1. Fundação Oswaldo Cruz – Fiocruz, Instituto de Tecnologia em Fármacos - Farmanguinhos, Plataforma Agroecológica de Fitomedicamentos - PAF, Laboratório de Química da Biodiversidade, Avenida Comandante Guaranys, 447, Jacarepaguá, CEP 22775-903, Rio de Janeiro, RJ, Brasil. 2. 2Universidade Federal Fluminense, Faculdade de Farmácia, Pós-Graduação e Ciências Aplicadas a Produtos para Saúde, Rua Mário Viana, 523, Santa Rosa, Cep: 24.241-000, Niterói, RJ, Brasil.
Graduated in Pharmacy from Universidade Estácio de Sá (2005) and in Chemistry from Faculdade de Humanidades Pedro II (1992). Master and PhD in Sciences Applied to Health Products from Universidade Federal Fluminense (2012 and 2017). Postdoctoral student in Pharmaceutical Innovation from the Federal University of Goiás. He is currently a Technologist in Public Health in Farmanguinhos, Fiocruz, at the Center for Biodiversity and Health Management (NGBS) and acts as Coordinator of the Agroecological Phytomedicines Platform and of the Technological Development and Innovation Sector . He has extensive experience in the area of Pharmaceutical Technology Development, currently working on the development of pharmaceutical formulations from plant species.
Bruno da Motta Lessa
1. Fundação Oswaldo Cruz – Fiocruz, Instituto de Tecnologia em Fármacos - Farmanguinhos, Plataforma Agroecológica de Fitomedicamentos - PAF, Laboratório de Química da Biodiversidade, Avenida Comandante Guaranys, 447, Jacarepaguá, CEP 22775-903, Rio de Janeiro, RJ, Brasil
High School (2nd degree) by the Federal Center for Technological Chemistry Education of Nilópolis-CEFETEQ / RJ, Brazil. He is currently a research technician at the Oswaldo Cruz Foundation. Has experience in Chemistry, with emphasis on Analytical Instrumentation, acting on the following subjects: analytical instrumentation, liquid, gas chromatography, HPTLC in addition to mass spectrometry.
Carlos Henrique Brasil Bizarri
1Fundação Oswaldo Cruz – Fiocruz, Instituto de Tecnologia em Fármacos - Farmanguinhos, Plataforma Agroecológica de Fitomedicamentos - PAF, Laboratório de Química da Biodiversidade, Avenida Comandante Guaranys, 447, Jacarepaguá, CEP 22775-903, Rio de Janeiro, RJ, Brasil.
have a degree in Pharmacy from the Federal University of Rio de Janeiro (1991), specialization in Industrial Pharmacy from the Federal University of Rio de Janeiro (1992) and a master's degree in Organic Chemistry from the Federal University of Rio de Janeiro (1998). I have extensive experience in the Analytical Organic Chemistry area, with an emphasis on Chemical Metrology, working from project management as well as the development and validation of analytical methods in the areas of food, pharmacy, phytochemistry, phytotherapy, environment, chemistry and oil, employing the techniques of liquid and gas chromatography, mass spectrometry, UV / VIS spectrophotometry, infrared spectrophotometry (FT-IR) and physical-chemical analysis. People, financial, investment, budget and quality management (BPL, NBR ISO 17025, Auditor). Technical consultancy (implementation of new methods in laboratories, technical support) and second part auditing of NBR ISO 17025. Experience in meeting Brazilian (ANVISA, MAPA, INMETRO, Pharmacopéia) norms (ICH, AOAC, USP) ) for the development of analytical methodologies, stability studies, regulatory concentration limits, among others.
Wagner Wollinger
3. Instituto Nacional de Metrologia - INMETRO, Qualidade e Tecnologia, Divisão de Metrologia Química - DIMCI. Avenida Nossa Senhora das Graças, 50, Xerém, CEP 25250-020, Duque de Caxias, RJ, Brasil.
Graduated in Industrial Pharmacy from the Federal University of Paraná (2002), master's degree in Analytical Chemistry from the Federal University of Rio de Janeiro (2008) and doctorate in Chemistry from the Federal University of Rio de Janeiro (2018). Researcher in the area of Chemical Metrology at Inmetro. Experience in the area of Analytical Chemistry, with emphasis on nuclear magnetic resonance and chromatographic analysis of organic compounds. It works with the production of certified reference materials for chemical substances, product testing with a focus on conformity assessment and research of analytical methods.
Claudio Cerqueira Lopes
4. Universidade Federal do Rio de Janeiro, Instituto de Química. Av. Athos da Silveira Ramos 149, CT, Bloco A, sala 508, Cidade Universitária, CEP 21941-909, Rio de Janeiro, RJ, Brasil.
Cláudio Cerqueira Lopes, Federal University of Rio de Janeiro-Institute of Chemistry, Coordinator of the Laboratory for Synthesis and Analysis of Strategic Products (LASAPE). Graduated in Pharmacy (1977) and Industrial Pharmacy (1978), all from the Faculty of Pharmacy - UFRJ, master's degree - IPPN, (1983) and doctorate - Institute of Chemistry (1989), all in Chemistry of Natural Products and Chemistry from UFRJ, post-doctorate in Organic Synthesis at the Department of Chemistry, University of California Berkeley- USA (1989-1991). He is currently Full Professor at UFRJ, starting his teaching career as an assistant professor in 1979. He has experience in the area of Forensic, Organic and Analytical Chemistry, acting mainly on the following themes: synthesis of natural products, products with applications in the forensic area, such as for example : invisible ink, chemical markers for firearm ammunition resulting in bills in the Federal Senate, Chamber of Deputies and Assembly of Deputies of RJ, development of a semen detection kit in rape crime scenes, preparation of strategic products with potential economic exploitation, such as: amino-sugars (MIGLUSTAT), Luminol for detection of occult blood in homicide scenes and to combat hospital contamination, PAF analogs, LYSO-PAF prepared from soy lecithins, biocides with potential application to combat the process of marine bio-encrustation and in the treatment of ballast waters of vessels, AltissimacumarinaD and other subs with relevant biological activities. Analytical development in the area of forensic chemistry, preparation and formulation of the reagent Luminol - UFRJ used by the Civil Police of the State of Rio de Janeiro and the Forensic Expertise of the State of Ceará - Secretariat of Public Security and Social Defense. Has 20 patents in Brazil and USA, deposited, obtained the grant of four, being responsible for generating financial resources to UFRJ, through FUJB-UFRJ, obtained 79 scientific, technological publications and 461 citations, Web of Science ResearchID, P-1252 -2018; Scholarship in Productivity in Technological Development and Innovative Extension, level II of CNPq, index h = 11.0. Scientist of Nosso Estado- FAPERJ (2018), Medal Maria da Penha M. Jacobina, IQ- UFRJ (2017), Titular Member of the Brazilian Academy of Military Medicine (2014), Petrobras Technology Award in the 6th edition (2013), Amigo Award the Brazilian Navy (2011), Amigo da Caserna, Eastern Military Command 1st Army Division (2010), Citation of the Miglustat synthesis in the 14th Edition of The Index Merck, US (2009); ABIQUIM Technology Award (2008); Tribute in favor of Forensic Sciences-CREMERJ (2008); citation of the importance of LUMINOL in criminal investigation in the book Química 2, Ricardo Feltre for high school (2008), citation of work due to 100,000 online accesses in the Beilstein Journal of Organic Chemistry Commemorative Book (2007), Police Friend Award Civil of the State of Rio de Janeiro - SSP / RJ (2005).
Rosangela Sabbatini Capella Lopes
4. Universidade Federal do Rio de Janeiro, Instituto de Química. Av. Athos da Silveira Ramos 149, CT, Bloco A, sala 508, Cidade Universitária, CEP 21941-909, Rio de Janeiro, RJ, Brasil.
Graduated in Pharmacy from the Federal University of Rio de Janeiro (1977), Master in Chemistry of Natural Products from the Federal University of Rio de Janeiro (1983), PhD in Organic Chemistry from the Federal University of Rio de Janeiro (1989) and post-doctorate by UC Berkeley- USA (1989-1991). She is currently Full Professor at the Federal University of Rio de Janeiro, Institute of Chemistry - Department of Analytical Chemistry. Coordinator of the Strategic Products Synthesis and Analysis Laboratory (LASAPE), IQ-UFRJ. He has experience in the area of Chemistry, with an emphasis on Organic Synthesis and Analytical Development, acting mainly on the following themes: synthesis of natural biocides from residues from the edible oil and biodiesel industry, soy lecithins and glycerin, with potential use in the treatment ballast water from vessels, aiming to prevent the spread of invasive species in the Brazilian marine environment and incorporated into antifouling paints with potential use to prevent the natural process of bio-encrustation on metallic surfaces. In the area of analytical development, he worked on the development of a luminol kit with two sprinklers and another associated with a portable luminometer, to detect traces of occult blood in homicide sites. The reagent Luminol-UFRJ is being used in the criminal investigation activities of the Homicide Division (DH), Carlos Eboli Criminal Institute (ICCE) of the Civil Police of the State of Rio de Janeiro and by the Forensic Expert of Ceará - Technical-Scientific Department of Judicial Police of the State of Ceará. He is currently developing the synthesis of wedelolactone, a cumestane with potent anti-snakebite and anti-tumor activity. Its 42 published works and 20 patents deposited, 4 of which were granted at the National Institute of Industrial Property (INPI) and the United States Patent Office (USPTO), this set of scientific and technological production obtained a total of 393 citations, providing an index 11.0 h, according to the Web of Science. Researcher in Technological Development and Innovative Extension level II of CNPq.
Selma Ribeiro Paiva
2. Universidade Federal Fluminense, Faculdade de Farmácia, Pós-Graduação e Ciências Aplicadas a Produtos para Saúde, Rua Mário Viana, 523, Santa Rosa, Cep: 24.241-000, Niterói, RJ, Brasil. 5. Universidade Federal Fluminense - UFF, Centro de Estudos Gerais, Instituto de Biologia, Outeiro de São João Batista, s/n, Centro, CEP 24020-150, Niteroi, RJ, Brasil.
Graduated in Biological Sciences from the Federal University of Rio de Janeiro (1996), master's degree in Biological Sciences (Botany) from the Federal University of Rio de Janeiro (1999) and doctorate in Plant Biotechnology from the Federal University of Rio de Janeiro (2003). She is currently an associate professor at the Department of General Biology, Institute of Biology, Fluminense Federal University and vice-coordinator of the Postgraduate Program in Sciences Applied to Health Products (UFF). Has experience in Botany, acting on the following subjects: phytochemistry, pharmacobotany and chemical ecology.
Glaucia Barbosa Candido Alves Slana
National Institute of Industrial Property - INPI, Rua São Bento, 1, Centro, CEP 20090-010, Rio de Janeiro, RJ, Brazil.
Graduated in Pharmacy from the Federal University of Rio de Janeiro (1995), Master in Chemistry from the Federal University of Rio de Janeiro (1998) sandwich doctorate in Medicinal Chemistry from the Federal University of Rio de Janeiro (2003) and Queen`s University (Canada ) and Post-Doc in Inorganic Chemistry by the Pontifical Catholic University. He currently teaches and conducts research at the Federal University of Rio de Janeiro and at the Institute of Technology in Pharmaceuticals - Farmanguinhos / FIOCRUZ. He has experience in the area of Chemistry with an emphasis on Analytical Chemistry in the development and validation of analytical methods applied to the development and analysis of drugs and correlates and synthesis of pharmaceuticals of natural origin via lithium reactions. The aggregate experiences contributed to his experience in Pharmacometry directed to the development of medicines by the development of in-vitro methods for determining their efficacy and safety. Accumulated knowledge in Pharmaceutical Equivalence as coordinator and technician responsible for EQFAR 40, in pharmaceutical ethics, quality assurance and quality control. In the area of management, he worked in the implementation and elaboration of the course of technologist in drugs at UEZO, and structuring of laboratories and courses in Pharmaceutical Analysis and Quality Control at Pharmacy UFRJ-Macaé. He is currently working on the development and evaluation of drugs for tuberculosis and antiretrovirals, assessment of bioavailability and the influence of biopharmaceutical parameters, as well as the development of phytomedicines. In addition to working as a researcher in industrial property. He has guidance from CI students, masters and doctorates in the areas in which he works and supervision in Postdoctoral students.
Considering the need to have a fast, cheap and effective method for quality control routine, thin layer chromatography/densitometry has been a chosen method to comply those requests. However, this technique has its limitations when used to determination assays for substances in complex matrices, such as vegetal extracts. Considering this chromatographic technique restriction during the drug development assay, a new procedure was developed to overcome the difficulty in obtaining the validation parameters within the international acceptance criteria, mainly selectivity and repeatability parameters. The developed method was applied in the betulinic acid (BA) quantification from extracts of natural products. All separations were obtained on plates Si60 F254 with n-hexane:ethyl acetate:acetic acid (7:3:0.3) at 560nm, after derivatization with vanillin-sulphuric reagent. The system gave compact spots for BA (Rf. 0.5) and exhibited linearity (R2= 0.999). The methodology was compared with a validated gas chromatography methodology and no statistical difference was observed.
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Andrea Bezerra da Nóbrega
1. Fundação Oswaldo Cruz – Fiocruz, Instituto de Tecnologia em Fármacos - Farmanguinhos, Plataforma Agroecológica de Fitomedicamentos - PAF, Laboratório de Química da Biodiversidade, Avenida Comandante Guaranys, 447, Jacarepaguá, CEP 22775-903, Rio de Janeiro, RJ, Brasil. 2. 2Universidade Federal Fluminense, Faculdade de Farmácia, Pós-Graduação e Ciências Aplicadas a Produtos para Saúde, Rua Mário Viana, 523, Santa Rosa, Cep: 24.241-000, Niterói, RJ, Brasil.
Bruno da Motta Lessa
1. Fundação Oswaldo Cruz – Fiocruz, Instituto de Tecnologia em Fármacos - Farmanguinhos, Plataforma Agroecológica de Fitomedicamentos - PAF, Laboratório de Química da Biodiversidade, Avenida Comandante Guaranys, 447, Jacarepaguá, CEP 22775-903, Rio de Janeiro, RJ, Brasil
Carlos Henrique Brasil Bizarri
1Fundação Oswaldo Cruz – Fiocruz, Instituto de Tecnologia em Fármacos - Farmanguinhos, Plataforma Agroecológica de Fitomedicamentos - PAF, Laboratório de Química da Biodiversidade, Avenida Comandante Guaranys, 447, Jacarepaguá, CEP 22775-903, Rio de Janeiro, RJ, Brasil.
Wagner Wollinger
3. Instituto Nacional de Metrologia - INMETRO, Qualidade e Tecnologia, Divisão de Metrologia Química - DIMCI. Avenida Nossa Senhora das Graças, 50, Xerém, CEP 25250-020, Duque de Caxias, RJ, Brasil.
Claudio Cerqueira Lopes
4. Universidade Federal do Rio de Janeiro, Instituto de Química. Av. Athos da Silveira Ramos 149, CT, Bloco A, sala 508, Cidade Universitária, CEP 21941-909, Rio de Janeiro, RJ, Brasil.
Rosangela Sabbatini Capella Lopes
4. Universidade Federal do Rio de Janeiro, Instituto de Química. Av. Athos da Silveira Ramos 149, CT, Bloco A, sala 508, Cidade Universitária, CEP 21941-909, Rio de Janeiro, RJ, Brasil.
Selma Ribeiro Paiva
2. Universidade Federal Fluminense, Faculdade de Farmácia, Pós-Graduação e Ciências Aplicadas a Produtos para Saúde, Rua Mário Viana, 523, Santa Rosa, Cep: 24.241-000, Niterói, RJ, Brasil. 5. Universidade Federal Fluminense - UFF, Centro de Estudos Gerais, Instituto de Biologia, Outeiro de São João Batista, s/n, Centro, CEP 24020-150, Niteroi, RJ, Brasil.
Glaucia Barbosa Candido Alves Slana
National Institute of Industrial Property - INPI, Rua São Bento, 1, Centro, CEP 20090-010, Rio de Janeiro, RJ, Brazil.
