Total polyphenols from Aloysia gratissima and bioavailability after subchronic oral treatment in Wistar rats
Dr. Gênifer Erminda Schreiner
https://orcid.org/0000-0002-0203-3944
PhD candidate in the Postgraduate Program in Biochemistry at the Federal University of Pampa since 2022, master in Biochemistry (2022), member of the Research Group on Oxidative Stress (GESTOX), CAPES scholarship holder. Graduated from the Biological Sciences course - Degree at the Federal University of Fronteira Sul, Campus Cerro Largo (2020). He received a scholarship from the Multidisciplinary Pedagogical Residency Program (CAPES) and the Institutional Teaching Initiation Scholarship Program (CAPES), he worked as a volunteer monitor in the area of Molecular Biology and in the Research Project on Histochemical, Genotoxic, Isolation and Structural Analysis of Natural Products obtained from medicinal plants of the Verbenaceae family native to Rio Grande do Sul (FAPERGS).
Mrs. Elizandra Gomes Schmitt
https://orcid.org/0009-0007-9813-5542
Master's student in the Postgraduate Program in Biochemistry (2022). Pharmacist, graduated from the Federal University of Pampa (UNIPAMPA) Campos Uruguaiana- RS. Member of the Oxidative Stress Study Group (GESTOX), she has experience in the areas of Hematology, Oxidative Stress and Animal Experimentation.
Teacher Laura Smolski dos Santos
https://orcid.org/0000-0001-8858-9654
Pharmacist, CRF 583735, graduated from the Federal University of Pampa (UNIPAMPA), Uruguaiana campus - RS. Member of the Oxidative Stress Study Group (GESTOX), focusing on the areas of: Hematology and oxidative stress. Master's degree from the Postgraduate Program in Biochemistry at UNIPAMPA, studying the relationship between oxidative markers and COVID-19 in individuals after a two-dose vaccination schedule. Currently, a doctoral candidate in the Postgraduate Program in Biochemistry at UNIPAMPA, CAPES scholarship holder.
Drª Dr. Silvia Muller de Moura Sarmento
https://orcid.org/0000-0002-6872-406X
Biomedical, Clinical Pathologist since 2008, and mother of 8-year-old Martin. PhD in Physiology from PPGMCF UNIPAMPA (2023). Master in Health Sciences with an emphasis on Pathogenesis and Pathophysiology from the Federal University of Health Sciences of Porto Alegre - UFCSPA (2012), Specialist in Clinical and Molecular Analysis from the Academy of Science and Technology, in São José do Rio Preto/SP (2009 ), and Specialist in Field Epidemiology - FIOCRUZ/BSB (2021). Currently coordinator of the Border Laboratory of Uruguaiana and Biomedicine of the Municipal Laboratory of Clinical Analysis of the Municipal Health Department of Uruguaiana. Passionate about life and science, knowledge feeds the soul and transmitting what you know comforts the heart.
Mrs. Luana Tamires Maders
https://orcid.org/0009-0008-2948-7023
Graduate of the Pharmacy course at the Federal University of Pampa (UNIPAMPA), Uruguaiana campus - RS. Scientific initiation student and member of the Oxidative Stress Study Group (GESTOX), focusing on the areas of: Hematology, Oxidative Stress and Animal Experimentation. Member of the extension project entitled "Heelfoot test: valuing your baby's life". He worked as an Academic Management Support Fellow at the Federal University of Pampa.
Mrs. Gabriela Escalante Brites
https://orcid.org/0009-0004-9877-5951
Graduate of the Pharmacy course at the Federal University of Pampa (UNIPAMPA), Uruguaiana campus - RS. Scientific initiation student and member of the Oxidative Stress Study Group (GESTOX), focusing on the areas of: Hematology, Oxidative Stress and Animal Experimentation. Member of the extension project entitled "Heelfoot test: valuing your baby's life". He worked as an Academic Management Support Fellow at the Federal University of Pampa.
Mrs. Camila Berny Pereira
https://orcid.org/0009-0000-0023-0306
Student of the Pharmacy Course at the Federal University of Pampa 2021, member of the Research Group on Oxidative Stress (GESTOX), PDA scholarship holder, works on the group's research projects on the following topics: obesity, liver disease, oxidative stress and moro orange. Participant in the Rational Disposal of Medicines extension project.
Dr. Rafael Tamborena Malheiros
https://orcid.org/0000-0002-6163-3122
* Doctor and Master in Physiological Sciences from the Multicenter Postgraduate Program in Physiological Sciences of the Brazilian Society of Physiology (Associated Institution: Unipampa). Specialist in Intensive Care with a postgraduate degree from Faculdade Inspirar. Physiotherapist graduated from the Federal University of Pampa (Unipampa) Uruguaiana campus. * Coordinator of the Physiotherapist Service at Hospital Santa Casa de Uruguaiana (HSCCU), being Technical Responsible for the Adult Intensive Care Units, working in the coordination of hospitalization units. Health Director at the Ilha da Esperança Social Project in the community of Bairro Mascaranhas de Moraes (Marduque) in Uruguaiana.* During graduation, scholarship holder of the Tutorial Education Program (PET) of the Ministry of Education, through the University Extension Program for Integrated Practices in Health Collective (PISC), from UNIPAMPA. Intern (scholarship) at the HSCCU Physiotherapy Service.
Dr. Nessana Dartora
https://orcid.org/0000-0001-7700-0904
He holds a degree in Biological Sciences from the Integrated Regional University of Alto Uruguai and Missões (2008), a master's and doctorate in Sciences (Biochemistry) from the Federal University of Paraná (2014). She is currently an Adjunct Professor at the Federal University of Fronteira Sul, Campus Cerro Largo. She has experience in the area of Biochemistry, with an emphasis on Glycoconjugate Chemistry and their biological activities, working mainly on the following topics: Structural Analysis of Secondary Metabolites, Analysis by GC-MS, ESI-MS, HPLC-MS, NMR and Anti- inflammatory (sepsis).
Dr. Vanusa Manfredini
https://orcid.org/0000-0002-9622-7861
Mother of Bernardo and Rafael. She has a Research Productivity Grant from CNPq (PQ2). She has a degree in Pharmacy, emphasis in Clinical Analysis from the Federal University of Rio Grande do Sul (2003), a master's degree (2004) in Cellular and Molecular Biology and a PhD in Sciences from the Federal University of Rio Grande do Sul (2008). Post-doctorate in Pharmaceutical Sciences from UFRGS (20210). She worked as a teacher at the Integrated Regional University (URI-Campus de Erechim) (2005-2010). She is currently Associate Professor 3 at the Federal University of Pampa (UNIPAMPA), Campus Uruguaiana. She served as coordinator of the PPGCS Specialization Course in Health Sciences (2012-2014). She was coordinator of the Ethics Committee on the Use of Animals (CEUA-UNIPAMPA) (2015-2020) and coordinator of the Pharmacy Course (2021-2023). She served as coordinator/tutor in the Intervention area of PET Occupational Health (2022-2023). It has projects in three areas: teaching, research and extension. In the teaching area, in addition to the curricular components she teaches alongside the Pharmacy course, she has a teaching project focusing on the development of a Digital Atlas as a learning tool for students on health courses. She works as a project and extension coordinator on topics such as Heel Foot Test, Viral Hepatitis and Human Papilloma Virus (HPV) Infection with the local community. She is a master's and doctorate advisor in the Postgraduate Program in Biochemistry (UNIPAMPA). She works as a reviewer for several international journals. She is leader of the Oxidative Stress Study Group (GESTOX) where she guides scientific initiation students, course completion work and postgraduate students. GESTOX's main line of research is to understand the role of functional foods and/or supplements in the prevention of chronic-metabolic diseases and neuro-degenerative diseases. She has experience in the areas of hematology, clinical biochemistry, cytopathology, oxidative stress, pain and inflammation.
Keywords
Abstract
The present work carried out the quantification of total polyphenols in the aqueous extract of the leaves of Aloysia gratissima, and its oral absorption index in Wistar rats. For this, 20 adult rats were divided into five treatment groups: saline solution; 100 mg/Kg of A. gratissima extract; 50 mg/Kg and 100 mg/Kg of rutin and co-treatment with 50 mg/Kg of extract and 10 mg/Kg of rutin, performed orally for 14 days. For the quantification of total polyphenols, the Folin Ciocalteau method was used. In the extract, the concentration of total polyphenols was 7.11 mg GAE/mL, in the plasma of control animals the baseline level was 4.3 mg GAE/mL. In the treated groups, significantly higher values were observed, of 9.32 mg GAE/mL, 6.65 mg GAE/mL, 8.34 mg GAE/mL and 10.14 mg GAE/mL, respectively. Such data configure A. gratissima as a good source for obtaining total polyphenols due both to its high concentration in the extract and also to their good bioavailability.
References
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Author(s)
-
Dr. Gênifer Erminda Schreiner
Federal University of Pampa (Unipampa)
https://orcid.org/0000-0002-0203-3944
-
Mrs. Elizandra Gomes Schmitt
Federal University of Pampa (Unipampa)
https://orcid.org/0009-0007-9813-5542
-
Teacher Laura Smolski dos Santos
Federal University of Pampa (Unipampa)
https://orcid.org/0000-0001-8858-9654
-
Drª Dr. Silvia Muller de Moura Sarmento
Federal University of Pampa (Unipampa)
https://orcid.org/0000-0002-6872-406X
-
Mrs. Luana Tamires Maders
Federal University of Pampa (Unipampa)
https://orcid.org/0009-0008-2948-7023
-
Mrs. Gabriela Escalante Brites
Federal University of Pampa (Unipampa)
https://orcid.org/0009-0004-9877-5951
-
Mrs. Camila Berny Pereira
Federal University of Pampa (Unipampa)
https://orcid.org/0009-0000-0023-0306
-
Dr. Rafael Tamborena Malheiros
Hospital Santa Casa de Caridade de Uruguaiana
https://orcid.org/0000-0002-6163-3122
-
Dr. Nessana Dartora
Federal University of Fronteira SUL (UFFS)
https://orcid.org/0000-0001-7700-0904
-
Dr. Vanusa Manfredini
Federal University of Pampa (Unipampa)
https://orcid.org/0000-0002-9622-7861
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