Identification of larvicidal activity of medicinal plants against Aedes aegypti (Diptera: Culicidae)

Ellen Caroline Nobre Santos
OrcID
Taynara Lopes de Araújo
OrcID
Andson Amorim Lima
OrcID
Polinar Bandeira Rufino
OrcID
Marcos Antônio de Oliveira Souza
OrcID
Genilda de Andrade Barbosa
OrcID
Jorgiane de Araújo Freitas
OrcID
Janis Lunier de Souza
OrcID
Pedro Junior Pinheiro Mourão
OrcID
Ricardo da Costa Rocha
OrcID
Emmerson Côrrea Brasil da Costa
OrcID

    Ellen Caroline Nobre Santos

    Federal University of Acre

    OrcID https://orcid.org/0000-0002-1467-1919

    Graduated in Biomedicine from Faculdade Barão de Rio Branco in 2017, I completed my master's degree in Science, Innovation and Technology at the Federal University of Acre. During this stage, I focused my work on researching larvicidal activity in ethanolic extracts of plants cultivated in the Western Amazon against Aedes aegypti. I am currently enrolled in the doctoral program in Biochemistry and Immunology at the Federal University of Minas Gerais, where my research focuses in invertebrate immunology, with emphasis on the small interfering RNA (siRNA) pathway in response to arbovirus infection.

    Taynara Lopes de Araújo

    Federal University of Acre

    OrcID https://orcid.org/0000-0002-0063-4981

    Graduated in Biological Sciences from the Federal University of Acre (UFAC). She was a scientific initiation student and has experience with teaching through the Pedagogical Residency and the Institutional Teaching Initiation Scholarship Program (PIBID).

    Andson Amorim Lima

    Federal University of Acre

    OrcID https://orcid.org/0000-0001-6770-2793

    Full Degree in Biological Sciences from the Federal University of Acre (UFAC).

    Polinar Bandeira Rufino

    Federal University of Acre

    OrcID https://orcid.org/0000-0002-3711-9481

    Academic training in Biomedicine and Biological Sciences from Centro Universitário - UNIMETA and Centro Universitário Leonardo da Vinci -UNIASSELVI, Rio Branco - AC, respectively. Master's degree from the Postgraduate Program in Health Sciences in the Western Amazon, with an emphasis on Molecular Genetics from the Federal University of Acre, Brazil. Specialist in Molecular and Serological Diagnosis in Clinical Analysis, from the University Center - UNINORTE, and Inclusive Special Education, Pedagogical Coordination and School Administration, supervision and guidance, from the Leonardo da Vinci University Center - UNIASSELVI, Rio Branco - AC. He worked as a PIBIC Fellow - FAPAC/PIBIC, for a period of 2 years, at the Brazilian Agricultural Research Corporation (Embrapa), working in the Morphogenesis and Molecular Biology Laboratory; and Director of Primary Care at the Department of Health and Basic Sanitation, in the municipality of Plácido de Castro, for a period of 6 months. Currently teaching Science and Physics, at the Acre Department of Education (SEE).

    Marcos Antônio de Oliveira Souza

    Federal University of Acre

    OrcID https://orcid.org/0000-0002-1450-6489

    Currently a full undergraduate student of the Degree in Biological Sciences at the Federal University of Acre (UFAC), with participation in the Institutional Teaching Initiation Scholarship Program and experience in the Cytogenetics and Molecular Genetics Laboratory, in addition to being a participating member of that laboratory . Scientific Initiation Student in the field of Genetics. Volunteer at the Cazumbá-Iracema extractive reserve.

    Genilda de Andrade Barbosa

    Federal University of Acre

    OrcID https://orcid.org/0000-0002-4550-4689

    Currently a PhD student at the PPG in Public Health - UFAC, training in Biomedicine at Centro Universitario Uninorte Rio Branco, Physical Education Bachelor's degree and degree at the Federal University of Acre UFAC; Specialist in Anatomy/physiology and Fundamental Biochemistry, Master Professor at Centro Universitario Uninorte and the Department of Education of the State of Acre, Registered with the Regional Council of Physical Education Bachelor/degree Nº625-G/Ac. Experience and Field of Activity: Master Professor at Centro Universitario Uninorte Disciplines Supervised Internship and Clinical Biochemistry in the Biomedicine and Pharmacy courses.

    Jorgiane de Araújo Freitas

    Federal University of Acre

    OrcID https://orcid.org/0000-0002-2892-6716

    She has a degree in Nursing from the Federal University of Acre (2014) and a degree in Biological Sciences from the Federal University of Acre (2009). Master's credits completed - Science, Innovation and Technology for the Amazon. Federal University of Acre.2017 to 2019. Specialist in Teaching in Professional, Technical and Technological Education. Federal Institute of Goiás. 2021 to 2023. Studying Specialization in Women's Health. Venda Nova do Imigrante College - FAVENI. 2023. Currently works as a teacher at Colégio Boas Novas. Goiânia.

    Janis Lunier de Souza

    Municipal Health Department of Rio Branco

    OrcID https://orcid.org/0000-0002-8836-9456

    He has a degree in Physical Education from the Federal University of Acre (2001) and a technical-professional course in Mid-Level Technician in Health Surveillance from the State Institute for the Development of Professional Education Dom Moacyr (2013). He is currently an Endemic Diseases Agent at the Municipal Health Department of Rio Branco. He has experience in the area of Parasitology, with an emphasis on Entomology and Malacology of Parasites and Vectors.

    Pedro Junior Pinheiro Mourão

    Federal University of Acre

    OrcID https://orcid.org/0000-0003-0683-2484

    PhD in Genetics from the Postgraduate Program in Genetics at the Federal University of Rio de Janeiro (2022) with a focus on HIV-1 reactivating agents. Master of Science from the postgraduate program in Science, Innovation and Technology (CITA) at the Federal University of Acre (2018) with a focus on the area of Molecular Genetics. Graduated in Biological Sciences from the Federal University of Acre (2015). He worked in the areas of Molecular Virology with a focus on work with the antiviral activity of natural products against HIV, Dengue, Zika and Chikungunya viruses. He currently works as a postdoc at the Molecular Virology Laboratory (LVM-UFRJ) working on investigating improvements for the use of ZIKV as an oncolytic virus for the treatment of glioblastoma. He also develops partner projects focusing on the characterization of the Oropouche and Mayaro viruses.

    Ricardo da Costa Rocha

    Federal University of Acre

    OrcID https://orcid.org/0000-0003-1320-7935

    He holds a Bachelor's degree and a Degree in Social Sciences, a Master's degree in Political Science from UFF - Universidade Federal Fluminense and a PhD in Social Service (Area: Social Policy and Labor) from UERJ - State University of Rio de Janeiro. He attended the Lato Sensu Postgraduate Specialization in African and Black Brazilian History at UCAM-Universidade Cândido Mendes. He worked as a teacher, researcher and technical advisor at Macaé City Hall (2004-2008). He served as a substitute professor, in various disciplines, at the School of Social Service of the Federal University of Rio de Janeiro (1998-1999), at the Faculty of Law of UERJ - State University of Rio de Janeiro (1999) and at the Faculty of Social Service at the Institute of Society Sciences and Regional Development - ESR, in Campos dos Goytacazes, RJ, at UFF - Universidade Federal Fluminense (2007-2008). He was a professor of Sociology at the Escola Técnica Estadual República, part of the FAETEC network - Foundation for Support to the Technical School of the State of Rio de Janeiro, between 1999 and 2008. He is a professor of Sociology at IFRJ - Federal Institute of Education, Science and Technology of Rio de Janeiro. Janeiro - Campus Arraial do Cabo since February 2020. Previously, he taught the same subject at IFRJ - Campus São Gonçalo (2009-2019), where he has been a teacher since 2011 of the Postgraduate Lato Sensu Specialization in Teaching African and Afro Histories and Cultures -Brazilians, also coordinating the course (2018-2020). Since 2017, he has coordinated the Education, Cultures and Decoloniality Studies and Research Group (GEPECD), being leader, together with CNPq, of the Cultures and Decoloniality Research Group, recognized and institutionally registered by the IFRJ. He is one of the founders of ABECS-Brazilian Association for Teaching Social Sciences, where he was a member of the Supervisory Board for two terms (2016-2020).

    Emmerson Côrrea Brasil da Costa

    Federal University of the State of Rio de Janeiro

    OrcID https://orcid.org/0000-0002-1467-1919

    He has a degree in Biological Sciences (Medical Modality) (Biomedicine) from the Federal University of the State of Rio de Janeiro (2002), a master's degree in Biological Sciences (Genetics) from the Federal University of Rio de Janeiro (2005) and a doctorate in Biological Sciences (Biophysics) from the University Federal Government of Rio de Janeiro (2009). He completed his post-doctorate at the Federal University of Rio de Janeiro (2012) in the area of enzyme kinetics and served as an adjunct professor at the Center for Health and Sports Sciences at the Federal University of Acre (2014-2021). He is currently an assistant professor in the Department of Genetics and Molecular Biology at the Federal University of the State of Rio de Janeiro (2022) and has experience in the area of molecular genetics, working mainly on the following topics: cancer genetics, polymorphism, purification of heterologous proteins, enzyme kinetics , molecular cloning, molecular diagnosis of arboviruses, molecular and structural biology.

Keywords

Aedes
Plants
Insecticides
Biochemistry
Arbovirus infections

Abstract

Aedes aegypti is the main transmitter of several arboviruses, such as dengue, the world's most widespread arbovirus. The main method of controlling arbovirus transmission is through vector control, using insecticides. But its continuous, cases of resistance occur. Thus, research is seeking new botanical insecticide alternatives, because have multiple active compounds can be applied on the vector, contributing to the control of arbovirus transmission. The aim of this study is to identify plants cultivated in the Western Amazon, which have larvicidal activity against A. aegypti. Ethanolic extracts from ten plants were obtained to verify the larvicidal biopotential against third instar larvae of A. aegypti. The promising ethanolic extracts was Fridericia chica, which was submitted to liquid-liquid partition for larvicidal activity evaluation and phytochemical analysis. Among the partitions analyzed, the aqueous partition of F. chica showed larvicidal biopotential against A. aegypti, being the first report in the literature of its larvicidal activity. In the phytochemical prospection of the aqueous partition of F. chica, we observed the presence of catechins, condensed tannins and the flavone group, flavonols and xanthones. These groups of metabolites have been reported in the literature as larvicidal agents.

References

  1. Rufino BP, Santos ECN, Maggi EL, Costa ECB. Mayaro Virus: an Emerging Arbovirosis in Brazil? Multidiscip Sci Reports. 2022; 2: 1–24. [https://doi.org/10.54038/ms.v2i2.19].
  2. Souza-Neto JA, Powell JR, Bonizzoni M. Aedes aegypti vector competence studies: a review. Infect Genet Evol. 2019; 67: 191–209. [https://doi.org/10.1016/j.meegid.2018.11.009].
  3. Santos ECN, Barbosa GA, Rufino PB, Lima AA, Araújo TL, Costa ECB. Zika Vírus: dos aspectos epidemiológicos ao tratamento. In: Saúde da criança e adolescente: epidemiologia, doenças infecciosas e parasitárias. 2019. p. 88–106. [https://doi.org/10.35170/ss.ed.9786580261109.06].
  4. Leta S, Beyene TJ, Clercq EM De, Amenu K, Revie CW, Kraemer MUG. Global risk mapping for major diseases transmitted by Aedes aegypti and Aedes albopictus. Int J Infect Dis. 2018; 67: 25–35. [https://doi.org/10.1016/j.ijid.2017.11.026].
  5. Patterson J, Sammon M, Garg M. Dengue, Zika and Chikungunya: emerging arboviruses in the new world. West J Emerg Med. 2016; 17: 671–9. [https://doi.org/10.5811/westjem.2016.9.30904].
  6. Rückert C, Weger-Lucarelli J, Garcia-Luna SM, Young MC, Byas AD, Murrieta RA et al. Impact of simultaneous exposure to arboviruses on infection and transmission by Aedes aegypti mosquitoes. Nat Commun. 2017; 8. [https://doi.org/10.1038/ncomms15412].
  7. Siqueira JB, Martelli CMT, Coelho GE, Simplício ACR, Hatch DL. Dengue and dengue hemorrhagic fever, Brazil, 1981-2002. Emerg Infect Dis. 2005; 11: 48–53. [https://doi.org/10.3201/eid1101.031091].
  8. Mlakar J, Korva M, Tul N, Popović M, Poljšak-Prijatelj M, Mraz J et al. Zika Virus Associated with Microcephaly. N Engl J Med. 2016; 374: 951–8. [https://doi.org/10.1056/NEJMoa1600651].
  9. Schuler-Faccini L, Ribeiro EM, Feitosa IML, Horovitz DDG, Cavalcanti DP, Pessoa A et al. Possible Association Between Zika Virus Infection and Microcephaly — Brazil, 2015. MMWR Morb Mortal Wkly Rep. 2016; 65: 1–4. [https://doi.org/10.15585/mmwr.mm6503e2er].
  10. Brasil P, Sequeira PC, Freitas ADA, Zogbi HE, Calvet GA, Souza RV et al. Guillain-Barré syndrome associated with Zika virus infection. Lancet [Internet]. 2016; 387: 1482. Available from: [https://doi.org/10.1016/S0140-6736(16)30058-7].
  11. Cao-Lormeau VM, Blake A, Mons S, Lastère S, Roche C, Vanhomwegen J et al. Guillain-Barré Syndrome outbreak associated with Zika virus infection in French Polynesia: A case-control study. Lancet. 2016; 387: 1531–9. [https://doi.org/10.1016/S0140-6736(16)00562-6].
  12. Sales GMPG, Barbosa ICP, Neta LMSC, Melo PL, Leitão RA, Melo HMA. Treatment of Chikungunya chronic arthritis: a systematic review. Rev Assoc Med Bras. 2018; 64: 63–70. [https://doi.org/10.1590/1806-9282.64.01.63].
  13. Schilte C, Staikovsky F, Couderc T, Madec Y, Carpentier F, Kassab S et al. Chikungunya Virus-associated Long-term Arthralgia: A 36-month Prospective Longitudinal Study. PLoS Negl Trop Dis. 2013; 7 (3):e2137. [https://doi.org/10.1371/journal.pntd.0002137] [https://pubmed.ncbi.nlm.nih.gov/23556021/] .
  14. Yang Y, Meng Y, Halloran ME, Longini IM. Dependency of Vaccine Efficacy on Preexposure and Age: A Closer Look at a Tetravalent Dengue Vaccine. Clin Infect Dis. 2018; 66: 178–84. [https://doi.org/10.1093/cid/cix766].
  15. White LJ, Young EF, Stoops MJ, Henein SR, Adams EC, Baric RS et al. Defining levels of dengue virus serotype-specific neutralizing antibodies induced by a live attenuated tetravalent dengue vaccine (Tak-003). PLoS Negl Trop Dis [Internet]. 2021; 15: 1–15. Available from: [http://dx.doi.org/10.1371/journal.pntd.0009258].
  16. Carvalho FD, Moreira LA. Why is Aedes aegypti Linnaeus so Successful as a Species? Neotrop Entomol. 2017; 46: 243–55.[https://doi.org/10.1007/s13744-017-0520-4].
  17. Rodríguez MM, Bisset JA, Fernández D. Levels of insecticide resistance and resistance mechanisms in Aedes aegypti from some Latin American countries. J Am Mosq Control Assoc. 2007; 23: 420–9. [https://doi.org/10.2987/5588.1].
  18. Carvalho MSL, Caldas ED, Degallier N, Vilarinhos PTR, Souza LCKR, Yoshizawa MAC et al. Susceptibility of Aedes aegypti larvae to the insecticide temephos in the Federal District, Brazil. Rev Saude Pública. 2004; 38: 623–9. [https://doi.org/10.1590/S0034-89102004000500002].
  19. Da-Cunha MP, Lima JBP, Brogdon WG, Moya GE, Valle D. Monitoring of resistance to the pyrethroid cypermethrin in Brazilian Aedes aegypti (Diptera Culicid. Mem Inst Oswaldo Cruz. 2005; 100: 441–4. [https://doi.org/10.1590/S0074-02762005000400017].
  20. Braga IA, Lima JBP, Soares SS, Valle D. Aedes aegypti resistance to temephos during 2001 in several municipalities in the states of Rio de Janeiro, Sergipe, and Alagoas, Brazil. Mem Inst Oswaldo Cruz. 2004; 99: 199–203. [https://doi.org/10.1590/S0074-02762004000200015].
  21. Moyes CL, Vontas J, Martins AJ, Ng LC, Koou SY, Dusfour I et al. Contemporary status of insecticide resistance in the major Aedes vectors of arboviruses infecting humans. PLoS Negl Trop Dis. 2017; 11: e0005625. [https://doi.org/10.1371/journal.pntd.0005625].
  22. De Araújo AP, Paiva MHS, Cabral AM, Cavalcanti AEHD, Pessoa LFF, Diniz DFA et al. Screening Aedes aegypti (Diptera: Culicidae) Populations from Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms. J Insect Sci. 2019; 19: 1–15. [https://doi.org/10.1093/jisesa/iez054].
  23. Ciccia G, Coussio J, Mongelli E. Insecticidal activity against Aedes aegypti larvae of some medicinal South American plants. J Ethnopharmacol. 2000; 72: 185–9. [https://doi.org/10.1016/S0378-8741(00)00241-5].
  24. Pohlit AM, Quinard ELJ, Nunomura SM, Tadei WP, Hidalgo AF, Pinto ACS et al. Screening of plants found in the State of Amazonas, Brazil for activity against Aedes aegypti larvae. Acta Amaz. 2004; 34: 97–105. [https://doi.org/10.1590/S0044-59672004000100012].
  25. Omena MC, Navarro DMAF, Paula JE, Luna JS, Lima MRF, Sant’Ana AEG. Larvicidal activities against Aedes aegypti of some Brazilian medicinal plants. Bioresour Technol. 2007; 98(13): 2549–56. [https://doi.org/10.1016/j.biortech.2006.09.040].
  26. Ghosh A, Chowdhury N, Chandra G. Plant extracts as potential mosquito larvicides. Indian J Med Res. 2012; 135(5): 581–98. PMCID: PMC3401688. PMID: 22771587. [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401688/].
  27. Rodrigues AM, Sampaio CG, Souza JSN, Campos AR, Silva ABR, Morais SM et al. Different susceptibilities of Aedes aegypti and Aedes albopictus larvae to plant-derived products. Rev Soc Bras Med Trop. 2019; 52. [https://doi.org/10.1590/0037-8682-0197-2018].
  28. Araújo IF, Araújo PHF, Ferreira RMA, Sena IDS, Lima AL, Carvalho JCT et al. Larvicidal effect of hidroethanolic extract from the leaves of Acmella oleracea L. R. K. Jansen in Aedes aegypti and Culex quinquefasciatus. South African J Bot. 2018; 117: 134–40. [https://doi.org/10.1016/j.sajb.2018.05.008].
  29. Anunciação SCM. Ovitrampa sem acúmulo de água: metodologia segura para coleta de ovos de Aedes? Rio de Janeiro, 2017. 25 f. Trabalho de Conclusão de Curso, Especialização em Entomologia Médica [Programa de Pós-Graduação em Entomologia Médica] - Instituto Oswaldo Cruz. Fundação Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. 2017. [https://www.arca.fiocruz.br/handle/icict/34796].
  30. Consoli RAGB, Oliveira RL. Principais mosquitos de importância sanitária no Brasil. Editora Fiocruz. Rio de Janeiro; 1994. 228 p. [https://doi.org/10.7476/9788575412909].
  31. Costa-da-Silva AL, Navarrete FR, Salvador FS, Karina-Costa M, Ioshino RS, Azevedo DS et al. Glytube: A Conical Tube and Parafilm M-Based Method as a Simplified Device to Artificially Blood-Feed the Dengue Vector Mosquito, Aedes aegypti. PLoS One. 2013; 8: e53816. [https://doi.org/10.1371/journal.pone.0053816].
  32. World Health Organization. Guidelines for laboratory and field testing of mosquito larvicides. World Heal Organ. 2005; 1–41. [https://www.who.int/publications/i/item/WHO-CDS-WHOPES-GCDPP-2005.13].
  33. Vidal J, Carbajal A, Sisniegas M, Bobadilla M. Efecto tóxico de Argemone subfusiformis Ownb. y Tagetes patula Link sobre larvas del IV estadio y pupas de Aedes aegypti L. Rev Peru Biol. 2008; 15: 103–9. [https://doi.org/10.15381/rpb.v15i2.1733].
  34. Macêdo ME, Consoli RAGB, Grandi TSM, Anjos AMG, Oliveira AB, Mendes NM et al. Screening of Asteraceae (Compositae) Plant Extracts for Larvicidal Activity against Aedes fluviatilis (Diptera: Culicidae). Mem Inst Oswaldo Cruz. 1997; 92: 565–70. [https://doi.org/10.1590/S0074-02761997000400024].
  35. Matos JFA. Introdução à Fitoquímica Experimental. 3ª ed. Fortaleza: Editora UFC; 2009. 150 p.
  36. Rahuman AA, Gopalakrishnan G, Venkatesan P, Geetha K. Larvicidal activity of some Euphorbiaceae plant extracts against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res. 2008; 102: 867–73. [https://doi.org/10.1007/s00436-007-0839-6].
  37. Musau J, Mbaria J, Nguta J, Mathiu M, Kiama S. Phytochemical composition and larvicidal properties of plants used for mosquito control in Kwale County, Kenya. Int J Mosq Res. 2016; 3: 12–7. [http://erepository.uonbi.ac.ke/handle/11295/100694].
  38. Pandey A, Tripathi S. Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. J Pharmacogn Phytochem. 2014; 2(5): 115–9. E-ISSN: 2278-4136. [https://www.phytojournal.com/archives/2014.v2.i5.255/concept-of-standardization-extraction-and-pre-phytochemical-screening-strategies-for-herbal-drug].
  39. Violante IMP, Carollo CA, Silva LI, Oliveira AQC, Pardinho FC, Garcez WS et al. Cytotoxicity and antibacterial activity of scutellarein and carajurone-enriched fraction obtained from the hydroethanolic extract of the leaves of Fridericia chica (Bonpl.) L.G. Lohmann. Nat Prod Res. 2020; 1-7.[https://doi.org/10.1080/14786419.2020.1753050]
  40. Lima JCS, Oliveira RG, Silva VC, Sousa PT, Violante IMP, Macho A et al. Anti-inflammatory activity of 4′,6,7-trihydroxy-5-methoxyflavone from Fridericia chica (Bonpl.) L.G.Lohmann. Nat Prod Res. 2020; 34: 726–30. [https://doi.org/10.1080/14786419.2018.1495636].
  41. Generoso LA, Araujo PF, Cecílio AB. Avaliação do efeito do óleo essencial da Fridericia chica em diferentes fases de vida do mosquito Aedes aegypti. Rev Sinapse Múltipla. 2020; 9: 47–60. [https://periodicos.pucminas.br/index.php/sinapsemultipla/article/view/21107].
  42. Silva HHG, Silva IG, Santos RMG, Rodrigues Filho E, Elias CN. Larvicidal activity of tannins isolated of Magonia pubescens St. Hil. (Sapindaceae) against Aedes aegypti (Diptera, Culicidae). Rev Soc Bras Med Trop. 2004; 37: 396–9. [https://doi.org/10.1590/s0037-86822004000500005] [https://pubmed.ncbi.nlm.nih.gov/15361956/].
  43. Raihan SMA. Effect of Plant Flavonoids on Mosquito Larvae. Natl Univ J Sci. 2014; 1(2): 27–30. [chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://www.nu.ac.bd/uploads/2017/09/03.-EFFECT-OF-PLANT-FLAVONOIDS-ON-MOSQUITO-LARVAE.pdf].
  44. Elumalai D, Hemavathi M, Hemalatha P, Deepaa CV, Kaleena PK. Larvicidal activity of catechin isolated from Leucas aspera against Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res. 2016; 115: 1203–12. [https://doi.org/10.1007/s00436-015-4856-6].
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Identification of larvicidal activity of medicinal plants against Aedes aegypti (Diptera: Culicidae). Rev Fitos [Internet]. 2024 Jun. 24 [cited 2024 Nov. 23];18:e1619. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1619
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