalcante de Araújo
Ana Paula Sant'Anna da Silva
Vera Lúcia de Menezes Lima
Fabiane Rabelo da Costa Batista
Cláudia Sampaio de Andrade Lima
Elineide Barbosa de Souza
Maria Tereza dos Santos Correia
Maria Luiza Vilela Oliva

    Cibele Maria Alves da Silva-Bessa

    Federal University of Pernambuco (UFPE), Department of Biochemistry and Physiology. Rua Nelson Chaves S/N, Cidade Universitária, CEP 50670-420, Recife, PE, Brazil

    Biologist graduated from Faculdade Frassinetti do Recife (2007-2011). Master in Biochemistry and Physiology from the Federal University of Pernambuco (2011-2013). PhD in Biological Sciences from the Federal University of Pernambuco (2013-2017).

    Carolina Barbosa Malafaia

    Center for Strategic Technologies of the Northeast, Avenida Professor Luiz Freire, 01, Cidade Universitária, CEP 50740-545, Recife, PE, Brazil.

    OrcID https://orcid.org/0000-0001-6482-5341

    Graduated in Biological Sciences/Bachelor's Degree from the Federal University of Pernambuco (2009), Master's in Post-Graduation in Biological Sciences from the Federal University of Pernambuco (2012) and PhD in Biological Sciences from the Federal University of Pernambuco (2016). He has experience in Biochemistry, with emphasis on Biochemistry, working mainly on the following topics: Biofilms, microbiology and biopolymer production.

    Paula Fernanda Figueiredo das Mercês

    Federal University of Pernambuco (UFPE), Department of Biochemistry and Physiology. Rua Nelson Chaves S/N, Cidade Universitária, CEP 50670-420, Recife, PE, Brazil.

    PhD and Master in Biological Sciences with emphasis on Biotechnology from the Federal University of Pernambuco (2013 -2019), I work in the area of prospection, isolation, purification and characterization of biomolecules from Caatinga plants, with potential for biotechnological applications, covering in particular oils essentials and organic extracts. Specialist in Environmental Planning and Management from the University of Pernambuco (2014-2015). Graduated in Biological Sciences at the University of Pernambuco (2008-2012). I am currently studying Biological Sciences Degree, as a pedagogical complementation, at the Federal Rural University of Pernambuco (UFRPE). She performs scientific consultancy as a content designer for teaching materials and ongoing work and ABNT standards.

    Daniel Rodrigo Cavalcante de Araújo

    National Institute of the Semiarid Region. Ministry of Science, Technology and Innovations. Avenida Francisco Lopes de Almeida Serrotão, CEP 58434-700, Campina Grande, PB, Brazil

    http://lattes.cnpq.br/1451153004498643

    Graduated in Environmental Sciences (2008-2012) from the Federal University of Pernambuco (UFPE), master (2013-2015) and doctor (2015-2018) in Biological Sciences, with emphasis on Biotechnology (PPGCB/UFPE), is familiar with operating in Preparative and analytical HPLC-QDa ® and UPLC-QTOF Masslynx software in order to isolate and identify active substances of plant secondary metabolism. He currently belongs to the Institutional Training Program of the Ministry of Science, Technology and Innovation (PCI / MCTI / CNPq), at the National Institute of the Semi-Arid (INSA / MCTI) where he works with bioprospecting / phytochemistry.

    Ana Paula Sant'Anna da Silva

    Federal University of Pernambuco (UFPE), Department of Biochemistry and Physiology. Rua Nelson Chaves S/N, Cidade Universitária, CEP 50670-420, Recife, PE, Brazil.

    I am currently a postdoctoral fellow at the Postgraduate Program in Biochemistry and Physiology, by the National Post-Doctoral Program (PNPD) of CAPES. Experience in Biological Sciences, with emphasis on Biochemistry, working mainly in the following areas: biotechnology, medicinal plants, microbiology and pharmacology. PhD in Biological Sciences at the Federal University of Pernambuco (2012 - 2016), Research line biotechnology, medicinal plants, microbiology and pharmacology. Master in Biological Sciences at the Federal University of Pernambuco (2010-2012). Graduation in Biological Sciences Degree from the Federal University of Pernambuco.

    Vera Lúcia de Menezes Lima

    Federal University of Pernambuco (UFPE), Department of Biochemistry and Physiology. Rua Nelson Chaves S/N, Cidade Universitária, CEP 50670-420, Recife, PE, Brazil.

    http://lattes.cnpq.br/8429792710135888

    He holds a degree in Biomedicine from the Federal University of Pernambuco (1973), a master's degree in Biochemistry and Physiology from the Federal University of Pernambuco (1979), a PhD in Biochemistry from the University of London (1989) and a postdoctoral degree from the University of London (1993). He is currently a full professor at the Federal University of Pernambuco, Reviewer of the Brazilian Journal of Medical and Biological Research (0100-879X), Reviewer of the promotion project of the National Council for Scientific and Technological Development, Reviewer of the promotion project of the Coordination of Improvement of Higher Education Personnel, Reviewer of a promotion project of the Fundação de Amparo à Pesquisa do Estado de São Paulo, Reviewer of a journal of Medicinal Chemistry Research (Print) and Collaborator without employment relationship at INSTITUTO AGGEU MAGALHÃ?ES FIOCRUZ/PE. He has experience in the field of Biochemistry, with an emphasis on Macromolecule Chemistry. Acting mainly on the following topics: LCAT, Lipids, Hep G2 cell line, Cell culture, Antibodies and Biotechnology.

    Fabiane Rabelo da Costa Batista

    National Institute of the Semiarid Region. Ministry of Science, Technology and Innovations

    Technical training in biotechnology from ETFQ-RJ/ CEFETEQ (1996), a degree in Agronomy from the Federal University of Viçosa (2002), a master's degree in Plant Production from the State University of Norte Fluminense Darcy Ribeiro (2004) and a PhD in Genetics and Plant Breeding from the same university (2008), with a sandwich period at Texas A&M University, USA. He has experience in Agronomy, with emphasis on Genetics, working mainly on the following topics: cytogenetics, plant genetic resources and molecular markers. She is currently a Researcher in the area of Plant Biodiversity at the Instituto Nacional do Semiárido-INSA, in Campina Grande, PB.

    Cláudia Sampaio de Andrade Lima

    Federal University of Pernambuco (UFPE), Department of Biochemistry and Physiology. Rua Nelson Chaves S/N, Cidade Universitária, CEP 50670-420, Recife, PE, Brazil.

    Full Professor at the Federal University of Pernambuco. She is the President Director of the Technological Institute of Biosustainable Chains (ITCBio), Coordinator of the Juá Caatinga Pole of the Biodiversity Route of the Ministry of Regional Development (MDR) of Brazil and Coordinator of the Technical Committee on Medicinal Plants and Phytotherapics (CTFITO) of Pernambuco. She is part of REDESFITO do Brasil, working in the Brazilian semi-arid region. She has experience in the field of Chemistry and Pharmacy, working on the following subjects: Bioactives from the flora and microbiota of the Northeastern semi-arid region, for therapeutic and cosmetic purposes. She is leader of the Biotechnology of Natural Products Research Group (BIONAT). It is part of the Structuring Group of Biocosmetics and Natural Actives from Amazonia. She is a Permanent Member of the Graduate Program in Morphotechnology (PPGM), level 4 of CAPES. Ad-hoc advisor for the Brazilian Journal of Pharmacognosy, Journal of Integrative Medicine, Brazilian Journal of Pharmaceutical Science, Acta Amazônica and Revista Brasileira de Plantas Medicinais.

    Elineide Barbosa de Souza

    Federal Rural University of Pernambuco (UFRPE), Department of Biology, Microbiology Area. Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, CEP 52171-900, Recife, PE, Brazil.

    He holds a degree in Agronomy from the Federal Rural University of Pernambuco (1991), a master's degree in Plant Health from the Federal Rural University of Pernambuco (1995) and a PhD in Phytopathology from the Federal Rural University of Pernambuco (2002). She is a Full Professor at the Department of Biology at the Federal Rural University of Pernambuco, and works in the Postgraduate Program in Phytopathology of this Institution. She has experience in Phytopathology, with an emphasis on Phytobacteriology, working mainly in the following lines of research: ecology and characterization of phytopathogens, biological control of plant diseases. CNPq productivity scholarship level 1D.

    Maria Tereza dos Santos Correia

    Federal University of Pernambuco, Department of Biochemistry, Department of Biochemistry, Laboratory of Glycoproteins. Avenida Professor Moraes Rego S/N, Cidade Universitária, CEP 50670-910, Recife, PE, Brazil.

    He holds a degree in Industrial Chemistry from the Federal University of Pernambuco (1980), a Master's degree in Biochemistry from the Federal University of Pernambuco (1989) and a PhD in Biological Sciences (Molecular Biology) from the Federal University of São Paulo (1995). He is currently a Full Professor at the Federal University of Pernambuco and a Research Productivity Scholar at CNPq-Level 1C. She acted as coordinator and vice-coordinator of the Graduate Program in Biological Sciences (PPGCB-UFPE), vice-coordinator of the Masters in Biochemistry at UFPE, Head and Deputy Head of the Department of Biochemistry; in different periods. She also served as General Coordinator of the Scientific Initiation Program at UFPE (PIBIC, PIBIC-AF, PIBIC-EM and PIBIT), from January 2016 to November 2019. She was a member of the Advisory Committee in Biochemistry, Biophysics, Pharmacology, Physiology and Neurosciences - BF - CNPq (November 2015 to June 2018) and the Advisory and Evaluation Chamber of the Biological Sciences area, FACEPE (2015 to November 2019). She works in the undergraduate course of Biomedicine (UFPE) and as Permanent Professor at PPGCB, at the Postgraduate Program in Biochemistry and Physiology (PPGBF) and at the Postgraduate Program in Biotechnology (RENORBIO). Editor and Reviewer of international journals. She supervised more than 40 Scientific Initiation students, 70 Masters and 40 Doctors and supervised 8 Post-Doctors. Currently, she advises 7 undergraduate students (2 PIBIC, 2 PIBIT and 1 PIBIC_EM from the UFPE / CNPq Program and 2 BIC from the FACEPE Program), 6 master's students, 18 doctoral students and 2 post-docs. She has published 220 articles in impact journals, 23 book chapters and 2 books. Member of the Brazilian Society of Biochemistry and Molecular Biology. Member of the Brazilian Society for the Progress of Science and Member of the Pernambuco Academy of Sciences. Has experience in the field of Biochemistry of macromolecules with emphasis on proteins, essential oils and molecules of secondary metabolism of plants, acting on the following subjects: purification, characterization and biological applications of proteins (plants and animals), secondary metabolites and essential oils of plants. 

    Maria Luiza Vilela Oliva

    Federal University of São Paulo, Department of Biochemistry, Rua Três de Maio, 100, Vila Clementino, CEP 04044-020, São Paulo, SP, Brazil.

    Graduated in Licentiate and Bachelor's Degree in Chemistry - Faculdades Oswaldo Cruz (1978), Master's degree in Biological Sciences (Molecular Biology) from the Federal University of São Paulo (1981) and a PhD in Biological Sciences (Molecular Biology) from the Federal University of São Paulo (1988). ). He is currently Full Professor and Associate Professor at the Federal University of São Paulo. Researcher 1A at CNPq. Works with the characterization of the structural and kinetic aspects of inhibitors of proteolytic enzymes isolated from plants, their interaction with enzymes to understand the phenomena involved in the control of proteolysis and that may allow the design of inhibitors that interfere in biological processes such as blood clotting , blood pressure control, inflammation and tumor cell proliferation. In his Lattes curriculum, the most frequent terms in the context of scientific, technological and artistic-cultural production are: platelet aggregation; Bauhinia; protease inhibitors; cancer; breast cancer; coagulation; protein structure; inflammation; kallikrein-kinin system; Kunitz; medicinal plants; active peptides; thrombosis.

Keywords

Angico
Xanthomonas campestris
Acidovorax citrulli
HPLC-DAD
Flash Chromatography
HPLC-preparative

Abstract

Several microorganisms are responsible for great economic losses in world agriculture. Preventive and treatment methods are applied to avoid contamination of crops by these microorganisms, however, the use of chemical antimicrobials damages health and the environment. Secondary plant metabolites are safe natural sources of antimicrobials for this application. Fabaceae family has its history described in the literature as a potential source for obtaining antimicrobial bioactive. The objective of this work was to isolate bioactive compounds guided by antimicrobial assays against bacteria and fungi in vitro. Organic extracts were prepared by eluotropic series of leaves of Anadenanthera colubrina var. cebil and were tested against six bacteria and six fungi phytopathogenic. The antimicrobial assays of minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) were performed at each purification step that occurred through HPLC-DAD, Flash Chromatography and HPLC-preparative analysis, to confirm the isolation of the bioactive. Through bioguided isolation, the compound p-hydroxybenzoic acid was obtained, which showed activity against the phytobacteria Xanthomonas campestris pv. campestris and Acidovorax citrulli.

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Author(s)

  • Cibele Maria Alves da Silva-Bessa
    Federal University of Pernambuco (UFPE), Department of Biochemistry and Physiology. Rua Nelson Chaves S/N, Cidade Universitária, CEP 50670-420, Recife, PE, Brazil
  • Carolina Barbosa Malafaia
    Center for Strategic Technologies of the Northeast, Avenida Professor Luiz Freire, 01, Cidade Universitária, CEP 50740-545, Recife, PE, Brazil.
    https://orcid.org/0000-0001-6482-5341
  • Paula Fernanda Figueiredo das Mercês
    Federal University of Pernambuco (UFPE), Department of Biochemistry and Physiology. Rua Nelson Chaves S/N, Cidade Universitária, CEP 50670-420, Recife, PE, Brazil.
  • Daniel Rodrigo Cavalcante de Araújo
    National Institute of the Semiarid Region. Ministry of Science, Technology and Innovations. Avenida Francisco Lopes de Almeida Serrotão, CEP 58434-700, Campina Grande, PB, Brazil
  • Ana Paula Sant'Anna da Silva
    Federal University of Pernambuco (UFPE), Department of Biochemistry and Physiology. Rua Nelson Chaves S/N, Cidade Universitária, CEP 50670-420, Recife, PE, Brazil.
  • Vera Lúcia de Menezes Lima
    Federal University of Pernambuco (UFPE), Department of Biochemistry and Physiology. Rua Nelson Chaves S/N, Cidade Universitária, CEP 50670-420, Recife, PE, Brazil.
  • Fabiane Rabelo da Costa Batista
    National Institute of the Semiarid Region. Ministry of Science, Technology and Innovations
  • Cláudia Sampaio de Andrade Lima
    Federal University of Pernambuco (UFPE), Department of Biochemistry and Physiology. Rua Nelson Chaves S/N, Cidade Universitária, CEP 50670-420, Recife, PE, Brazil.
  • Elineide Barbosa de Souza
    Federal Rural University of Pernambuco (UFRPE), Department of Biology, Microbiology Area. Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, CEP 52171-900, Recife, PE, Brazil.
  • Maria Tereza dos Santos Correia
    Federal University of Pernambuco, Department of Biochemistry, Department of Biochemistry, Laboratory of Glycoproteins. Avenida Professor Moraes Rego S/N, Cidade Universitária, CEP 50670-910, Recife, PE, Brazil.
  • Maria Luiza Vilela Oliva
    Federal University of São Paulo, Department of Biochemistry, Rua Três de Maio, 100, Vila Clementino, CEP 04044-020, São Paulo, SP, Brazil.

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Isolation of active antiphytopathogen compound from extracts of Anadenanthera colubrina var. cebil (Griseb.) Altschul. Rev Fitos [Internet]. 2022 Sep. 30 [cited 2024 Nov. 15];16(3):324-3. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1265
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