Establishment of anthocyanin-producing habituated callus cultures of Tarenaya rosea (Vahl ex DC.) Soares Neto & Roalson

Drª Claudia Simões-Gurgel
OrcID
Drª Lívia da Silva Cordeiro
OrcID
Drª Tatiana Carvalho de Castro
OrcID
Drª Norma Albarello
OrcID

    Drª Claudia Simões-Gurgel

    Universidade do Estado do Rio de Janeiro

    OrcID https://orcid.org/0000-0001-8358-2568

    Possui Graduação em Ciências Biológicas pela Universidade do Estado do Rio de Janeiro, Mestrado em Biologia (Biociências Nucleares) pela Universidade do Estado do Rio de Janeiro e Doutorado em Biotecnologia Vegetal pela Universidade Federal do Rio de Janeiro. Bióloga do Instituto de Biologia Roberto Alcantara Gomes (IBRAG) da Universidade do Estado do Rio de Janeiro, Docente do Corpo Permanente do Programa de Pós-Graduação em Biologia Vegetal (PGBV/UERJ) e pesquisadora do Núcleo de Biotecnologia Vegetal (NBV/UERJ). Experiência nas áreas de Fisiologia Vegetal e Biotecnologia Vegetal, com ênfase em Cultura de Tecidos, atuando principalmente nos seguintes temas: criopreservação, produção in vitro de plantas e substâncias bioativas.

    Drª Lívia da Silva Cordeiro

    Universidade do Estado do Rio de Janeiro

    OrcID https://orcid.org/0000-0002-6964-7000

    Possui graduação em Ciências Biológicas, licenciatura (2007) e bacharelado (2010), pela Universidade do Estado do Rio de Janeiro, mestrado em Biologia Vegetal (2011) pela Universidade do Estado do Rio de Janeiro e doutorado em Biologia Vegetal pela Universidade do Estado do Rio de Janeiro, com sanduíche no Institut de Recherche pour le Développement (IRD), França (2016). Tem experiência nas áreas de Fisiologia Vegetal e Biotecnologia Vegetal, atuando principalmente nos seguintes temas: plantas medicinais, cultura de tecidos vegetais, criopreservação, produção de metabólitos in vitro, marcadores moleculares e fitoquímica.

    Drª Tatiana Carvalho de Castro

    State University of Rio de Janeiro

    OrcID https://orcid.org/0000-0003-0554-6873

    He has a technical course in food chemistry from the Federal Technical School of Chemistry (1991), a degree in Pharmacy from the Federal University of Rio de Janeiro (1997), a master's degree in Biological Sciences (Nuclear Biosciences) from the State University of Rio de Janeiro (2000) and a PhD in Plant Biology from the State University of Rio de Janeiro (2015). She is currently a Pharmacist (Profile-Natural Products) at the State University of Rio de Janeiro and a Collaborating Researcher at the Oswaldo Cruz Foundation. She has experience in the area of ​​Plant Biotechnology, working mainly on the following topics: production of in vitro metabolites, phytochemical profile, Cleomaceae and Rhamnaceae.

    Drª Norma Albarello

    State University of Rio de Janeiro

    OrcID https://orcid.org/0000-0001-5803-2070

    Graduated in Biological Sciences from the State University of Rio de Janeiro - UERJ, with a Master's degree in Biological Sciences, Area of ​​Concentration in Botany, from the Federal University of Rio de Janeiro - UFRJ and PhD in Biology, Area of ​​Concentration in Nuclear Biosciences, from the University of the State of Rio de Janeiro - UERJ. Associate Professor and Proscientist at the Department of Plant Biology (DBV/IBRAG/UERJ), where she served as head and deputy head between 2006 and 2008. Professor of the Postgraduate Program in Plant Biology (PGBV/IBRAG/UERJ) where she served as Coordinator Deputy in the 2008-2010 biennium. Deputy director of the Roberto Alcantara Gomes Biology Institute - IBRAG/UERJ, for two terms (2012 to 2019). Elected director of IBRAG/UERJ (2020-2023; 2024-2027). Coordinator of the Plant Biotechnology Laboratory (Labplan), Technological Development Unit - UDT of the Plant Biotechnology Center at UERJ (Group leader/CNPq). He served on the Superior Councils of UERJ (Csepe and Consun). She is a member of the UERJ Technical Innovation Chamber. Experience in the areas of Plant Physiology and Plant Biotechnology, with an emphasis on tissue culture, working mainly on the following topics: medicinal plants, natural products, germination, micropropagation, secondary metabolites, in vitro production and ex situ conservation.

Keywords

Cleome rosea
habituation
MS medium
NH4+:NO3-ratio
Total nitrogen
Sucrose

Abstract

A growth regulators-free anthocyanin-producing habituated callus line of Tarenaya rosea was establishment through the selective subculture of cell aggregates from a 2,4-D-dependent anthocyanin callus line to a growth regulators-free MS medium (MS0). After the establishment of the habituated line, new culture conditions were evaluated in order to increase de pigment production. Calluses were transferred to MS0 containing different sucrose concentrations (30; 50; 70; 90 g.L-1), total nitrogen concentrations (50; 60; 70 mM), NH4+ to NO3- ratios (1:1; 1:2; 1:4; 1:6), and total mineral salt concentration (MS; MS1/2; MS1/4). The most suitable culture conditions to pigment induction have combined to create two new culture formulations, named M1 (70 mM total nitrogen + 70 g.L-1 sucrose) and M2 (1:4 NH4+/NO3- ratio + 70 g.L-1 sucrose). Calluses cultivated on M1 and M2 reached an increase on anthocyanin productivity of 3- and 4-fold, respectively, when compared to cultures maintained in standard MS0 medium. The present work demonstrated the feasibility of eliminating the supplementation with 2,4-D in callus cultures of T. rosea without the loss of anthocyanin production. Moreover, the manipulations of basal medium and sucrose concentration contributed with the increment in anthocyanin content.

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Establishment of anthocyanin-producing habituated callus cultures of Tarenaya rosea (Vahl ex DC.) Soares Neto & Roalson . Rev Fitos [Internet]. 2024 Jul. 19 [cited 2024 Nov. 21];18:e1662. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1662
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