Caracterização dos compostos fenólicos, avaliação da toxicidade e análise da qualidade de amostras de chá de Miconia albicans

Bruna Hamann; Elisa Mara dos Santos Winter; Diogo Alexandre Silbert; Gustavo Amadeu Micke; Luciano Vitali; Adrielli Tenfen; Lara Almida Zimmermann

doi:10.32712/2446-4775.2020.1055

Characterization of phenolic compounds, toxicity assessment, and quality analysis of tea samples from Miconia albicans

Vol. 14 No. 4 (2020)
450-461
19/07/2020
18/12/2020
https://doi.org/10.32712/2446-4775.2020.1055

Bruna Hamann
Elisa Mara dos Santos Winter
Diogo Alexandre Silbert
Gustavo Amadeu Micke
Luciano Vitali
Adrielli Tenfen
Lara Almida Zimmermann

Keywords

Phytochemical analysis.

Tea

Artemia salina

Chromatography

Abstract

The use of medicinal plants as a therapeutic alternative is growing in society. The species Miconia albicans, popularly known as old cinnamon, is being consumed in several regions of the country, as it has anti-inflammatory action. This work evaluated the volatile constituents and the quality of five samples of M. albicans teas, acquired in commercial environments in the city of Jaraguá do Sul. The information on their labels were compared and the presence of foreign matter, microorganisms, moisture content, and total ash were determined. The characterization of phenolic compounds in the samples was performed using HPLC-LC-ESI-MS / MS. Toxicity was assessed with Artemia salina larvae. Some samples showed irregularities regarding the specifications of packaging labels. The results of quality control of the vegetable raw material have shown to be in accordance with the specifications described in the Brazilian Pharmacopeia. The phenolic compounds analysis of five samples, as well as the phytochemical profile consistent with the species under study. The analyzed samples are potentially cytotoxic, which makes the species promising for future studies in the cancer’s treatment.

References

Xia E-H, Zhang H-B, Sheng J et al. The Tea Tree Genome Provides Insights into Tea Flavor and Independent Evolution of Caffeine Biosynthesis. Mol Plant. 2017; 10(6): 866-877. [CrossRef].
Gilbert N. The science of tea’s mood-altering magic. Nature 2019; 566: S8-S9. [CrossRef] [Link].
Salvador MJ, Lopes GN, Nascimento Filho VF et al. Quality control of commercial tea by x-ray fluorescence. X-Ray Spectrom. 2002; 31(2): 141-144. [CrossRef] [Link].
Madeira F, Park YM, Lee J et al. The EMBL-EBI search and sequence analysis tools APIs in 2019. Nucleic Acids Res. 2019; 47(W1): W636-W641. [CrossRef] [Link].
Sortibrán AC, Téllez MGO, Ocotero VM et al. Chronic Toxicity, Genotoxic Assay, and Phytochemical Analysis of Four Traditional Medicinal Plants. J Med Food. 2011; 14(9): 1018-1022. [CrossRef] [Link].
Martins AB, Semir J, Goldenberg R et al. O gênero Miconia Ruiz & Pav. (Melastomataceae) no Estado de São Paulo. Acta Bot Bras. 1996; 10: 267–316. [CrossRef] [Link].
Camargo MGG, Schaefer HM, Habermann G et al. Bicolored display of Miconia albicans fruits: Evaluating visual and physiological functions of fruit colors. Am J Bot. 2015; 102(9): 1453-1461. [CrossRef] [Link].
Alves TMA, Silva AF, Brandão M et al. Biological screening of Brazilian medicinal plants. Mem Inst Oswaldo Cruz. 2000; 95(3): 367-373. ISSN 1678-8060. [CrossRef].
Pieroni LG, Rezende FM, Ximenes VF et al. Antioxidant activity and total phenols from the methanolic extract of Miconia albicans (Sw.) Triana Leaves. Molecules. 2011; 16(11): 9439-9450. [CrossRef] [Link].
Celotto AC, Nazario DZ, Spessoto MA et al. Evaluation of the in vitro antimicrobial activity of crude extracts of three Miconia species. Braz J Microbiol. 2003; 34(4): 339-340. ISSN 1678-4405. [CrossRef].
Vasconcelos MAL, Royo VA, Ferreira DS et al. In vivo analgesic and anti-Inflammatory activities of ursolic acid and oleanoic acid from Miconia albicans (Melastomataceae). Z Naturforsch C J Biosci. 2006; 61(7-8): 477-482. [CrossRef] [PubMed].
Lima RCL, Kongstad TK, Kato L et al. High-Resolution PTP1B Inhibition Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of PTP1B Inhibitors from Miconia albicans. Molecules. 2018; 23(7): 1755. [CrossRef] [PubMed].
Serpeloni JM, Bisarro dos Reis M, Rodrigues J et al. In vivo assessment of DNA damage and protective effects of extracts from Miconia species using the comet assay and micronucleus test. Mutagenesis. 2008; 23(6): 501-507. [CrossRef] [Link].
Mara Serpeloni J, Mazzaron Barcelos GR, Prates Mori M et al. Cytotoxic and mutagenic evaluation of extracts from plant species of the Miconia genus and their influence on doxorubicin-induced mutagenicity: An in vitro analysis. Exp Toxicol Pathol. 2011; 63(5): 499-504. [CrossRef] [Link].
Zhu M-Z, Wen B, Wu H et al. The Quality Control of Tea by Near-Infrared Reflectance (NIR) Spectroscopy and Chemometrics. J Spectrosc. 2019; 2019: 1-11. [CrossRef] [Link].
Brasil. Ministério da Saúde. Agência Nacional de Vigilância Sanitária. ANVISA. Farmacopeia Brasileira. 5a edição. Brasília, 2010. Disponível em: [Link].
Brasil. Ministério da Saúde. Agência Nacional de Vigilância Sanitária. ANVISA. RDC Nº 360, de 23 de dezembro de 2003. Brasília, 2003. Disponível em: [Link].
Brasil. Ministério da Saúde. Secretaria de Vigilância em Saúde. Portaria no 519, de 26 de junho de 1998. Brasilia, 1998. Disponível em: [Link].
Brasil. Ministério da Agricultura, Pecuária e Abastecimento. RDC No 259, de 20 de setembro de 2002. Brasília, 2002. Disponível em: [Link].
Brasil. Ministério da Saúde. Agência Nacional de Vigilância Sanitária. ANVISA. RDC No 277, de 22 de setembro de 2005. Brasília, 2005. Disponível em: [Link].
Wagner H, Bladt S. Plant Drug Analysis. Second Edi. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. ISBN: 978-3-642-00574-9. [CrossRef] [Link].
Siebert DA, Mello F, Alberton MD et al. Determination of acetylcholinesterase and Î±-glucosidase inhibition by electrophoretically-mediated microanalysis and phenolic profile by HPLC-ESI-MS/MS of fruit juices from Brazilian Myrtaceae Plinia cauliflora (Mart.) Kausel and Eugenia uniflora L. Nat Prod Res. 2019; 34(18):2683-2688. [CrossRef].
Meyer B, Ferrigni N, Putnam J et al. Brine Shrimp: a convenient general bioassay for active plant constituents. Pl Med. 1982; 45(5): 31-34. [CrossRef] [PubMed].
World Health Organization - WHO. Quality control methods for herbal materials. Press. Geneva, 2011.
Brasil. Agência Nacional de Vigilância Sanitária – ANVISA. Farmacopeia Brasileira. In: Atheneu (Hrsg.). Farmacopeia Brasileira. São Paulo, 1988. ISBN 13: 9788574540573. [Link].
Bernardes LSC, Lang KL, Petrovick PR et al. Produtos Naturais e o desenvolvimento de Fármacos. In: Simoes Cmo, Schenkel Ep, Mentz LA (Hrsg.). Farmacognosia do produto natural ao medicamento. Porto Alegre-RS: Artmed/Grupo A, 2017: 502.
Nnane IP, Hutt AJ, Damani LA. Appendix 1. Essential Guides for Isolation/Purification of Drug Metabolites*. In: Encyclopedia of Separation Science. Elsevier, 2000: 4539-4547. [Link].
Macari PAT, Emerenciano VP, Ferreira ZMGS. Identificação dos triterpenos de Miconia albicans Triana através de análise por microcomputador. Quim Nova. 1990; 13(4): 260–262. [Link].
Oh M-M, Trick HN, Rajashekar CB. Secondary metabolism and antioxidants are involved in environmental adaptation and stress tolerance in lettuce. J Plant Physiol. 2009; 166(2): 180-191. [CrossRef] [PubMed] [Link].
Sampaio BL, Edrada-Ebel R, Da Costa FB. Effect of the environment on the secondary metabolic profile of Tithonia diversifolia: a model for environmental metabolomics of plants. Sci Rep. 2016; 6: 29265. [CrossRef].
Liu W, Yin D, Li N et al. Influence of environmental factors on the active substance production and antioxidant activity in Potentilla fruticosa L. and Its Quality Assessment. Sci Rep. 2016; 6: 28591. [CrossRef] [PubMed] [Link].
Parra AL, Yhebra RS, Sardiñas IG, Buela LI. Comparative study of the assay of and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts. Phytomedicine. 2001; 8(5): 395-400. Disponível em: [Link].
Silva M, David JP, Silva LCRC et al. Bioactive Oleanane, Lupane and UrsaneTriterpene acid derivatives. Molecules. 2012; 17: 12197-12205. [CrossRef] [PubMed] [Link].

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Author(s)

Bruna Hamann
Santa Catarina Educational Society University - UniSociesc, Pharmacy, Jaraguá Park Shopping Campus, Av. Getúlio Vargas, 268, CEP: 89251-970 - Centro, Jaraguá do Sul - SC, Brazil.
Elisa Mara dos Santos Winter
Santa Catarina Educational Society University - UniSociesc, Pharmacy, Jaraguá Park Shopping Campus, Av. Getúlio Vargas, 268, CEP: 89251-970 - Centro, Jaraguá do Sul - SC, Brazil.
Diogo Alexandre Silbert
Federal University of Santa Catarina - UFSC, Graduate Program in Chemistry. Chemistry Department - CFM. Trindade University Campus. ZIP Code: 88040-900 - Florianópolis - SC
Gustavo Amadeu Micke
Federal University of Santa Catarina - UFSC, Graduate Program in Chemistry. Chemistry Department - CFM. Trindade University Campus. ZIP Code: 88040-900 - Florianópolis - SC
Luciano Vitali
Federal University of Santa Catarina - UFSC, Graduate Program in Chemistry. Chemistry Department - CFM. Trindade University Campus. ZIP Code: 88040-900 - Florianópolis - SC
Adrielli Tenfen
Santa Catarina Educational Society University - UniSociesc, Pharmacy, Jaraguá Park Shopping Campus, Av. Getúlio Vargas, 268, CEP: 89251-970 - Centro, Jaraguá do Sul - SC, Brazil.
Lara Almida Zimmermann
Santa Catarina Educational Society University - UniSociesc, Pharmacy, Jaraguá Park Shopping Campus, Av. Getúlio Vargas, 268, CEP: 89251-970 - Centro, Jaraguá do Sul - SC, Brazil.
https://orcid.org/0000-0002-3369-1480

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How to Cite

Characterization of phenolic compounds, toxicity assessment, and quality analysis of tea samples from Miconia albicans. Rev Fitos [Internet]. 2020 Dec. 18 [cited 2025 Feb. 16];14(4):450-61. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1055

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] Xia E-H, Zhang H-B, Sheng J et al. The Tea Tree Genome Provides Insights into Tea Flavor and Independent Evolution of Caffeine Biosynthesis. Mol Plant. 2017; 10(6): 866-877. [CrossRef].

[2] Gilbert N. The science of tea’s mood-altering magic. Nature 2019; 566: S8-S9. [CrossRef] [Link].

[3] Salvador MJ, Lopes GN, Nascimento Filho VF et al. Quality control of commercial tea by x-ray fluorescence. X-Ray Spectrom. 2002; 31(2): 141-144. [CrossRef] [Link].

[4] Madeira F, Park YM, Lee J et al. The EMBL-EBI search and sequence analysis tools APIs in 2019. Nucleic Acids Res. 2019; 47(W1): W636-W641. [CrossRef] [Link].

[5] Sortibrán AC, Téllez MGO, Ocotero VM et al. Chronic Toxicity, Genotoxic Assay, and Phytochemical Analysis of Four Traditional Medicinal Plants. J Med Food. 2011; 14(9): 1018-1022. [CrossRef] [Link].

[6] Martins AB, Semir J, Goldenberg R et al. O gênero Miconia Ruiz & Pav. (Melastomataceae) no Estado de São Paulo. Acta Bot Bras. 1996; 10: 267–316. [CrossRef] [Link].

[7] Camargo MGG, Schaefer HM, Habermann G et al. Bicolored display of Miconia albicans fruits: Evaluating visual and physiological functions of fruit colors. Am J Bot. 2015; 102(9): 1453-1461. [CrossRef] [Link].

[8] Alves TMA, Silva AF, Brandão M et al. Biological screening of Brazilian medicinal plants. Mem Inst Oswaldo Cruz. 2000; 95(3): 367-373. ISSN 1678-8060. [CrossRef].

[9] Pieroni LG, Rezende FM, Ximenes VF et al. Antioxidant activity and total phenols from the methanolic extract of Miconia albicans (Sw.) Triana Leaves. Molecules. 2011; 16(11): 9439-9450. [CrossRef] [Link].

[10] Celotto AC, Nazario DZ, Spessoto MA et al. Evaluation of the in vitro antimicrobial activity of crude extracts of three Miconia species. Braz J Microbiol. 2003; 34(4): 339-340. ISSN 1678-4405. [CrossRef].

[11] Vasconcelos MAL, Royo VA, Ferreira DS et al. In vivo analgesic and anti-Inflammatory activities of ursolic acid and oleanoic acid from Miconia albicans (Melastomataceae). Z Naturforsch C J Biosci. 2006; 61(7-8): 477-482. [CrossRef] [PubMed].

[12] Lima RCL, Kongstad TK, Kato L et al. High-Resolution PTP1B Inhibition Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of PTP1B Inhibitors from Miconia albicans. Molecules. 2018; 23(7): 1755. [CrossRef] [PubMed].

[13] Serpeloni JM, Bisarro dos Reis M, Rodrigues J et al. In vivo assessment of DNA damage and protective effects of extracts from Miconia species using the comet assay and micronucleus test. Mutagenesis. 2008; 23(6): 501-507. [CrossRef] [Link].

[14] Mara Serpeloni J, Mazzaron Barcelos GR, Prates Mori M et al. Cytotoxic and mutagenic evaluation of extracts from plant species of the Miconia genus and their influence on doxorubicin-induced mutagenicity: An in vitro analysis. Exp Toxicol Pathol. 2011; 63(5): 499-504. [CrossRef] [Link].

[15] Zhu M-Z, Wen B, Wu H et al. The Quality Control of Tea by Near-Infrared Reflectance (NIR) Spectroscopy and Chemometrics. J Spectrosc. 2019; 2019: 1-11. [CrossRef] [Link].

[16] Brasil. Ministério da Saúde. Agência Nacional de Vigilância Sanitária. ANVISA. Farmacopeia Brasileira. 5a edição. Brasília, 2010. Disponível em: [Link].

[17] Brasil. Ministério da Saúde. Agência Nacional de Vigilância Sanitária. ANVISA. RDC Nº 360, de 23 de dezembro de 2003. Brasília, 2003. Disponível em: [Link].

[18] Brasil. Ministério da Saúde. Secretaria de Vigilância em Saúde. Portaria no 519, de 26 de junho de 1998. Brasilia, 1998. Disponível em: [Link].

[19] Brasil. Ministério da Agricultura, Pecuária e Abastecimento. RDC No 259, de 20 de setembro de 2002. Brasília, 2002. Disponível em: [Link].

[20] Brasil. Ministério da Saúde. Agência Nacional de Vigilância Sanitária. ANVISA. RDC No 277, de 22 de setembro de 2005. Brasília, 2005. Disponível em: [Link].

[21] Wagner H, Bladt S. Plant Drug Analysis. Second Edi. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. ISBN: 978-3-642-00574-9. [CrossRef] [Link].

[22] Siebert DA, Mello F, Alberton MD et al. Determination of acetylcholinesterase and Î±-glucosidase inhibition by electrophoretically-mediated microanalysis and phenolic profile by HPLC-ESI-MS/MS of fruit juices from Brazilian Myrtaceae Plinia cauliflora (Mart.) Kausel and Eugenia uniflora L. Nat Prod Res. 2019; 34(18):2683-2688. [CrossRef].

[23] Meyer B, Ferrigni N, Putnam J et al. Brine Shrimp: a convenient general bioassay for active plant constituents. Pl Med. 1982; 45(5): 31-34. [CrossRef] [PubMed].

[24] World Health Organization - WHO. Quality control methods for herbal materials. Press. Geneva, 2011.

[25] Brasil. Agência Nacional de Vigilância Sanitária – ANVISA. Farmacopeia Brasileira. In: Atheneu (Hrsg.). Farmacopeia Brasileira. São Paulo, 1988. ISBN 13: 9788574540573. [Link].

[26] Bernardes LSC, Lang KL, Petrovick PR et al. Produtos Naturais e o desenvolvimento de Fármacos. In: Simoes Cmo, Schenkel Ep, Mentz LA (Hrsg.). Farmacognosia do produto natural ao medicamento. Porto Alegre-RS: Artmed/Grupo A, 2017: 502.

[27] Nnane IP, Hutt AJ, Damani LA. Appendix 1. Essential Guides for Isolation/Purification of Drug Metabolites*. In: Encyclopedia of Separation Science. Elsevier, 2000: 4539-4547. [Link].

[28] Macari PAT, Emerenciano VP, Ferreira ZMGS. Identificação dos triterpenos de Miconia albicans Triana através de análise por microcomputador. Quim Nova. 1990; 13(4): 260–262. [Link].

[29] Oh M-M, Trick HN, Rajashekar CB. Secondary metabolism and antioxidants are involved in environmental adaptation and stress tolerance in lettuce. J Plant Physiol. 2009; 166(2): 180-191. [CrossRef] [PubMed] [Link].

[30] Sampaio BL, Edrada-Ebel R, Da Costa FB. Effect of the environment on the secondary metabolic profile of Tithonia diversifolia: a model for environmental metabolomics of plants. Sci Rep. 2016; 6: 29265. [CrossRef].

[31] Liu W, Yin D, Li N et al. Influence of environmental factors on the active substance production and antioxidant activity in Potentilla fruticosa L. and Its Quality Assessment. Sci Rep. 2016; 6: 28591. [CrossRef] [PubMed] [Link].

[32] Parra AL, Yhebra RS, Sardiñas IG, Buela LI. Comparative study of the assay of and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts. Phytomedicine. 2001; 8(5): 395-400. Disponível em: [Link].

[33] Silva M, David JP, Silva LCRC et al. Bioactive Oleanane, Lupane and UrsaneTriterpene acid derivatives. Molecules. 2012; 17: 12197-12205. [CrossRef] [PubMed] [Link].