Uso da Cromatografia Contracorrente na obtenção de padrões de origem Vegetal

G. G. Leitão

doi:10.32712/2446-4775.2005.23

Use of Countercurrent Chromatography for the preparation of standard compounds from plants

Vol. 1 No. 02 (2005)
48-50
11/10/2013
30/11/2005
https://doi.org/10.32712/2446-4775.2005.23

G. G. Leitão

Keywords

Coutercurrent Chromatography

standards

natural products

Abstract

Countercurrent chromatography technique is presented here as a tool for the fast, efficient and economic preparation of highly pure standard compounds from plant origin. Some aspects concerning solvent systems choice as well as examples of the isolation and purification of pure natural compounds of pharmaceutical importance, such as flavonoids from Ginkgo biloba, saponins from Centella asiatica and Ginseng, anthraquinones, etc., are discussed.

References

ALVI, K.A. Screening Natural Products: Bioassay-Directed Isolation of Active Compounds by Dual-Mode CCC. Journal of Chromatography and Related Technologies, v.24, n.11&12, p.1765-1773, 2001.
BERTHOD, A. Practical Approach to High-Speed Counter-current Chromatography. Journal of Chromatography, v.550, p677-693, 1991.
CAO, X.; TIAN, Y.; ZHANG, T.; LIU, Q.; JIA, L.; ITO, Y. Separation of Dammarane-Saponins from Notoginseng, Root of Panax notoginseng (Burk.) F. H. Chen, by HSCCC Coupled with Evaporative Light Scattering Detector. Journal of Chromatography and Related Technologies, v.26, n.9&10, p.1579-1591, 2003.
CONWAY, W.D. Counter-Current Chromatography: Apparatus, Theory and Applications. New York: VCH Publishers, Inc., 1990.
DIALLO, B.; VANHAELEN-FASTRE, R.; VANHAELEN, M. Direct-Coupling of High-Speed Counter-current Chromatography to Thin-layer Chromatography. Application to the Separation of Asiaticoside and Madecassoside from Centella asiatica. J. Chrom., v.556, p446-450, 1991.
FOUCAULT, A.P. Solvent Systems in Centrifugal Partition Chromatography In Centrifugal Partition Chromatography. New York: ed.Chromatographic Science Series, e.1; Alain P. Foucault, Marcel Dekker Inc., v.68, p.90-92., 1995.
GARRAD, I. Simple Approach to the Development of a CCC Solvent Selection Protocol Suitable for Automation. Journal of Chromatography and Related Technologies, v.28, n.12-13, p.1923-1935, 2005.
HOSTETTMANN, K.; MARSTON, A. Countercurrent Chromatography in the Preparative Separation of Plant-Derived Natural Products. Journal of Chromatography and Related Technologies, v.24, n.11-12, p.1711-1721, 2001.
HOSTETTMANN, K.; HOSTETTMANN, M.; MARSTON, A. Isolation of Natural Products by Droplet Counter-current Chromatography and Related methods. Natural Products Report, v.1, n.5, p.409-512, 1984.
LEITÃO, G.G.; EL-ADJI, S.S.; DE MELO, W.L.; LEITÃO, S.G.; BROWN, L. Separation of Free and Glycosylated Flavonoids from Siparuna guianensis by Gradient and Isocratic CCC. Journal of Chromatography and Related Technologies, v.28, n.12-13, p.2041-2051, 2005.
LEITÃO, G.G.; DE SOUZA, P.A.; MORAES, A.A.; BROWN, L. Step-Gradient CCC Separation of Phenylpropanoids and Iridoid Glycosides from Roots of Stachytarpheta cayennensis (Rich.) Vahl. Journal of Chromatography and Related Technologies, v.28, n.12-13, p.2053-2060, 2005.
MARSTON, A.; SLACANIN, I.; HOSTETTMANN, K.Centrifugal Partition Chromatography in the Separation of natural Products. Phytochemical Analysis, v.1, p3-17, 1990.
MARSTON, A.; HOSTETTMANN, K. Counter-current Chromatography as a Preparative Tool - Applications and Perspectives. Journal of Chromatography, v.658, p.315-341, 1994.
MIKELL, J.R.; SCHANEBERG, B.T.; KHAN, I.A. Isolation and Purification of Kava lactones by High Performance Centrifugal Partition Chromatography. Journal of Chromatography and Related Technologies, v.26, n.18, p.3069-3074, 2003.
RENAULT, J.H., NUZZILLARD, J.M.; INTES, O.; MACIUK, A. Solvent Systems In: BERTHOD, A. (Ed.) Countercurrent Chromatography, The Support Free Liquid Stationary Phase. New York: Elsevier, e.1, Comprehemsive Analytical Chemistry, Vol. XXXVIII, 2002.
SCHÃ„FER, K.; WINTERHALTER, P. Application of High Speed Countercurrent Chromatography (HSCCC) to the Isolation of Kavalactones, Journal of Chromatography and Related Technologies, v.28, n.11, p1703-1716, 2005.
ZHANG, T. Separation and Purification of natural Products (Medicinal Herbs) by High Speed Countercurrent Chromatography In: BERTHOD, A. (Ed.) Countercurrent Chromatography, The Support
Free Liquid Stationary Phase. New York: Elsevier, e.1, Comprehemsive Analytical Chemistry, v. XXXVIII, 2002.
ZHANG, T.; CAO, X.; HAN, X. Preparation of National Certified Reference Materials of Active Compounds from Natural Products by CCC. Journal of Chromatography and Related Technologies, v.26, n.9-10, 2003.
YUA, L. M.; CHEN, X. X.; AI, P.; ZI, M.; WU, P.; LI, Z. Y. Versatile Two-Phase Solvent System for Anthraquinone Prefractionation by High Speed Countercurrent Chromatography. Journal of Chromatography and Related Technologies, v.24, n.19, p.2961-1970, 2001.

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Author(s)

G. G. Leitão
Núcleo de Pesquisas de Produtos Naturais Universidade Federal do Rio de Janeiro, Bloco H, CCS, Ilha do Fundão, 21941-590 Rio de Janeiro, RJ, Brasil

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Use of Countercurrent Chromatography for the preparation of standard compounds from plants. Rev Fitos [Internet]. 2005 Nov. 30 [cited 2025 Dec. 5];1(02):48-50. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/23

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] ALVI, K.A. Screening Natural Products: Bioassay-Directed Isolation of Active Compounds by Dual-Mode CCC. Journal of Chromatography and Related Technologies, v.24, n.11&12, p.1765-1773, 2001.

[2] BERTHOD, A. Practical Approach to High-Speed Counter-current Chromatography. Journal of Chromatography, v.550, p677-693, 1991.

[3] CAO, X.; TIAN, Y.; ZHANG, T.; LIU, Q.; JIA, L.; ITO, Y. Separation of Dammarane-Saponins from Notoginseng, Root of Panax notoginseng (Burk.) F. H. Chen, by HSCCC Coupled with Evaporative Light Scattering Detector. Journal of Chromatography and Related Technologies, v.26, n.9&10, p.1579-1591, 2003.

[4] CONWAY, W.D. Counter-Current Chromatography: Apparatus, Theory and Applications. New York: VCH Publishers, Inc., 1990.

[5] DIALLO, B.; VANHAELEN-FASTRE, R.; VANHAELEN, M. Direct-Coupling of High-Speed Counter-current Chromatography to Thin-layer Chromatography. Application to the Separation of Asiaticoside and Madecassoside from Centella asiatica. J. Chrom., v.556, p446-450, 1991.

[6] FOUCAULT, A.P. Solvent Systems in Centrifugal Partition Chromatography In Centrifugal Partition Chromatography. New York: ed.Chromatographic Science Series, e.1; Alain P. Foucault, Marcel Dekker Inc., v.68, p.90-92., 1995.

[7] GARRAD, I. Simple Approach to the Development of a CCC Solvent Selection Protocol Suitable for Automation. Journal of Chromatography and Related Technologies, v.28, n.12-13, p.1923-1935, 2005.

[8] HOSTETTMANN, K.; MARSTON, A. Countercurrent Chromatography in the Preparative Separation of Plant-Derived Natural Products. Journal of Chromatography and Related Technologies, v.24, n.11-12, p.1711-1721, 2001.

[9] HOSTETTMANN, K.; HOSTETTMANN, M.; MARSTON, A. Isolation of Natural Products by Droplet Counter-current Chromatography and Related methods. Natural Products Report, v.1, n.5, p.409-512, 1984.

[10] LEITÃO, G.G.; EL-ADJI, S.S.; DE MELO, W.L.; LEITÃO, S.G.; BROWN, L. Separation of Free and Glycosylated Flavonoids from Siparuna guianensis by Gradient and Isocratic CCC. Journal of Chromatography and Related Technologies, v.28, n.12-13, p.2041-2051, 2005.

[11] LEITÃO, G.G.; DE SOUZA, P.A.; MORAES, A.A.; BROWN, L. Step-Gradient CCC Separation of Phenylpropanoids and Iridoid Glycosides from Roots of Stachytarpheta cayennensis (Rich.) Vahl. Journal of Chromatography and Related Technologies, v.28, n.12-13, p.2053-2060, 2005.

[12] MARSTON, A.; SLACANIN, I.; HOSTETTMANN, K.Centrifugal Partition Chromatography in the Separation of natural Products. Phytochemical Analysis, v.1, p3-17, 1990.

[13] MARSTON, A.; HOSTETTMANN, K. Counter-current Chromatography as a Preparative Tool - Applications and Perspectives. Journal of Chromatography, v.658, p.315-341, 1994.

[14] MIKELL, J.R.; SCHANEBERG, B.T.; KHAN, I.A. Isolation and Purification of Kava lactones by High Performance Centrifugal Partition Chromatography. Journal of Chromatography and Related Technologies, v.26, n.18, p.3069-3074, 2003.

[15] RENAULT, J.H., NUZZILLARD, J.M.; INTES, O.; MACIUK, A. Solvent Systems In: BERTHOD, A. (Ed.) Countercurrent Chromatography, The Support Free Liquid Stationary Phase. New York: Elsevier, e.1, Comprehemsive Analytical Chemistry, Vol. XXXVIII, 2002.

[16] SCHÃ„FER, K.; WINTERHALTER, P. Application of High Speed Countercurrent Chromatography (HSCCC) to the Isolation of Kavalactones, Journal of Chromatography and Related Technologies, v.28, n.11, p1703-1716, 2005.

[17] ZHANG, T. Separation and Purification of natural Products (Medicinal Herbs) by High Speed Countercurrent Chromatography In: BERTHOD, A. (Ed.) Countercurrent Chromatography, The Support

[18] Free Liquid Stationary Phase. New York: Elsevier, e.1, Comprehemsive Analytical Chemistry, v. XXXVIII, 2002.

[19] ZHANG, T.; CAO, X.; HAN, X. Preparation of National Certified Reference Materials of Active Compounds from Natural Products by CCC. Journal of Chromatography and Related Technologies, v.26, n.9-10, 2003.

[20] YUA, L. M.; CHEN, X. X.; AI, P.; ZI, M.; WU, P.; LI, Z. Y. Versatile Two-Phase Solvent System for Anthraquinone Prefractionation by High Speed Countercurrent Chromatography. Journal of Chromatography and Related Technologies, v.24, n.19, p.2961-1970, 2001.