In vitro antileishmanial activity and phytochemical analysis of Casearia javitensis Kunth (Salicaceae)

Carolina Moraes da Silva
Luciana Guimarães Eiró
João Victor Silva-Silva
Juliana Corrêa-Barbosa
Márlia Regina Coelho-Ferreira
Fernando Tobias Silveira
Andrey Moacir do Rosário Marinho
Maria das Graças Carvalho Almeida
Heliton Patrick Cordovil Brigido
Maria Fâni Dolabela

    Carolina Moraes da Silva

    University Center of the State of Pará, Institute of Health Sciences, João Paulo do Valle Mendes Campus, Av. Almirante Barroso nº 3775, CEP: 6613-903. João Paulo do Valle Mendes, Belém, PA, Brazil.

    Graduated in Pharmacy from Centro Universitário do Estado do Pará (2017), served as a volunteer monitor of the discipline Histology and Embryology from 2013 to 2014 and as an official monitor in 2015, voluntarily integrated the project "Esse Rio é Minha Rua" - ENACTUS (2014 ). She was an intern at the Leishmaniasis Laboratory "Prof. Dr. Ralph Lainson", Parasitology Section, Evandro Chagas Institute / SVS / MS with incentives from the Institutional Program for Scientific Initiation Scholarships (PIBIC) 2014/2015. He concluded his master's degree in Pathology of Tropical Diseases by the postgraduate program in Tropical Medicine at the Federal University of Pará, in the session of Immunopathology of Tropical Diseases. She is currently a medical student in the fourth semester at Centro Universitário do Pará (CESUPA).

    Luciana Guimarães Eiró

    Federal University of Pará, Faculty of Pharmaceutical Sciences, Guamá University Campus, Rua Augusto Corrêa, 01, CEP: 66075-110, Belém, Pará, Brazil.

    He holds a BA in Pharmacy from the University Center of the State of Pará-CESUPA (2017). Worked as a volunteer monitor in the discipline of Cytology I (2013). Acted as a fellowship monitor in the Cytology discipline (2014). She worked as HR coordinator and Pharmacy sub-coordinator in the "Esse Rio é Minha Rua-Saúde" Project (2015) and as Pharmacy coordinator in the "Esse Rio é Minha Rua-Saúde" Project (2016). He started a postgraduate course in Pharmaceutical Care and Clinical Pharmacy at the DALMASS Institution in 2017. He worked at the Personale Manipulation Pharmacy from September to December 2017. Master's student of the Postgraduate Program in Pharmaceutical Sciences - PPGCF at the Federal University of Pará-UFPA , under the guidance of Prof. Dr. Rafael Rodrigues Lima.

    João Victor Silva-Silva

    Oswaldo Cruz Foundation - FIOCRUZ, Oswaldo Cruz Institute (IOC), Immunomodulation and Protozoology Laboratory, Av. Brasil, 4365, Manguinhos, CEP: 21040-360, Rio de Janeiro, RJ, Brazil

    Juliana Corrêa-Barbosa

    Federal University of Pará, Institute of Health Sciences, Guamá University Campus, Rua Augusto Corrêa, 01, CEP: 66075-110, Belém, Pará, Brazil.

    Pharmacist graduated from the Federal University of Pará (UFPA). Graduated from the PET-FARMÁCIA / UFPA group. Post-graduate student in Pharmaceutical Care and Clinical Pharmacy and Master's student in Pharmaceutical Sciences - PPGCF / UFPA.

    Márlia Regina Coelho-Ferreira

    Ministry of Science, Technology, Innovation and Communication, Coordination of Botany, Museu Paraense Emílio Goeldi, Avenida Governador Magalhães Barata, 376, São Brás, CEP: 66040-170, Belém, PA, Brazil.

    graduated in Pharmacy-Biochemistry from the Federal University of Ouro Preto (1986), master's degree in Biologie Végétale Tropicale - Universite de Paris VI (Pierre et Marie Curie) (1992) and doctorate in Biological Sciences from the Federal University of Pará (2000). She was a Full Researcher III at the Museu Paraense Emílio Goeldi (07/2000 - 02/2020) and is currently a researcher at the National Institute of the Atlantic Forest. He has experience in the field of Botany, with an emphasis on Ethnobotany, Ethnopharmacology and Economic Botany, working mainly in studies that address traditional knowledge of indigenous communities and peoples of the Brazilian Amazon on plant biodiversity, with emphasis on medicinal plants of cultural and economic importance. He teaches at the Postgraduate Programs in Biological Sciences - Tropical Plant Biology (UFRA / MPEG), at Rede Bionorte (Coordination State-Pará) and at the Post-Graduate Program in Sociocultural Diversity (MPEG).

    Fernando Tobias Silveira

    Ministry of Health, Health Surveillance Secretariat, Evandro Chagas Institute, Parasitology Department, Highway BR-316 KM 7 s/n, Levilândia, CEP: 67030-000, Ananindeua, PA, Brazil

    He holds a master's degree in Parasitology from the Federal University of Minas Gerais (1989), a doctorate in Pathology from the University of São Paulo (2001), a post-doctorate from the Faculty of Medicine of the University of São Paulo (2008), improvement in Parasite Genetics from the University of Cambridge (1995) and improvement in Parasite Genetics by the University of Cambridge (1997). He is currently a doctor-researcher at the Evandro Chagas Institute, PUBLIC HEALTH RESEARCHER at the Evandro Chagas Institute and Professor at the Federal University of Pará / Center for Tropical Medicine. Has experience in Medicine. Acting mainly on the following themes: Leishmaniasis cutaneous, Leishmania amazonensis, Pathogenesis.

    Andrey Moacir do Rosário Marinho

    Federal University of Pará, Institute of Exact and Natural Sciences, Faculty of Chemistry, Guamá University Campus, Rua Augusto Corrêa, 01, CEP: 66075-110, Belém, PA, Brazil

    Bachelor of Chemistry from the Federal University of Pará (2001), Master of Chemistry from the Federal University of São Carlos (2002) and Doctor of Chemistry from the Federal University of São Carlos (2005). He is currently Associate Professor I at the Federal University of Pará, based at the Institute of Exact and Natural Sciences - Faculty of Chemistry. Has experience in Chemistry of Natural Products and endophytic fungi associated with plants in the Amazon.

    Maria das Graças Carvalho Almeida

    Pará State University Center (CESUPA), Institute of Health Sciences, João Paulo do Valle Mendes Campus, Av. Almirante Barroso nº 3775, CEP: 6613-903, João Paulo do Valle Mendes, Belém, PA, Brazil.

    Graduated in Pharmacy and Biochemistry from the Federal University of Pará (1973), specialization in Public Health from the Ministry of Health / National School of Public Health and Federal University of Pará (1989); in the Public Health Laboratory with an emphasis on Bacteriology by the Instituto Adolfo Lutz (1979); in Distance Assessment by the University of Brasilia (1999); Master in Tropical Diseases by the Center for Tropical Medicine of the Federal University of Pará (2007). Currently as Assistant Professor IV of the Nursing Course at the University of the State of Pará and full professor of the Pharmacy Course at the Centro Universitário do Pará and former coordinator of the Clinical Analysis Laboratory of the Higher Education Center of Pará. With experience in the area of ​​Bacteriology with an emphasis on Public Health acquired by acting as Biochemical Pharmacist at LACEN, of the State Secretariat of Public Health of the State of Pará, acting mainly on the following themes: collective health and tropical diseases. (Text automatically generated by the CVLattes application)

    Heliton Patrick Cordovil Brigido

    Federal University of Pará, Institute of Health Sciences, Graduate Program in Pharmaceutical Innovation, Campus Universitário Guamá, Rua Augusto Corrêa, 01, CEP: 66075-110, Belém, PA, Brasil

    Graduated in Pharmacy (2014). Specialist in Clinical Pharmacy and Pharmaceutical Prescription (2016). Master's degree from the Postgraduate Program in Pharmaceutical Sciences from the Federal University of Pará (PPGCF, 2016), Area of Drugs and Medicines, Research line: Biological Evaluation of Natural and Synthetic Products. He is currently a PhD student at the Postgraduate Program in Pharmaceutical Innovation at the Federal University of Pará (PPGIF, 2017), Area of Drugs and Medicines, Research line: Planning, Research, Synthesis and Evaluation of Natural Products and Bioactive Molecules. Professor at the State University of Pará (UEPA) of the Medicine and Biomedicine courses and Professor at the Cosmopolitan Faculty teaching the subjects of Histology, Physiology, Pathological Processes and Pharmacology for courses in the health area.

    Maria Fâni Dolabela

    Federal University of Pará, Institute of Health Sciences, Postgraduate Program in Pharmaceutical Sciences, Rua Augusto Corrêa, 01, CEP: 66075-110, Belém, PA, Brazil

    Graduated in Pharmacy from the Federal University of Minas Gerais (1994), master's degree in Biological Sciences (Pharmacology) from the Federal University of Minas Gerais (1997), doctorate in Pharmaceutical Sciences from the Federal University of Minas Gerais (2007). Currently Associate Professor I at the Faculty of Pharmacy, Federal University of Pará, accredited to the Postgraduate Program in Pharmaceutical Sciences, UFPA (master's degree), Postgraduate Program in Pharmaceutical Innovation (doctorate) and coordinator of the Postgraduate Program in Health Care Pharmaceutical (Master-Professional). She also participates, as a tutor, in the group PET-Farmácia, UFPA and coordinates the Center for Information on Medicines of Pará. As a researcher she has been developing works in the areas of natural products, search for new drugs for neglected diseases and Pharmaceutical Assistance. Participates in the Board of the National School of Pharmacists.

Keywords

Leismaniasis
Antipromastigote
Cytotoxicity (THP-1)
Phenolic Compound

Abstract

The objective of this study was to carry out phytochemical analysis of the bark extract of Casearia javitensis and to evaluate its antipromastigote activity against Leishmania amazonensis. The ethanol extract (EE) was fractionated in open-column chromatography to obtain the hexane (FrHex), dichloromethane (FrDcm), ethyl acetate (FrAcOET) and methanol (FrMeOH) fractions. The EE and its fractions were analyzed by thin layer chromatography (TLC). FrDcm was analyzed in high performance liquid chromatography with diode array detection (HPLC-DAD). The antipromastigote assay of Leishmania amazonensis and the cytotoxicity test against the acute monocytic leukemia cell line (THP-1) were performed by the MTT colorimetric assay. The phytochemical profile in TLC detected terpenes in all samples. However, in the ultraviolet spectrum (HPLC-DAD) for FrDcm suggested the presence of a phenolic compound. FrHex and FrDcm showed antipromastigote activity (IC50 = 116.6 ± 0.9 and 59.4 ± 1.1 μg mL-1, respectively) and low cytotoxicity (CC50 = 333.4 ± 3.2 and 241.2 ± 1.9 μg mL-1, respectively). The selectivity index for FrDcm was 4.1. We conclude that the FrDcm of C. javitensis has potantial as a leishmanicide, and this activity may be related to the presence of phenolic compound.

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Author(s)

  • Carolina Moraes da Silva
    University Center of the State of Pará, Institute of Health Sciences, João Paulo do Valle Mendes Campus, Av. Almirante Barroso nº 3775, CEP: 6613-903. João Paulo do Valle Mendes, Belém, PA, Brazil.
  • Luciana Guimarães Eiró
    Federal University of Pará, Faculty of Pharmaceutical Sciences, Guamá University Campus, Rua Augusto Corrêa, 01, CEP: 66075-110, Belém, Pará, Brazil.
  • João Victor Silva-Silva
    Oswaldo Cruz Foundation - FIOCRUZ, Oswaldo Cruz Institute (IOC), Immunomodulation and Protozoology Laboratory, Av. Brasil, 4365, Manguinhos, CEP: 21040-360, Rio de Janeiro, RJ, Brazil
  • Juliana Corrêa-Barbosa
    Federal University of Pará, Institute of Health Sciences, Guamá University Campus, Rua Augusto Corrêa, 01, CEP: 66075-110, Belém, Pará, Brazil.
  • Márlia Regina Coelho-Ferreira
    Ministry of Science, Technology, Innovation and Communication, Coordination of Botany, Museu Paraense Emílio Goeldi, Avenida Governador Magalhães Barata, 376, São Brás, CEP: 66040-170, Belém, PA, Brazil.
  • Fernando Tobias Silveira
    Ministry of Health, Health Surveillance Secretariat, Evandro Chagas Institute, Parasitology Department, Highway BR-316 KM 7 s/n, Levilândia, CEP: 67030-000, Ananindeua, PA, Brazil
  • Andrey Moacir do Rosário Marinho
    Federal University of Pará, Institute of Exact and Natural Sciences, Faculty of Chemistry, Guamá University Campus, Rua Augusto Corrêa, 01, CEP: 66075-110, Belém, PA, Brazil
  • Maria das Graças Carvalho Almeida
    Pará State University Center (CESUPA), Institute of Health Sciences, João Paulo do Valle Mendes Campus, Av. Almirante Barroso nº 3775, CEP: 6613-903, João Paulo do Valle Mendes, Belém, PA, Brazil.
  • Heliton Patrick Cordovil Brigido
    Federal University of Pará, Institute of Health Sciences, Graduate Program in Pharmaceutical Innovation, Campus Universitário Guamá, Rua Augusto Corrêa, 01, CEP: 66075-110, Belém, PA, Brasil
  • Maria Fâni Dolabela
    Federal University of Pará, Institute of Health Sciences, Postgraduate Program in Pharmaceutical Sciences, Rua Augusto Corrêa, 01, CEP: 66075-110, Belém, PA, Brazil

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In vitro antileishmanial activity and phytochemical analysis of Casearia javitensis Kunth (Salicaceae). Rev Fitos [Internet]. 2020 Dec. 18 [cited 2025 Dec. 19];14(4):528-37. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/950
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