Antioxidant and antimicrobial properties of ethanolic extract of Libidibia ferrea pods

Nascimento, P;
Silva, T;
Gomes, J;
Silva, M;
Souza, S;
Falcão, R;
Silva, T;
Moreira, , K.

Patricia Nascimento

Unidade Acadêmica de Garanhuns / Universidade Federal Rural de Pernambuco

Talita Silva

José Gomes

Mitaliene Silva

Silvana Souza

Rosângela Falcão

Tania Silva

Keila Moreira


Resumo

Libidibia ferrea is a typical plant of northern and north-east Brazil and has well-known medicinal properties that can be found in ethanolic extracts such as antipyretic, anti-inflammatory and anti-rheumatic compounds. This study seeks to evaluate the potential antioxidant and antimicrobial activity of ethanol extracts from Libidibia ferrea pods. The pods were used for the preparation of ethanolic extracts of L. ferrea which is used to determine biological activity, and measure their total phenolic content. For quantification of antioxidant methods of DPPH, ABTS and β-carotene were employed and the method of minimum inhibitory concentration (MIC) to measure the antimicrobial activity. The ethanolic extract of L. ferrea showed excellent antioxidant activity, as well as other similar substances commonly used for this purpose such as citric acid and trolox. In addition, it had antimicrobial activity against Gram-negative and Gram-positive bacteria.

Referências

  1. ARAÚJO, T.A.S.; ALENCAR, N.L.; AMORIM, E.L.C.; ALBUQUERQUE, U.P. 2008 - A new approach to study medicinal plants with tannins and flavonoids contents from the local knowledge. Journal of Ethnopharmacology, v.120, p.72–80.
  2. BAMONIRI, A.; EBRAHIMABADI, A.H.; MAZOOCHI, A.; BEHPOUR, M.; KASHI, F.J.; BOUDET, A.M. 2007 - Evolution and current status of research in phenolic compounds. Phytochemistry, v.68, p.22-24.
  3. CARVALHO, J.C.T.; TEIXEIRA, J.R.M.; PERGENTINO, J.C.S.; BASTOS, J.K.; SANTOS FILHO, D.; SARTI, J. 1996 - Preliminary studies of analgesic and anti-inflammatory properties of Caesalpinia ferrea crude extract. Journal of Ethnopharmacology, v.53, p.175-178.
  4. CAVALHEIRO, M.G.; FARIAS, D.F.; FERNANDES, G.S.; NUNES, E.P.; CAVALCANTI, F.S.; VASCONCELOS, I.M.; MELO, V.M.M.; CARVALHO, A.F.U. 2009 - Biological and enzymatic activities of aqueous extract of seeds from Caesalpinia ferrea Mart., Leguminosae. Brazilian Journal of Pharmacognosy, v.19, n2, p.586-591.
  5. CLINICAL AND LABORATORY STANDARDS INSTITUTE. CLSI. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Wayne, PA: National Committee for Clinical Laboratory Standards; M7-A6. 2003.
  6. COS, P.; VLIETINCK, A.J.; BERGHE, D.V.; MAES, L. 2006 - Anti-infective potential of natural products: How to develop a stronger in vitro ‘proof-of-concept’. Journal of Ethnopharmacology, v.106, p.290-302.
  7. FERREIRA, M.R.A.; SOARES, L.A.L. 2015 - Libidibia ferrea (Mart. Ex Tul.) L.P. Queiroz: A review of the biological activities and phytochemical composition. Journal of Medicinal Plants Research, v.9, n.2, p.140-150.
  8. FLOEGEL, A.; KIM, D.O.; CHUNG, S.J.; KOO, S.I.; CHUN, O.K. 2011 - Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. Journal of Food Composition and Analysis, v.24, p.1043–1048.
  9. GHASEMNEZHAD, M.; SHERAFATI, M.; PAYVAST, G.A. 2011 - Variation in phenolic compounds, ascorbic acid and antioxidant activity of five coloured bell pepper (Capsicum annum) fruits at two different harvest times. Journal of Functional Foods, v.3, p.44–49.
  10. HAUNG, D.J.; OU, B.X.; PRIOR, R.L. 2005 - The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry, v.53, p.1841-1856.
  11. KAUR, R.; ARORA, S.; SINGH, B. 2008 - Antioxidant activity of the phenol rich fractions of leaves of Chukrasia tabularis A. Juss. Bioresource Technology, v.99, p.7692–7698.
  12. LIMA, S.M.A.; ARAÚJO, L.C.C.; SITÔNIO, M.M.; FREITAS, A.C.C.; MOURA, S.L.; CORREIA, M.T.S.; MALTA, D.J.N.; GONÇALVES-SILVA, T. 2012 - Anti-inflammatory and analgesic potential of Caesalpinia ferrea. Brazilian Journal of Pharmacognosy, v.2, n.1, p.169-175.
  13. MARTYSIAK-ZUROWSKA, D.; WENTA, W. 2012 - A comparison of ABTS and DPPH methods for assessing the total antioxidant capacity of human milk. Acta Scientiarum Polonorum, Technologia Alimentaria, v.11, n.1, p.83-89.
  14. MBOSSO, E.J.T.; NGOUELA, S.; NGUEDIA, J.C.A.; BENG, V.P.; ROHMER, M.; TSAMO, E. 2010 - In vitro antimicrobial activity of extracts and compounds of some selected medicinal plants from Cameroon. Journal of Ethnopharmacology, v.128, p.476-481.
  15. NAKAMURA, E.S.; KUROSAKI, F.; ARISAWA, M.; MUKAINAKA, T.; OKUDA, M.; TOKUDA, H.; NISHINO, H.; PASTORE, J.F. 2002 - Cancer chemo preventive effects of constituents of Caesalpinia ferrea and related compounds. Cancer Letters, v.177, p.119–124.
  16. OLIVEIRA, L.S.; SANTANA, A.L.B.D.; MARANHÃO, C.A.; MIRANDA, R.C.M.; LIMA, V.L.A.G.; SILVA, S.I.S.; NASCIMENTO, M.S.; BIEBER, L. 2010 - Natural resistance of five woods to Phanerochaete chrysosporium degradation. International Biodeterioration & Biodegradation, v.64, p.711-715.
  17. PEREIRA, L.P.; SILVA, R.O.; BRINGEL, P.H.S.F.; SILVA, K.E.S.; ASSREUY, A.M.S.; PEREIRA, M.G. 2012 - Polysaccharide fractions of Caesalpinia ferrea pods: potential anti-inflammatory usage. Journal of Ethnopharmacology, v.139, p.642–648.
  18. PETERS, V.M.; SOUZA, S.O.; CARVALHO, J.C.T.; BORGES, L.V.; GUERRA, M.O. 2008 - Evaluation of reproductive toxicity of aqueous extract of the fruits from Caesalpinia ferrea Mart. in rats. Boletín latinoamericano y del Caribe de plantas medicinales y aromáticas, v.7, n.5, p.268-272.
  19. RE, R.; PELLEGRINI, N.; PROTEGGENTE, A.; PANNALA, A.; YANG, M.; RICE-EVANS, C. 1999 - Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine, v.26, p.11231-1237.
  20. ROGINSKY, V.; LISSI, E.A. 2005 - Review of methods to determinate chain-breaking antioxidant activity in food. Food Chemistry, v.92, p.235-254.
  21. SAMPAIO, F.C.; PEREIRA, M.S.V.; DIAS, C.S.; COSTA, V.C.O.; CONDE, N.C.O.; BUZALAF, M.A.R. 2009 - In vitro antimicrobial activity of Caesalpinia ferrea Martius fruits against oral pathogens. Journal of Ethnopharmacology, v.124, p.289–294
  22. SHARMA, O.P.; BHAT, T.K. 2009 - DPPH antioxidant assay revisited. Food Chemistry, v.113, p.1202–1205.
  23. SILVA, L.C.N.; SILVA-JÚNIOR, C.A.; SOUZA, R.M.; MACEDO, A.J.; SILVA, M.V.; CORREIA, M.T.S. 2011 - Comparative analysis of the antioxidant and DNA protection capacities of Anadenanthera colubrina, Libidibia ferrea and Pityrocarpa moniliformis fruits. Food and Chemical Toxicology, v.49, p.2222–2228.
  24. SIQUEIRA, C.F.Q.; CABRAL, D.L.V.; SOBRINHO, T.J.S.P.; AMORIM, E.L.C.; MELO, J.G.; ARAÚJO, T.A.S. 2012 - Albuquerque UP. Levels of tannins and flavonoids in medicinal plants: evaluating bioprospecting strategies. Evidence-Based Complementary and Alternative Medicine Article ID 434782, doi:10.1155/2012/434782.
  25. SLINKARD, K.; SINGLETON, V.L. 1977 - Total phenol analyses: Automation and comparison with manual methods. American Journal of Enology Viticulture, v.28, p.49–55.
  26. SOUZA, A.B.; SOUZA, L.M.S.; CARVALHO, J.C.T.; MAISTRO, E.L. 2006 - No clastogenic activity of Caesalpinia ferrea Mart. (Leguminosae) extract on bone marrow cells of Wistar rats. Genet Genetics and Molecular Biology, v.29, p.380–383.
  27. SURVESWARAN, S.; CAI, Y.Z.; CORKE, H.; SUN, M. 2007 - Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food Chemistry, v.102, p.938–953.
  28. VASCONCELOS, C.F.B.; MARANHÃO, H.M.L.; BATISTA, T.M.; CARNEIRO, E.M.; FERREIRA, F.; COSTA, J.; SOARES, L.A.L.; SÁ, M.D.C.; SOUZA, T.P.; WANDERLEY, A.G. 2011 - Hypoglycaemic activity and molecular mechanisms of Caesalpinia ferrea Martius bark extract on streptozotocin-induced diabetes in Wistar rats. Journal of Ethnopharmacology, v.137, p.1533–1541.
  29. THE INTERNATIONAL PLANT NAMES INDEX (IPNI), 1 March 2009. Disponível em <http://www.ipni.org/> Acesso 25 ago. 2015.