Plant therapeutic proteases: chemical aspects, applications and pharmaceutical formulation

Raquel Elisa Silva-López
OrcID
Patrícia Fernandes Ferreira
OrcID

    Raquel Elisa Silva-López

    Oswaldo Cruz Foundation, Farmanguinhos. Department of Natural Products. Avenida Brasil, 4365, Manguinhos, CEP 21045-900, Rio de Janeiro, RJ, Brazil.

    OrcID https://orcid.org/0000-0003-2744-7884

    Tropical Parasitic Diseases at the Universitat de Valencia in Spain. She completed a postgraduate degree in Clinical Research at Hospital Alemão -SUS. She works at FIOCRUZ as a researcher in the Department of Natural Products at the Farmanguinhos unit. She is leader of the Biochemistry of Proteases and Protease Inhibitors of Natural Origin Group. She coordinates and teaches the Biochemistry and Biological Drugs subjects of the Technical Course in Biotechnology at IOC-FIOCRUZ. She has experience in the area of Biochemistry, with an emphasis on Biochemistry of microorganisms and plants, working mainly on the following topics: characterization and purification of proteins, enzymes, protease inhibitors, Leishmania and legumes.

    Patrícia Fernandes Ferreira

    Oswaldo Cruz Foundation, Farmanguinhos. Department of Natural Products. Avenida Brasil, 4365, Manguinhos, CEP 21045-900, Rio de Janeiro, RJ, Brazil.

    OrcID https://orcid.org/0000-0001-9640-930X

    He has a master's degree in Chemical and Biochemical Process Technology from the School of Chemistry at UFRJ (2010), a degree in Biological Science: in Microbiology and Immunology from the Federal University of Rio de Janeiro (2008) and a degree and bachelor's degree in Biological Sciences from Fundação Técnico Educacional Souza Marques - FTESM (1996). He has experience in the areas of Biochemistry, Microbiology, Immunology, Biotechnology, Bioprocesses, Chemical Synthesis and Purification of natural extracts, working mainly on the following topics: Pdr5p, marine sponges, bioleaching, biosynthetic production processes of recombinant human insulin, bioinsecticide, test development diagnostics, chemical synthesis of dendrimeric peptides and research into new phytotherapeutic targets of biological origin, with specificity on a chromagrography column. From 2009 to 2013 she worked at the Bioproducts Laboratory of Farmanguinhos/FIOCRUZ as a researcher in the technology transfer process for the production of recombinant human insulin in the agreement between the company INDAR and Farmanguinhos/FIOCRUZ, as well as in the agreement for the transfer of technology for the production of bioinsecticide developed by Farmanguinhos/FIOCRUZ for the company BR3 (2011/2012). From 2015 she was part of the CDTS / Fiocruz research and development group for diagnostic tests aimed at meeting public health demands. She is currently part of the technical team at the Farmanguinhos Natural Products Laboratory / Fiocruz, aiming at the research and development of biopharmaceuticals linked to neglected diseases.

Keywords

Plant enzymes
Therapeutic protease
Immobilization
Formulation

Abstract

Proteases are enzymes that cleave peptide bonds in proteins and peptides. Are found in all living organisms and mediate many crucial functions. These enzymes have been used by man since antiquity for various purposes such as food processing and treatment of various diseases. Plants are important sources of proteases with significant activity and high thermal stability and in the presence of various chemical agents. Such requirements are essential to your pharmacological employment. Many such as papain, bromelain and phytin are marketed to treat cases of respiratory and digestive insufficiency, parasitic infections and wound healing. Therapeutic proteases are very specific and efficient; however, they have very complex structures, and their maintenance requires very specific conditions to guarantee their biological / pharmacological activities. Therefore, their formulations are designed to stabilize their structure / activity, protect against degradation, improve their pharmacokinetics, prolong their action, reduce their toxic effects and direct them to their therapeutic target. Nanotechnology has made it possible to build drug carriers, including polymeric nano / microparticles: hydrogels, dendrimers, liposomes that are capable of increasing efficacy, clinical applicability and patient compliance. Such biodegradable / biocompatible polymers are promising carriers of these therapeutic plant proteases.

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Author(s)

  • Raquel Elisa Silva-López
    Oswaldo Cruz Foundation, Farmanguinhos. Department of Natural Products. Avenida Brasil, 4365, Manguinhos, CEP 21045-900, Rio de Janeiro, RJ, Brazil.
    https://orcid.org/0000-0003-2744-7884
  • Patrícia Fernandes Ferreira
    Oswaldo Cruz Foundation, Farmanguinhos. Department of Natural Products. Avenida Brasil, 4365, Manguinhos, CEP 21045-900, Rio de Janeiro, RJ, Brazil.
    https://orcid.org/0000-0001-9640-930X

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Plant therapeutic proteases: chemical aspects, applications and pharmaceutical formulation. Rev Fitos [Internet]. 2024 Mar. 14 [cited 2024 Nov. 21];18:e914. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/914
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