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Capacity for biofilm formation by bacterial strains and antibiofilm action of Lafoensia pacari (Lythraceae) extract

    Danilo Pavão e Pavão

    University of São Paulo (USP). Institute of Biomedical Sciences of (ICB), Av. Lineu Prestes, 1374, Butantã, CEP 05508-000, São Paulo, SP, Brazil.

    OrcID https://orcid.org/0000-0001-7626-7308

    Graduated in Biomedicine at Faculdade Estácio de São Luís with special interest in research in the areas of Molecular Biology and Biochemistry. He was an intern at the Laboratory of Genetics and Molecular Biology-LabGeM at the Federal University of Maranhão developing research related to the diagnosis of infectious diseases, especially Hansen's disease and Leishmaniasis. He is currently doing a master's degree in Biological Sciences (Microbiology) at the Institute of Biomedical Sciences of the University of São Paulo (ICB-USP) working with structural protein biology and drug repositioning for leprosy.

    Francyelle Costa Moraes

    Centro Universitário Estácio São Luís. R. Grande, 1455, Centro, CEP 65000-000, São Luís, MA, Brasil.

    Graduated in Nursing from Centro Universitário do Maranhão-UniCEUMA (2010) and Master in Parasitic Biology-UniCEUMA (2013). He has experience in Clinical Microbiology, with emphasis on Bacteriology, acting mainly on the following topics: Hospital Infection, Microorganisms of clinical interest, Antimicrobial resistance, Virulence Factors (biofilm formation, adhesion, hemolysins, exoenzymes, serum resistance). She was coordinator of the Scientific Initiation Program and member of the Academic Research Center at Faculdade Estácio de São Luís, developing research in the areas of Microbiology, Parasitology, Epidemiology and Natural Extracts. She is currently a professor in the medical course at Faculdade ITPAC Santa Inês.

    Brendha Luanny Moreira Ribeiro

    Centro Universitário Estácio São Luís. R. Grande, 1455, Centro, CEP 65000-000, São Luís, MA, Brasil.

    Has high school education-second-grade Integrated Unit Duque de Caxias (2015). Has experience in the area of General Biology.

    Flávio Guilherme Rodrigues Costa

    Centro Universitário Estácio São Luís. R. Grande, 1455, Centro, CEP 65000-000, São Luís, MA, Brasil.

    Graduated in Biomedicine by the University Center Estácio São Luís and Postgraduate in Image and Radiodiagnosis. He participated as a Researcher in the Surveillance of Violence and Accidents Survey-VIVA (2017) by the Municipal Health Secretariat through the Superintendence of Epidemiological and Sanitary Surveillance in partnership with Faculdade Estácio de Sá. He worked as an intern in Biomedicine in Epidemiological Surveillance in the city of São Luís do Maranhão, assisting in the monitoring of emerging tropical diseases in Public Health and in the development of strategic projects to guide the occurrence of syphilis in pregnancy.

    Marcos Bispo Pinheiro Camara

    Federal University of Maranhão. Av. Dos Portugueses, 1966, Vila Bacanga, CEP 65080-805, São Luís, MA, Brazil.

    Bachelor's degree in Chemistry from the Federal University of Maranhão - UFMA (2013). Technical analyst collaborator at the Sousândrade Foundation to Support UFMA Development in the period (2009/2015). He is currently a student in the Chemistry Degree course at the Federal Institute of Maranhão -IFMA (2015). With expertise in Organic Chemistry, mainly in the following lines of research: Medicinal plants, Identification and Isolation of organic molecules and Biotechnology.

Keywords

Abstract

In recent years, numerous multidrug-resistant microorganisms have emerged. A special mechanism that confers resistance to antibiotics and the immune system is the ability to form biofilm. This structure is a cluster of bacteria surrounded by a polymeric matrix that protects them. The present study sought to quantify and classify the production of biofilm by microorganisms frequently found in Health Care Related Infections (HAIs) and strains isolated from an Intensive Care Unit (ICU), in addition to testing the antibiofilm activity of the leaf extract of the Lafoensia pacari. The biomass quantification method with violet crystal and reading at 570nm was used. Higher production of biofilm was obtained by bacteria from the group of gram-negatives such as Pseudomonas aeruginosa isolated from a mattress and among the gram-positive E. faecalis stands out (ATCC 19429). The plant extract had antibiofilm action in 50% of the microorganisms tested, including among the strongest biofilm-forming agents.

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Author(s)

  • Danilo Pavão e Pavão
    University of São Paulo (USP). Institute of Biomedical Sciences of (ICB), Av. Lineu Prestes, 1374, Butantã, CEP 05508-000, São Paulo, SP, Brazil.
    https://orcid.org/0000-0001-7626-7308
  • Francyelle Costa Moraes
    Centro Universitário Estácio São Luís. R. Grande, 1455, Centro, CEP 65000-000, São Luís, MA, Brasil.
  • Brendha Luanny Moreira Ribeiro
    Centro Universitário Estácio São Luís. R. Grande, 1455, Centro, CEP 65000-000, São Luís, MA, Brasil.
  • Flávio Guilherme Rodrigues Costa
    Centro Universitário Estácio São Luís. R. Grande, 1455, Centro, CEP 65000-000, São Luís, MA, Brasil.
  • Marcos Bispo Pinheiro Camara
    Federal University of Maranhão. Av. Dos Portugueses, 1966, Vila Bacanga, CEP 65080-805, São Luís, MA, Brazil.

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Capacity for biofilm formation by bacterial strains and antibiofilm action of Lafoensia pacari (Lythraceae) extract. Rev Fitos [Internet]. 2021 Jun. 30 [cited 2025 Apr. 17];15(2):153-65. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1073
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