May Plantago major L. be used as an antimicrobial agent for use in oral application products?

Maria Luiza Nogueira
Leandro Martins Lima
OrcID
Ari Sérgio de Oliveira Lemos
Marcel Arruda Diogo
Thalita de Freitas Souza
João Paulo Santana da Silva
Ademar Alves da Silva Filho
Luciana Moreira Chedier
Rodrigo Luiz Fabri
Priscila de Faria Pinto

    Maria Luiza Nogueira

    Universidade Federal de Juiz de Fora (UFJF), Departamento de Bioquímica, Instituto de Ciências Biológicas - ICB, Campus Universitário, Rua José Lourenço Kelmer, s/n, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brasil.

    Graduanda em farmácia pela Universidade Federal de Juiz de Fora, onde atua como integrante do grupo de pesquisa Estrutura e Função de Proteínas sobre orientação da Dr. Priscila de Faria Pinto. Neste grupo tem trabalhado nas áreas de microbiologia e produtos naturais. Como acadêmica já participou da Empresa Ecofarma Júnior como trainee e consultora de projetos. Também já participou de programas de monitoria das disciplinas Sistemas de Saúde e Saúde Coletiva e Epidemiologia. Além disso, já participou de diversos cursos e eventos científicos. Atualmente, é monitora da disciplina de Farmacotécnica, atua como ligante na Liga Acadêmica de Farmacologia Clínica e Farmacoterapia e é diretora da Liga Acadêmica de Toxicologia.

    Leandro Martins Lima

    Federal University of Juiz de Fora (UFJF), Department of Biochemistry, Institute of Biological Sciences - ICB, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.

    OrcID https://orcid.org/0000-0002-8491-4883

    Adult Health expert, with emphasis on clinical analysis, by the Multi-professional Residency Program in Adult Health of University Hospital of the Federal University of Juiz de Fora - UFJF (since 2019). Expert in Microbiology be the Faculty of Medical and Health Sciences of Juiz de Fora - FCMS/JF (since 2018). Graduated in Pharmaceutical Sciences, with emphasis on Clinical Analysis, from Federal University of Juiz de Fora - UFJF (since 2016). Currently , I am studying for a Master`s degree in Biological Sciences at the Graduate Program in Biology at the Federal University of Juiz de Fora - UFJF.

    Ari Sérgio de Oliveira Lemos

    Federal University of Juiz de Fora (UFJF), Department of Biochemistry, Institute of Biological Sciences - ICB, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.

    He holds a master's degree in biological sciences from the Federal University of Juiz de Fora (2019), a degree in Pharmacy from the Federal University of Juiz de Fora (2016). He conducts research related to the discovery of bioactive molecules of plant origin for the treatment of microbial infections, oxidative stress and inflammatory processes. He has experience in the area of isolation and identification of secondary metabolites from plant species and chromatographic analysis.

    Marcel Arruda Diogo

    Federal University of Juiz de Fora (UFJF), Department of Biochemistry, Institute of Biological Sciences - ICB, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.

    Graduated in Pharmacy from the Federal University of Juiz de Fora and currently a Master's student in the Postgraduate Program in Applied Biochemistry at the Federal University of Viçosa working on the following topics: Biochemistry and Molecular Biology, Study of the Structure and Function of Proteins, Molecular Infectology, Rational Design of Drugs and Pharmaceutical Supplies for Diagnosis of Infectious Diseases and Expression and Purification of Recombinant Proteins.

    Thalita de Freitas Souza

    Federal University of Juiz de Fora (UFJF), Department of Botany, Institute of Biological Sciences, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.

    Has experience in the field of Pharmacy, with an emphasis on Clinical Analysis. He is currently a member of the Laboratory of Bioactive Natural Products at the Institute of Biological Sciences - UFJF.

    João Paulo Santana da Silva

    Federal University of Juiz de Fora (UFJF), Department of Biochemistry, Institute of Biological Sciences - ICB, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.

    Dentistry Student at the Federal University of Juiz de Fora, starting in 2016 and ending in 2021. Dr. Gracieli Prado Elias. He is also a volunteer in the Só-riso Extension Project - Maternal and Child Care at UFJF, under the guidance of Prof. Dr. Cristina Lougon Borges de Mattos and the Clinical Extension Project for adolescents, under the guidance of Prof. Dr. Elton Geraldo de Oliveira Gois. He is secretary of the Academic League for Integral Health of the LGBTTI+ Population at UFJF, he is also a member of the National League of Pediatric Dentistry associated with ABOPed, of the Pediatric Dentistry Academic League of Technology and Sciences, of the Psychosocial Dentistry Academic League and of the Health Nursing Academic League of Woman and the Newborn. He is a monitor of Pediatric Dentistry at the Department of Social and Child Dentistry, at the UFJF School of Dentistry. He was a fellow at the Department of Parasitology, Microbiology and Immunology at UFJF, in the Scientific Initiation Project Analysis of the clinical-epidemiological and behavioral profile of individuals who make regular use of mouthwashes and their perception of the role of microorganisms in oral health, under supervision of Prof.ª and Dr. Ana Carolina Morais Apolônio. In addition, he has technical training in Metallurgy at the institution IF Sudeste MG, campus Juiz de Fora, where he also attended high school, from 2013 to 2015. http://orcid.org/0000-0003-1859-3054

    Ademar Alves da Silva Filho

    Federal University of Juiz de Fora (UFJF), Department of Pharmaceutical Sciences, Faculty of Pharmacy, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.

    Graduated in Pharmacy from the Federal University of Juiz de Fora (1998) and Masters (2001) and Doctorate (2006) in Pharmaceutical Sciences (Drugs and Medicines) from the Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (FCFRP - USP) . During his Ph.D., he held a Sandwich Internship Abroad (PDEE-Capes) at the National Center for Natural Products Research (NCNPR), Research Institute of Pharmaceutical Sciences, University of Mississippi (Oxford, MS, USA). He is currently Associate Professor II at the Federal University of Juiz de Fora. He has an H index = 29 (ISI), H = 31 (Scopus), an i10 google index = 62 (since 2016) and in recent years he has published several full articles in specialized international journals in the area of ​​chemistry and biological activity of natural products. He participated in the development of technological and innovation products, which resulted in the filed patents. He received awards and honors and coordinated the Young Researcher Program project at an emerging research center funded by FAPESP, as well as participating as a collaborator in several research support projects. In his professional activities, he interacted with several collaborators in co-authorship of scientific papers. He is a member of the editorial board of specialized international journals and a reviewer of national and international periodicals. He is currently a member of the Graduate Programs in Pharmaceutical Sciences (CAPES 4) and Chemistry (CAPES 5) at UFJF. It also collaborates and coordinates research support projects FAPEMIG, CNPq and institutional subprojects CTINFRA/FINEP/UFJF, in addition to guiding and co-supervising PhD, Masters and scientific initiation students and supervising postdoctoral students in the areas of Chemistry and Activity Natural Products, acting on the following subjects: schistosomicidal, antiparasitic, immunomodulatory, anti-inflammatory and bioprospecting and chemical activities of natural compounds.

    Luciana Moreira Chedier

    Federal University of Juiz de Fora (UFJF), Department of Botany, Institute of Biological Sciences, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.

    Graduated in Biological Sciences from the Federal University of Rio de Janeiro (1992), Masters in Biological Sciences (Botany) from the National Museum, Federal University of Rio de Janeiro (1995) and Ph.D. in Biological Sciences (Botany) from the National Museum, Federal University of Rio de Janeiro (2002). He is currently Associate Professor II at the Federal University of Juiz de Fora. She is the coordinator of PROFBIO / UFJF / JF-Professional Master's Degree in Biology Teaching in a National Network and a professor on its permanent staff. She has experience in teaching, research and technological development in the field of Botany, with an emphasis on Applied Botany, acting on the following topics: Chemistry of Natural Products, Biological Activities and Ethnopharmacology.

    Rodrigo Luiz Fabri

    Federal University of Juiz de Fora (UFJF), Department of Biochemistry, Institute of Biological Sciences - ICB, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.

    Graduated in Pharmacy and Biochemistry from the Federal University of Juiz de Fora (UFJF) (2006) and Masters (2006-2008)/Doctorate (2011-2013) in Biological Sciences (concentration area: Genetics and Biotechnology) from the same institution. He is currently Head and Adjunct Professor of the Department of Biochemistry at UFJF, teaching Basic Biochemistry for the Veterinary Medicine course. He guides undergraduate students in the modalities of monitoring, professional training, extension and scientific initiation, and stricto sensu graduate students. He is part of the faculty of the Graduate Program in Pharmaceutical Sciences (PPGCF) and the Professional Master's Degree in Biology Teaching (PROFBIO) at UFJF. He has experience in the area of ​​Natural Products, with an emphasis on Chemistry and Pharmacology of Bioactive Natural Products, acting mainly in: (i) preparation, extraction and fractionation of plant extracts, (ii) isolation and elucidation of bioactive substances, (iii) standardization of extracts by HPLC/DAD, (iv) chemical characterization of extracts by CG/MS and UPLC-QTOF-MS and (v) evaluation of biological activities (cytotoxic, anti-inflammatory, antinociceptive, antimicrobial and antioxidant). In addition, he also works in the area of ​​Biochemistry, on the following topics: (i) assay of antioxidant enzymes, (ii) analysis of biochemical and hematological parameters in in vivo tests, and (iii) lipid characterization of biological samples.

    Priscila de Faria Pinto

    Federal University of Juiz de Fora (UFJF), Department of Biochemistry, Institute of Biological Sciences - ICB, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.

    PhD in Science (Infectious and Parasitic Diseases) from CPqRR/FIOCRUZ/MG, Masters in Cellular and Molecular Biology from IOC/FIOCRUZ/RJ and graduation in Pharmacy and Biochemistry from the Federal University of Juiz de Fora. She is currently an Adjunct Professor at the Department of Biochemistry at UFJF in the city of Juiz de Fora. Researcher and postgraduate advisor for the Faculty of Pharmacy program (Pharmaceutical Sciences) and the Institute of Biological Sciences (Infectious-Parasitic Disease). She has experience in the field of Biochemistry, with an emphasis on Biochemistry of macromolecules, acting on the following topics: purification of proteins from plant origin; study of the family of ATP diphosphohydrolases of parasites and analysis of molecular markers for infectious parasitic diseases and search for molecules of synthetic and plant origin with schistosomicidal action.

Keywords

Plantago major
Medicinal plants
Phytotherapy
Antimicrobial agents
Chromatography thin layer

Abstract

Plantago major L. has been widely used in the treatment of wounds and coughs due to its anti-inflammatory, healing and antimicrobial potential, according to popular indications. Aim at the use of this plant in the formulation of oral products, the objective of this work was to evaluate the antimicrobial activity of P. major extracts, in vitro. Ethanolic and hydroalcoholic extracts were prepared with dry aerial parts of the plant, which were submitted to phytochemical screening and analyzed by thin layer chromatography (TLC). The antimicrobial activity of the extracts and of a commercial tincture was performed by agar diffusion. Phytochemical screening showed presence of phenolic substances, cumarines and triterpenes, in addition to a low concentration of phenols and flavonoids in the extracts. The bands observed in TLC did not demonstrate 5-O-caffeoylquinic acid, a chemical marker, however the number of substances classes found and the presence of a probable 5-O-caffoylquinic acid derivative are consistent with P. major specimens. In agar diffusion test, the extracts and tincture were not able to inhibit the bacterial growth, however its anti-inflammatory activity should not be discarded due to the substances found and its synergic application with others antimicrobial agents to developing of oral use products.

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Author(s)

  • Maria Luiza Nogueira
    Universidade Federal de Juiz de Fora (UFJF), Departamento de Bioquímica, Instituto de Ciências Biológicas - ICB, Campus Universitário, Rua José Lourenço Kelmer, s/n, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brasil.
  • Leandro Martins Lima
    Federal University of Juiz de Fora (UFJF), Department of Biochemistry, Institute of Biological Sciences - ICB, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.
    https://orcid.org/0000-0002-8491-4883
  • Ari Sérgio de Oliveira Lemos
    Federal University of Juiz de Fora (UFJF), Department of Biochemistry, Institute of Biological Sciences - ICB, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.
  • Marcel Arruda Diogo
    Federal University of Juiz de Fora (UFJF), Department of Biochemistry, Institute of Biological Sciences - ICB, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.
  • Thalita de Freitas Souza
    Federal University of Juiz de Fora (UFJF), Department of Botany, Institute of Biological Sciences, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.
  • João Paulo Santana da Silva
    Federal University of Juiz de Fora (UFJF), Department of Biochemistry, Institute of Biological Sciences - ICB, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.
  • Ademar Alves da Silva Filho
    Federal University of Juiz de Fora (UFJF), Department of Pharmaceutical Sciences, Faculty of Pharmacy, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.
  • Luciana Moreira Chedier
    Federal University of Juiz de Fora (UFJF), Department of Botany, Institute of Biological Sciences, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.
  • Rodrigo Luiz Fabri
    Federal University of Juiz de Fora (UFJF), Department of Biochemistry, Institute of Biological Sciences - ICB, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.
  • Priscila de Faria Pinto
    Federal University of Juiz de Fora (UFJF), Department of Biochemistry, Institute of Biological Sciences - ICB, University Campus, Rua José Lourenço Kelmer, no number, São Pedro, CEP 36036-900, Juiz de Fora, MG, Brazil.

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May Plantago major L. be used as an antimicrobial agent for use in oral application products?. Rev Fitos [Internet]. 2021 Sep. 30 [cited 2025 Mar. 11];15(3):354-65. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1005
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