Uso da Cromatografia Contracorrente na obtenção de padrões de origem Vegetal

G. G. Leitão

doi:10.32712/2446-4775.2005.23

Uso da Cromatografia Contracorrente na obtenção de padrões de origem Vegetal

v. 1 n. 02 (2005)
48-50
11/10/2013
30/11/2005
https://doi.org/10.32712/2446-4775.2005.23

G. G. Leitão

Palavras-chave

Cromatografia contracorrente

padrÃµes

produtos naturais

Resumo

A técnica da cromatografia contracorrente é aqui apresentada como uma ferramenta eficiente, rápida e econômica de obtenção de padrões de origem vegetal com alto grau de pureza. São discutidos alguns aspectos da escolha dos sistemas de solventes adequados além de exemplos de isolamento e purificação de substâncias naturais de importância farmacêutica tais como flavonóides de Ginkgo biloba, saponinas de Centella asiatica e Ginseng, antraquinonas, etc.

Referências

ALVI, K.A. Screening Natural Products: Bioassay-Directed Isolation of Active Compounds by Dual-Mode CCC. Journal of Chromatography and Related Technologies, v.24, n.11&12, p.1765-1773, 2001.
BERTHOD, A. Practical Approach to High-Speed Counter-current Chromatography. Journal of Chromatography, v.550, p677-693, 1991.
CAO, X.; TIAN, Y.; ZHANG, T.; LIU, Q.; JIA, L.; ITO, Y. Separation of Dammarane-Saponins from Notoginseng, Root of Panax notoginseng (Burk.) F. H. Chen, by HSCCC Coupled with Evaporative Light Scattering Detector. Journal of Chromatography and Related Technologies, v.26, n.9&10, p.1579-1591, 2003.
CONWAY, W.D. Counter-Current Chromatography: Apparatus, Theory and Applications. New York: VCH Publishers, Inc., 1990.
DIALLO, B.; VANHAELEN-FASTRE, R.; VANHAELEN, M. Direct-Coupling of High-Speed Counter-current Chromatography to Thin-layer Chromatography. Application to the Separation of Asiaticoside and Madecassoside from Centella asiatica. J. Chrom., v.556, p446-450, 1991.
FOUCAULT, A.P. Solvent Systems in Centrifugal Partition Chromatography In Centrifugal Partition Chromatography. New York: ed.Chromatographic Science Series, e.1; Alain P. Foucault, Marcel Dekker Inc., v.68, p.90-92., 1995.
GARRAD, I. Simple Approach to the Development of a CCC Solvent Selection Protocol Suitable for Automation. Journal of Chromatography and Related Technologies, v.28, n.12-13, p.1923-1935, 2005.
HOSTETTMANN, K.; MARSTON, A. Countercurrent Chromatography in the Preparative Separation of Plant-Derived Natural Products. Journal of Chromatography and Related Technologies, v.24, n.11-12, p.1711-1721, 2001.
HOSTETTMANN, K.; HOSTETTMANN, M.; MARSTON, A. Isolation of Natural Products by Droplet Counter-current Chromatography and Related methods. Natural Products Report, v.1, n.5, p.409-512, 1984.
LEITÃO, G.G.; EL-ADJI, S.S.; DE MELO, W.L.; LEITÃO, S.G.; BROWN, L. Separation of Free and Glycosylated Flavonoids from Siparuna guianensis by Gradient and Isocratic CCC. Journal of Chromatography and Related Technologies, v.28, n.12-13, p.2041-2051, 2005.
LEITÃO, G.G.; DE SOUZA, P.A.; MORAES, A.A.; BROWN, L. Step-Gradient CCC Separation of Phenylpropanoids and Iridoid Glycosides from Roots of Stachytarpheta cayennensis (Rich.) Vahl. Journal of Chromatography and Related Technologies, v.28, n.12-13, p.2053-2060, 2005.
MARSTON, A.; SLACANIN, I.; HOSTETTMANN, K.Centrifugal Partition Chromatography in the Separation of natural Products. Phytochemical Analysis, v.1, p3-17, 1990.
MARSTON, A.; HOSTETTMANN, K. Counter-current Chromatography as a Preparative Tool - Applications and Perspectives. Journal of Chromatography, v.658, p.315-341, 1994.
MIKELL, J.R.; SCHANEBERG, B.T.; KHAN, I.A. Isolation and Purification of Kava lactones by High Performance Centrifugal Partition Chromatography. Journal of Chromatography and Related Technologies, v.26, n.18, p.3069-3074, 2003.
RENAULT, J.H., NUZZILLARD, J.M.; INTES, O.; MACIUK, A. Solvent Systems In: BERTHOD, A. (Ed.) Countercurrent Chromatography, The Support Free Liquid Stationary Phase. New York: Elsevier, e.1, Comprehemsive Analytical Chemistry, Vol. XXXVIII, 2002.
SCHÃ„FER, K.; WINTERHALTER, P. Application of High Speed Countercurrent Chromatography (HSCCC) to the Isolation of Kavalactones, Journal of Chromatography and Related Technologies, v.28, n.11, p1703-1716, 2005.
ZHANG, T. Separation and Purification of natural Products (Medicinal Herbs) by High Speed Countercurrent Chromatography In: BERTHOD, A. (Ed.) Countercurrent Chromatography, The Support
Free Liquid Stationary Phase. New York: Elsevier, e.1, Comprehemsive Analytical Chemistry, v. XXXVIII, 2002.
ZHANG, T.; CAO, X.; HAN, X. Preparation of National Certified Reference Materials of Active Compounds from Natural Products by CCC. Journal of Chromatography and Related Technologies, v.26, n.9-10, 2003.
YUA, L. M.; CHEN, X. X.; AI, P.; ZI, M.; WU, P.; LI, Z. Y. Versatile Two-Phase Solvent System for Anthraquinone Prefractionation by High Speed Countercurrent Chromatography. Journal of Chromatography and Related Technologies, v.24, n.19, p.2961-1970, 2001.

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G. G. Leitão
Núcleo de Pesquisas de Produtos Naturais Universidade Federal do Rio de Janeiro, Bloco H, CCS, Ilha do Fundão, 21941-590 Rio de Janeiro, RJ, Brasil

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Uso da Cromatografia Contracorrente na obtenção de padrões de origem Vegetal. Rev Fitos [Internet]. 30º de novembro de 2005 [citado 17º de novembro de 2024];1(02):48-50. Disponível em: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/23

Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.

[1] ALVI, K.A. Screening Natural Products: Bioassay-Directed Isolation of Active Compounds by Dual-Mode CCC. Journal of Chromatography and Related Technologies, v.24, n.11&12, p.1765-1773, 2001.

[2] BERTHOD, A. Practical Approach to High-Speed Counter-current Chromatography. Journal of Chromatography, v.550, p677-693, 1991.

[3] CAO, X.; TIAN, Y.; ZHANG, T.; LIU, Q.; JIA, L.; ITO, Y. Separation of Dammarane-Saponins from Notoginseng, Root of Panax notoginseng (Burk.) F. H. Chen, by HSCCC Coupled with Evaporative Light Scattering Detector. Journal of Chromatography and Related Technologies, v.26, n.9&10, p.1579-1591, 2003.

[4] CONWAY, W.D. Counter-Current Chromatography: Apparatus, Theory and Applications. New York: VCH Publishers, Inc., 1990.

[5] DIALLO, B.; VANHAELEN-FASTRE, R.; VANHAELEN, M. Direct-Coupling of High-Speed Counter-current Chromatography to Thin-layer Chromatography. Application to the Separation of Asiaticoside and Madecassoside from Centella asiatica. J. Chrom., v.556, p446-450, 1991.

[6] FOUCAULT, A.P. Solvent Systems in Centrifugal Partition Chromatography In Centrifugal Partition Chromatography. New York: ed.Chromatographic Science Series, e.1; Alain P. Foucault, Marcel Dekker Inc., v.68, p.90-92., 1995.

[7] GARRAD, I. Simple Approach to the Development of a CCC Solvent Selection Protocol Suitable for Automation. Journal of Chromatography and Related Technologies, v.28, n.12-13, p.1923-1935, 2005.

[8] HOSTETTMANN, K.; MARSTON, A. Countercurrent Chromatography in the Preparative Separation of Plant-Derived Natural Products. Journal of Chromatography and Related Technologies, v.24, n.11-12, p.1711-1721, 2001.

[9] HOSTETTMANN, K.; HOSTETTMANN, M.; MARSTON, A. Isolation of Natural Products by Droplet Counter-current Chromatography and Related methods. Natural Products Report, v.1, n.5, p.409-512, 1984.

[10] LEITÃO, G.G.; EL-ADJI, S.S.; DE MELO, W.L.; LEITÃO, S.G.; BROWN, L. Separation of Free and Glycosylated Flavonoids from Siparuna guianensis by Gradient and Isocratic CCC. Journal of Chromatography and Related Technologies, v.28, n.12-13, p.2041-2051, 2005.

[11] LEITÃO, G.G.; DE SOUZA, P.A.; MORAES, A.A.; BROWN, L. Step-Gradient CCC Separation of Phenylpropanoids and Iridoid Glycosides from Roots of Stachytarpheta cayennensis (Rich.) Vahl. Journal of Chromatography and Related Technologies, v.28, n.12-13, p.2053-2060, 2005.

[12] MARSTON, A.; SLACANIN, I.; HOSTETTMANN, K.Centrifugal Partition Chromatography in the Separation of natural Products. Phytochemical Analysis, v.1, p3-17, 1990.

[13] MARSTON, A.; HOSTETTMANN, K. Counter-current Chromatography as a Preparative Tool - Applications and Perspectives. Journal of Chromatography, v.658, p.315-341, 1994.

[14] MIKELL, J.R.; SCHANEBERG, B.T.; KHAN, I.A. Isolation and Purification of Kava lactones by High Performance Centrifugal Partition Chromatography. Journal of Chromatography and Related Technologies, v.26, n.18, p.3069-3074, 2003.

[15] RENAULT, J.H., NUZZILLARD, J.M.; INTES, O.; MACIUK, A. Solvent Systems In: BERTHOD, A. (Ed.) Countercurrent Chromatography, The Support Free Liquid Stationary Phase. New York: Elsevier, e.1, Comprehemsive Analytical Chemistry, Vol. XXXVIII, 2002.

[16] SCHÃ„FER, K.; WINTERHALTER, P. Application of High Speed Countercurrent Chromatography (HSCCC) to the Isolation of Kavalactones, Journal of Chromatography and Related Technologies, v.28, n.11, p1703-1716, 2005.

[17] ZHANG, T. Separation and Purification of natural Products (Medicinal Herbs) by High Speed Countercurrent Chromatography In: BERTHOD, A. (Ed.) Countercurrent Chromatography, The Support

[18] Free Liquid Stationary Phase. New York: Elsevier, e.1, Comprehemsive Analytical Chemistry, v. XXXVIII, 2002.

[19] ZHANG, T.; CAO, X.; HAN, X. Preparation of National Certified Reference Materials of Active Compounds from Natural Products by CCC. Journal of Chromatography and Related Technologies, v.26, n.9-10, 2003.

[20] YUA, L. M.; CHEN, X. X.; AI, P.; ZI, M.; WU, P.; LI, Z. Y. Versatile Two-Phase Solvent System for Anthraquinone Prefractionation by High Speed Countercurrent Chromatography. Journal of Chromatography and Related Technologies, v.24, n.19, p.2961-1970, 2001.