Determinação de Guaco em Mel por Cromatografia Líquida de Alta Eficiência

M. C. Marcucci; S. C. Caparica

doi:10.32712/2446-4775.2006.53

Determination of Guaco in Honey by High Perfomance Liquid Chromatography

Vol. 2 No. 02 (2006)
54-57
16/10/2013
01/09/2006
https://doi.org/10.32712/2446-4775.2006.53

M. C. Marcucci
S. C. Caparica

Keywords

Abstract

The guaco (Mikania glomerata) is a plant originated from Brazil that is known by its expectorant properties. The Department of Inspection of Products from Animal Origin, linked to the Agricultural Ministry of Brazil, has made a resolution on 19/06/2003, related to honey that have vegetal extracts aggregated to it. Also, the Sanitary Vigilance National Agency of Brazil (ANVISA) has made a public consult in 06/11/03 regarding to the regulation of products for phytotherapeutic products. These considerations take into account the necessity of to elaborate legal rules and legal analytic procedures based on scientific literature. These considerations led us to develop a methodology to detect the coumarin in honey that has guaco aggregated to it. The method uses a High Performance Liquid Chromatography with diode-array detector equipment. This method may be a reliable qualitative method to detect and quantify the guaco coumarin in commercial honey claiming to have Mikania glomerata plant extract in them.

References

CELEGHINI, R.M.S.; VILEGAS, J.H.Y.; LANCAS, F.M. Extraction and Quantitative HPLC Analysis of Coumarin in Hydroalcoholic Extracts of Mikania glomerata Spreng: ("guaco") Leaves. Journal of the Brazilian Chemical Society, v.12, n.6, p.706-709, 2002.
CORRÊA, P. Dicionário das Plantas Úteis do Brasil e das Exóticas Cultivadas, 2a ed., Ed. Imprensa Nacional, Ministério da Agricultura: Rio de Janeiro; 1984.
DIPOA: Departamento de Inspeção de Produtos de Origem Animal, Ministério da Agricultura, Portaria n.o 11 de 19/06/2003.
FARMACOPÉIA: Farmacopéia Brasileira. 4a ed. São Paulo: Atheneu, 1988-1996.

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Author(s)

M. C. Marcucci
Laboratório de Produtos Naturais do Programa de Pós-graduação em Farmácia da Universidade Bandeirante de São Paulo, UNIBAN Rua Maria Cândida, 1813, CEP-02071-013, Vila Guilherme São Paulo, SP, Brasil
S. C. Caparica
Natural Labor - Análises e Pesquisas Ltda. Rua Lauro Vanucci, 1020, Jardim Santa Cândida, 13087-548 Campinas, SP, Brasil

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How to Cite

Determination of Guaco in Honey by High Perfomance Liquid Chromatography. Rev Fitos [Internet]. 2006 Sep. 1 [cited 2025 Feb. 21];2(02):54-7. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/53

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] CELEGHINI, R.M.S.; VILEGAS, J.H.Y.; LANCAS, F.M. Extraction and Quantitative HPLC Analysis of Coumarin in Hydroalcoholic Extracts of Mikania glomerata Spreng: ("guaco") Leaves. Journal of the Brazilian Chemical Society, v.12, n.6, p.706-709, 2002.

[2] CORRÊA, P. Dicionário das Plantas Úteis do Brasil e das Exóticas Cultivadas, 2a ed., Ed. Imprensa Nacional, Ministério da Agricultura: Rio de Janeiro; 1984.

[3] DIPOA: Departamento de Inspeção de Produtos de Origem Animal, Ministério da Agricultura, Portaria n.o 11 de 19/06/2003.

[4] FARMACOPÉIA: Farmacopéia Brasileira. 4a ed. São Paulo: Atheneu, 1988-1996.