Variação das propriedades físico-químicas de extratos de Bidens pilosa L. Asteraceae (picão preto) influenciada pelo processo extrativo

Júlio Cézar Borella; Bianca de Fátima Bredariol  Oliveira

doi:10.32712/2446-4775.2022.1353

Variation of the physico-chemical properties of extracts of Bidens pilosa L. Asteraceae (hairy beggartick) influenced by the extractive process

Vol. 17 No. 1 (2023)
103-111
14/10/2021
31/03/2023
https://doi.org/10.32712/2446-4775.2022.1353

Júlio Cézar Borella
Bianca de Fátima Bredariol Oliveira

Keywords

Phytotherapy

Phytotherapeutic Drugs

Plant Extracts

Abstract

In order to provide the basis for the introduction of a larger number of plant species and their derivatives in the national pharmaceutical compendia, physicochemical, chromatographic and quantitative analyzes of the marker were performed in extracts obtained from Bidens pilosa L. Asteraceae (hairy beggartick) aerial parts, species with antioxidant, antibacterial, anti-inflammatory, antitumor, antimalarial, immunomodulatory, hepatoprotective, healing activities, among others. From hydroethanolic (EtOH: H₂O 77:23), hydroglycolic (PEG:H₂O 1:1) and aqueous extracts obtained by maceration (1:10), the density, pH, solid content, chromatographic profile (TLC) and total flavonoid content (spectrophotometry) were evaluated. The analyzes showed that the highest density extracts were aqueous (0.9595 g / mL) and hydroglycolic (1.0418 g / mL). Hydroglycolic (pH = 6.32) and hydroethanolic (pH = 6.29) extracts showed more acids than aqueous ones (pH = 6.95). Higher dry residues were presented by hydroethanolic (2.40%) and hydroglycolic (2.70%) extracts compared to aqueous (0.53%). Quercetin was identified by TLC in all extracts tested and greater diversity of substances was evidenced in hydroethanolic and hydroglycolic extracts. Higher flavonoid contents were found in hydroethanolic and hydroglycolic extracts (0.009%; 0.008% respectively) than in aqueous extracts (0.003%). In spite of this, the aqueous extracts are more selectively extracted for the researched marker.

References

Nemitz M, Mallmann L, Steppe M. Evolução dos métodos quantitativos empregados para plantas medicinais ao longo das edições da farmacopeia brasileira. Rev Eletr Farm. [online]. 2016. 13(1): 18-27. [acesso em: 16 out. 2021]. [https://doi.org/10.5216/ref.v13i1.31789].
Oliveira MDT, Rodrigues E, Casu L, Benítez G, Leonti M. The historical development of pharmacopoeias and the inclusion of exotic herbal drugs with a focus on Europe and Brazil. J Ethnopharmacol. [online]. 2019. 240: 111891. [acesso em: 16 out. de 2021]. [https://doi.org/10.1016/j.jep.2019.111891].
Souza-Moreira TM, Salgado HRN, Pietro RCRL. O Brasil no contexto de controle de qualidade de plantas medicinais. Rev Bras Farmacogn [online]. 2010; 20(3): 435-440. [acesso em: 16 out. 2021]. [https://doi.org/10.1590/S0102-695X2010000300023].
Brasil. Ministério da Saúde. Departamento de Assistência Farmacêutica e Insumos Estratégicos. Monografia da espécie Bidens pilosa (Picão – preto). 73p. Brasília; 2015. [acesso em: 16 out. 2021]. [http://portalarquivos.saude.gov.br/images/pdf/2017/setembro/11/Monografia-Bidens.pdf].
Gilbert B, Alves LF, Favoreto R. Bidens pilosa L. Asteraceae (Compositae; subfamília Heliantheae). Rev Fitos. [online]. 2014; 8(1): 53-67. [acesso em: 16 out. 2021]. [https://doi.org/10.32712/2446-4775.2013.194].
Xuan TD, Khanh TD. Chemistry and pharmacology of Bidens pilosa: an overview. J Pharm Investig [online]. 2016. 46(2): 91-132. [acesso em: 16 out. 2021]. [https://doi.org/10.1007/s40005-016-0231-6].
Corren J, Lemay M, Lin Y, Rozga L, Randolph RK. Clinical and biochemical effects of a combination botanical product (ClearGuardâ„¢) for allergy: a pilot randomized double-blind placebo-controlled trial. Nutrition J [online]. 2008. 7(1): 20. [acesso em: 16 out. 2021]. [https://doi.org/10.1186/1475-2891-7-20].
Degelo GC. Estudo do efeito do extrato hidroglicólico de Bidens pilosa na expressão de genes relacionados à integridade da pele. 67p. Botucatu. 2010. Dissertação de Mestrado [Programa de Pós-graduação em Ciências Biológicas] – UNESP, Botucatu. 2010. [acesso em: 16 out. 2021]. [https://repositorio.unesp.br/bitstream/handle/11449/92438/degelo_gc_me_botib.pdf?sequence=1&isAllowed=y]
Ereno D. Beleza retocada. Rev Pesq Fapesp. [online]. 2011. 190: 70-5. [acesso em: 16 out. 2021]. [https://revistapesquisa.fapesp.br/beleza-retocada/].
Horiuchi M, Seyama Y. Improvement of the anti-inflammatory and anti-allergic activity of Bidens pilosa L. var. radiata Scherff treated with enzyme (cellulosine). J health Sci. [online]. 2008. 54(3): 294-301. [acesso em: 16 out. 2021]. [https://doi.org/10.1248/jhs.54.294].
Yang WC, Chang LT, Liang YC. - inventores; depositante. Beneficial effect of Bidens pilosa on fat decrease and muscle increase. U.S. Patente no. 16/068,125, 3 jan. 2019; [acesso em: 16 out. 2021]. [https://patentimages.storage.googleapis.com/fb/3c/7e/d8c162b51e19d8/US20190000904A1.pdf].
Bozinis MCV, Lima EM, Batista AC, Maretto RN, De Mendonça EF. - inventores; depositante. Soluble, stable, anti-inflammatory, proliferative, protective and mucoadhesive pharmaceutical compositions; use thereof for treating mucositis conditions and method for producing same; base pharmaceutical composition for preparing the pharmaceutical compositions and method for producing same. U.S. Patente no 15/522, 763, de 9 nov. 2017. [acesso em: 16 out. 2021]. [https://patentimages.storage.googleapis.com/2c/09/0c/9b1d82e8f6a3d4/US20170319642A1.pdf].
Brasil. Ministério da Saúde. Agência Nacional de Vigilância Sanitária. Farmacopeia Brasileira. 6ª ed. Volume I e II. Brasília; 2019 [acesso em: 16 out. 2021]. [https://www.gov.br/anvisa/pt-br/assuntos/farmacopeia/farmacopeia-brasileira].
Borella JC, Borella PH, Gastaldi MD, Miranda CES. Bidens pilosa - picão preto: influência da adubação orgânica e da luminosidade na produtividade e no teor de flavonoides. Rev Fitos. [online]. 2019; 13(4): 261-269. [acesso em: 16 out. 2021]; [https://doi.org/10.32712/2446-4775.2019.761].
Borella JC, Fontoura A. Avaliação do perfil cromatográfico e do teor de flavonoides em amostras de Baccharis trimera Less DC., Asteraceae (carqueja) comercializadas em Ribeirão Preto, SP, Brasil. Rev Bras Farmacogn. [online]. 2002. 12(2): 63-67. [acesso em: 16 out. 2021]. [https://doi.org/10.1590/S0102-695X2002000200002].
Singh G, Passari AK, Singh P, Leo VV, Subbarayan S, Kumar B et al. Pharmacological potential of Bidens pilosa L. and determination of bioactive compounds using UHPLC-QqQ LIT-MS/MS and GC/MS. BMC Complement Med Ther. [Online]. 2017. 17(1): 492-508. [acesso em: 16 out. 2021]. [https://doi.org/10.1186/s12906-017-2000-0].
Silva FL. Contribuição à farmacognosia de Artemisia annua L. e Bidens pilosa L. (Asteraceae). Acompanhamento da variação de metabólitos secundários em diferentes fases fenológicas, órgãos e extratos vegetais, aspectos botânicos e avaliação da atividade antileishmania in vitro. 236p. São Paulo, 2008. Dissertação de Mestrado [Programa de Pós-Graduação em Fármacos e Medicamentos] - Universidade de São Paulo, USP, São Paulo, 2008. [acesso em: 16 out. 2021]. [https://teses.usp.br/teses/disponiveis/9/9138/tde-18092017-143745/en.php].
Cortés‐Rojas DF, Souza CRF, Oliveira WP. Optimisation of the extraction of phenolic compounds and antioxidant activity from aerial parts of Bidens pilosa L. using response surface methodology. Int J Food Sci Technol [online]. 2011; 46(11): 2420-2427. [acesso em: 16 out. 2021]. [https://doi.org/10.1111/j.1365-2621.2011.02765.x].
Cortés-Rojas DF, Chagas-Paula DA, Da Costa FB, Souza CR, Oliveira WP. Bioactive compounds in Bidens pilosa L. populations: a key step in the standardization of phytopharmaceutical preparations. Rev Bras Farmacogn [online]. 2013; 23(1): 28-35. [acesso em: 16 out. 2021]. [https://doi.org/10.1590/S0102-695X2012005000100].
Cortés-Rojas DF, Souza CRF, Oliveira WP. Assessment of stability of a spray dried extract from the medicinal plant Bidens pilosa L. J King Saud Univ. [online]. 2016 28(2): 141-146. [acesso em: 16 out. 2021]. [https://doi.org/10.1016/j.jksues.2014.04.004].

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Author(s)

Júlio Cézar Borella
Centro Universitário Barão de Mauá
http://orcid.org/0000-0003-3782-9851
Bianca de Fátima Bredariol Oliveira
Centro Universitário Barão de Mauá
http://orcid.org/0000-0002-7983-0108

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Variation of the physico-chemical properties of extracts of Bidens pilosa L. Asteraceae (hairy beggartick) influenced by the extractive process. Rev Fitos [Internet]. 2023 Mar. 31 [cited 2025 Apr. 8];17(1):103-11. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1353

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10.5965/223811712342024581

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] Nemitz M, Mallmann L, Steppe M. Evolução dos métodos quantitativos empregados para plantas medicinais ao longo das edições da farmacopeia brasileira. Rev Eletr Farm. [online]. 2016. 13(1): 18-27. [acesso em: 16 out. 2021]. [https://doi.org/10.5216/ref.v13i1.31789].

[2] Oliveira MDT, Rodrigues E, Casu L, Benítez G, Leonti M. The historical development of pharmacopoeias and the inclusion of exotic herbal drugs with a focus on Europe and Brazil. J Ethnopharmacol. [online]. 2019. 240: 111891. [acesso em: 16 out. de 2021]. [https://doi.org/10.1016/j.jep.2019.111891].

[3] Souza-Moreira TM, Salgado HRN, Pietro RCRL. O Brasil no contexto de controle de qualidade de plantas medicinais. Rev Bras Farmacogn [online]. 2010; 20(3): 435-440. [acesso em: 16 out. 2021]. [https://doi.org/10.1590/S0102-695X2010000300023].

[4] Brasil. Ministério da Saúde. Departamento de Assistência Farmacêutica e Insumos Estratégicos. Monografia da espécie Bidens pilosa (Picão – preto). 73p. Brasília; 2015. [acesso em: 16 out. 2021]. [http://portalarquivos.saude.gov.br/images/pdf/2017/setembro/11/Monografia-Bidens.pdf].

[5] Gilbert B, Alves LF, Favoreto R. Bidens pilosa L. Asteraceae (Compositae; subfamília Heliantheae). Rev Fitos. [online]. 2014; 8(1): 53-67. [acesso em: 16 out. 2021]. [https://doi.org/10.32712/2446-4775.2013.194].

[6] Xuan TD, Khanh TD. Chemistry and pharmacology of Bidens pilosa: an overview. J Pharm Investig [online]. 2016. 46(2): 91-132. [acesso em: 16 out. 2021]. [https://doi.org/10.1007/s40005-016-0231-6].

[7] Corren J, Lemay M, Lin Y, Rozga L, Randolph RK. Clinical and biochemical effects of a combination botanical product (ClearGuardâ„¢) for allergy: a pilot randomized double-blind placebo-controlled trial. Nutrition J [online]. 2008. 7(1): 20. [acesso em: 16 out. 2021]. [https://doi.org/10.1186/1475-2891-7-20].

[8] Degelo GC. Estudo do efeito do extrato hidroglicólico de Bidens pilosa na expressão de genes relacionados à integridade da pele. 67p. Botucatu. 2010. Dissertação de Mestrado [Programa de Pós-graduação em Ciências Biológicas] – UNESP, Botucatu. 2010. [acesso em: 16 out. 2021]. [https://repositorio.unesp.br/bitstream/handle/11449/92438/degelo_gc_me_botib.pdf?sequence=1&isAllowed=y]

[9] Ereno D. Beleza retocada. Rev Pesq Fapesp. [online]. 2011. 190: 70-5. [acesso em: 16 out. 2021]. [https://revistapesquisa.fapesp.br/beleza-retocada/].

[10] Horiuchi M, Seyama Y. Improvement of the anti-inflammatory and anti-allergic activity of Bidens pilosa L. var. radiata Scherff treated with enzyme (cellulosine). J health Sci. [online]. 2008. 54(3): 294-301. [acesso em: 16 out. 2021]. [https://doi.org/10.1248/jhs.54.294].

[11] Yang WC, Chang LT, Liang YC. - inventores; depositante. Beneficial effect of Bidens pilosa on fat decrease and muscle increase. U.S. Patente no. 16/068,125, 3 jan. 2019; [acesso em: 16 out. 2021]. [https://patentimages.storage.googleapis.com/fb/3c/7e/d8c162b51e19d8/US20190000904A1.pdf].

[12] Bozinis MCV, Lima EM, Batista AC, Maretto RN, De Mendonça EF. - inventores; depositante. Soluble, stable, anti-inflammatory, proliferative, protective and mucoadhesive pharmaceutical compositions; use thereof for treating mucositis conditions and method for producing same; base pharmaceutical composition for preparing the pharmaceutical compositions and method for producing same. U.S. Patente no 15/522, 763, de 9 nov. 2017. [acesso em: 16 out. 2021]. [https://patentimages.storage.googleapis.com/2c/09/0c/9b1d82e8f6a3d4/US20170319642A1.pdf].

[13] Brasil. Ministério da Saúde. Agência Nacional de Vigilância Sanitária. Farmacopeia Brasileira. 6ª ed. Volume I e II. Brasília; 2019 [acesso em: 16 out. 2021]. [https://www.gov.br/anvisa/pt-br/assuntos/farmacopeia/farmacopeia-brasileira].

[14] Borella JC, Borella PH, Gastaldi MD, Miranda CES. Bidens pilosa - picão preto: influência da adubação orgânica e da luminosidade na produtividade e no teor de flavonoides. Rev Fitos. [online]. 2019; 13(4): 261-269. [acesso em: 16 out. 2021]; [https://doi.org/10.32712/2446-4775.2019.761].

[15] Borella JC, Fontoura A. Avaliação do perfil cromatográfico e do teor de flavonoides em amostras de Baccharis trimera Less DC., Asteraceae (carqueja) comercializadas em Ribeirão Preto, SP, Brasil. Rev Bras Farmacogn. [online]. 2002. 12(2): 63-67. [acesso em: 16 out. 2021]. [https://doi.org/10.1590/S0102-695X2002000200002].

[16] Singh G, Passari AK, Singh P, Leo VV, Subbarayan S, Kumar B et al. Pharmacological potential of Bidens pilosa L. and determination of bioactive compounds using UHPLC-QqQ LIT-MS/MS and GC/MS. BMC Complement Med Ther. [Online]. 2017. 17(1): 492-508. [acesso em: 16 out. 2021]. [https://doi.org/10.1186/s12906-017-2000-0].

[17] Silva FL. Contribuição à farmacognosia de Artemisia annua L. e Bidens pilosa L. (Asteraceae). Acompanhamento da variação de metabólitos secundários em diferentes fases fenológicas, órgãos e extratos vegetais, aspectos botânicos e avaliação da atividade antileishmania in vitro. 236p. São Paulo, 2008. Dissertação de Mestrado [Programa de Pós-Graduação em Fármacos e Medicamentos] - Universidade de São Paulo, USP, São Paulo, 2008. [acesso em: 16 out. 2021]. [https://teses.usp.br/teses/disponiveis/9/9138/tde-18092017-143745/en.php].

[18] Cortés‐Rojas DF, Souza CRF, Oliveira WP. Optimisation of the extraction of phenolic compounds and antioxidant activity from aerial parts of Bidens pilosa L. using response surface methodology. Int J Food Sci Technol [online]. 2011; 46(11): 2420-2427. [acesso em: 16 out. 2021]. [https://doi.org/10.1111/j.1365-2621.2011.02765.x].

[19] Cortés-Rojas DF, Chagas-Paula DA, Da Costa FB, Souza CR, Oliveira WP. Bioactive compounds in Bidens pilosa L. populations: a key step in the standardization of phytopharmaceutical preparations. Rev Bras Farmacogn [online]. 2013; 23(1): 28-35. [acesso em: 16 out. 2021]. [https://doi.org/10.1590/S0102-695X2012005000100].

[20] Cortés-Rojas DF, Souza CRF, Oliveira WP. Assessment of stability of a spray dried extract from the medicinal plant Bidens pilosa L. J King Saud Univ. [online]. 2016 28(2): 141-146. [acesso em: 16 out. 2021]. [https://doi.org/10.1016/j.jksues.2014.04.004].