Evaluation of toxicity, cytotoxicity and genotoxicity of infusion of rhizomes of Curcuma longa L. (Zingiberaceae)

Leonardo Mendes da Silva
OrcID
Franciele Filardi Cimino
OrcID
Ana Letícia Borgo
OrcID
Vanessa de Souza Vieira Dutra
OrcID
José Emílio Zanzirolani de Oliveira
OrcID

    Leonardo Mendes da Silva

    Federal University of Lavras (UFLA), Department of Ecology, Laboratory of Ecogenotoxicology and Cytogenetics, Praça Prof. Edmir Sá Santos, s/n - Aquenta Sol, CEP 37200-900, Lavras, MG, Brazil

    OrcID https://orcid.org/0000-0001-6510-9005

    Master's student in Applied Botany at the Federal University of Lavras (UFLA). Post-Graduate in Ecology and Biodiversity at the Faculty of Administration, Sciences and Education (FAMART). Degree in Biological Sciences from the Federal Institute of the Southeast of Minas Gerais - Campus Barbacena (2018-2022). He works as a researcher in the field of botany and ecology, using plant test organisms in order to assess the toxicity, cytotoxicity, genotoxicity and mutagenicity of environmental pollutants and plant-derived extracts.

    Franciele Filardi Cimino

    Federal University of Viçosa (UFV), Department of General Biology, Laboratory of Applied Cancer Research (LAPAC), Av. P. H. Rofls, s/nº, Campus Universitário, CEP 36570-900, Viçosa, MG, Brazil

    OrcID https://orcid.org/0000-0002-8755-2793

    He holds a degree in Biological Sciences from the Federal Institute of the Southeast of Minas Gerais (2018) and a master's degree in Cellular and Structural Biology from the Federal University of Viçosa (2021). She is currently a doctoral student at the Federal University of Viçosa. She has experience in General Biology, with emphasis on General Biology, working mainly on the following topics: cytogenetics, chemotherapeutics, cancer and cell culture.

    Ana Letícia Borgo

    Federal Institute of Education, Science and Technology of Southeast Minas Gerais, Interdisciplinary Laboratory for Educator Training (LIFE), Barbacena campus, Rua Monsenhor José Augusto 204, São José, CEP 36205-018, Barbacena, MG, Brazil

    OrcID https://orcid.org/0000-0003-2432-1223

    Completed high school in 2017 and is currently pursuing a degree in Biological Sciences at the Federal Institute of Education, Science and Technology of the Southeast of Minas Gerais - Campus Barbacena, as well as a scholarship holder and resident in the Pedagogical Residence project.

    Vanessa de Souza Vieira Dutra

    Federal Institute of Education, Science and Technology of Southeast Minas Gerais, Interdisciplinary Laboratory for Educator Training (LIFE), Barbacena campus, Rua Monsenhor José Augusto 204, São José, CEP 36205-018, Barbacena, MG, Brazil

    OrcID https://orcid.org/0000-0002-2325-2492

    Graduate student of the Degree in Biological Sciences at the Federal Institute of Education, Science and Technology of the Southeast of Minas Gerais - Campus Barbacena, in which she was a scholarship holder of the Institutional Teaching Initiation Scholarship Program (Pibid) and is currently a member of the Pedagogical Residency Program .

    José Emílio Zanzirolani de Oliveira

    Federal Institute of Education, Science and Technology of Southeast Minas Gerais, Interdisciplinary Laboratory for Educator Training (LIFE), Barbacena campus, Rua Monsenhor José Augusto 204, São José, CEP 36205-018, Barbacena, MG, Brazil.

    OrcID https://orcid.org/0000-0002-0008-4561

    He holds a degree in Biological Sciences from the Federal University of Juiz de Fora (1994), a degree in Sciences from the Faculty of Philosophy, Sciences and Letters of Cataguases (1990), a Master's degree in Genetics and Improvement from the Federal University of Viçosa (1997) and a PhD in Genetics. and Improvement by the Federal University of Viçosa (2001). Currently he is Prof. Basic, Technical and Technological Education at the Federal Institute of the Southeast of Minas Gerais. He has experience in the field of Genetics. Acting mainly on the following topics: Bidens pilosa, Medicinal plants, anatomy, isozymes, altitude and phenotypic plasticity.

Keywords

Turmeric
Allium cepa
Cytogenetics analysis

Abstract

Curcuma longa L. (Zingiberaceae) saffron is originally from Southeast Asia has rhizomes containing curcumin that confer medicinal and flavoring uses. In the present study the toxic cytotoxic and genotoxic effects of Curcuma longa L. rhizome infusion on the Allium cepa plant test system was evaluated. The infusion of crushed rhizome was used in three concentrations: 10, 20, 40 mg.mL-1, in addition to a negative control (distilled water) and a positive control (glyphosate).  Toxicity effects were obtained by the average length of roots. The cytotoxic and genotoxic aspects were analyzed microscopically at any stage of the cell cycle, using the mitotic index and the chromosomal aberration index, respectively. The data were subjected to analysis of variance and means were compared by the Dunnett 5% test. It was observed that the concentration of 10 mg.mL-1 is safe. The concentration of 40 mg.mL-1 was toxic for significantly inhibiting the root growth of the bulbs. At 20 and 40 mg.mL-1 concentrations there was antiproliferative with a significant reduction in the mitotic index. Furthermore, at a concentration of 20 mg.mL-1 the presence of binucleated cells was observed and at 40 mg.mL-1 delayed chromosomes binucleate cells and telophasic bridge were observed.

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Author(s)

  • Leonardo Mendes da Silva
    Federal University of Lavras (UFLA), Department of Ecology, Laboratory of Ecogenotoxicology and Cytogenetics, Praça Prof. Edmir Sá Santos, s/n - Aquenta Sol, CEP 37200-900, Lavras, MG, Brazil
    https://orcid.org/0000-0001-6510-9005
  • Franciele Filardi Cimino
    Federal University of Viçosa (UFV), Department of General Biology, Laboratory of Applied Cancer Research (LAPAC), Av. P. H. Rofls, s/nº, Campus Universitário, CEP 36570-900, Viçosa, MG, Brazil
    https://orcid.org/0000-0002-8755-2793
  • Ana Letícia Borgo
    Federal Institute of Education, Science and Technology of Southeast Minas Gerais, Interdisciplinary Laboratory for Educator Training (LIFE), Barbacena campus, Rua Monsenhor José Augusto 204, São José, CEP 36205-018, Barbacena, MG, Brazil
    https://orcid.org/0000-0003-2432-1223
  • Vanessa de Souza Vieira Dutra
    Federal Institute of Education, Science and Technology of Southeast Minas Gerais, Interdisciplinary Laboratory for Educator Training (LIFE), Barbacena campus, Rua Monsenhor José Augusto 204, São José, CEP 36205-018, Barbacena, MG, Brazil
    https://orcid.org/0000-0002-2325-2492
  • José Emílio Zanzirolani de Oliveira
    Federal Institute of Education, Science and Technology of Southeast Minas Gerais, Interdisciplinary Laboratory for Educator Training (LIFE), Barbacena campus, Rua Monsenhor José Augusto 204, São José, CEP 36205-018, Barbacena, MG, Brazil.
    https://orcid.org/0000-0002-0008-4561

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Evaluation of toxicity, cytotoxicity and genotoxicity of infusion of rhizomes of Curcuma longa L. (Zingiberaceae). Rev Fitos [Internet]. 2023 Mar. 31 [cited 2024 Nov. 21];17(1):9-17. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1447
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