A preliminary study of the cytotoxicity of the protein extract of abajerú commercialized in markets

Thaís de Almeida Pedrete
Julianderson de Oliveira dos Santos Carmo
Emiliano de Oliveira Barreto
Josino Costa Moreira

    Thaís de Almeida Pedrete

    State University of Rio de Janeiro (UERJ), Pavilhão Haroldo Lisboa da Cunha, Institute of Biology Roberto Alcântara Gomes, Rua São Francisco Xavier, 524, Maracanã, CEP 20550-900, Rio de Janeiro, RJ, Brazil.

    Graduated in Biological Sciences from Universidade Santa Úrsula (2010), Master in Chemistry from Pontifical Catholic University of Rio de Janeiro (2013) and PhD in Public Health and Environment from the School of Public Health - Fiocruz (2018). Has experience in Analytical Chemistry, with an emphasis on Monitoring and Environmental Chemistry, and in the area of ​​Public Health and Environment, with an emphasis on Molecular Biology. He worked as a coordination assistant at the Laboratory of Marine and Environmental Studies (LABMAM) at PUC-Rio, developing methodologies for cellular and genetic biomarkers (micronucleus, neutral red and comet assay) and analytical methods for determining PAH metabolites in LC / MS, HPLC and GC / MS, and served as a substitute professor in the Department of Oceanography at UERJ. During his doctorate, he developed methodologies and applications of potential proteomic markers in the study of human diseases and occupational exposure, in the Toxicology laboratory of the Center for the Study of Worker's Health and Human Ecology (CESTEH). He currently develops methods to identify and quantify protein biomarkers for neurodegenerative diseases at the Neurophysiology Laboratory - IBRAG / UERJ, as a PNPD / Capes fellow.

    Julianderson de Oliveira dos Santos Carmo

    Federal University of Alagoas (UFAL), Institute of Biological and Health Sciences, Laboratory of Cell Biology, campus A.C. Simões. Avenida Lourival Melo Mota, s / n, Tabuleiro dos Martins, CEP 57072-970, Maceió, AL, Brazil.

    Graduated in Biological Sciences from the Federal University of Alagoas (2018) and Master in Health Sciences from the Federal University of Alagoas (2019). Has experience in Pharmacology, with emphasis on Biochemical and Molecular Pharmacology, acting on the following subjects: endothelial cells, triterpenoids and angiogenesis.

    Emiliano de Oliveira Barreto

    Federal University of Alagoas (UFAL), Institute of Biological and Health Sciences, Laboratory of Cell Biology, campus A.C. Simões. Avenida Lourival Melo Mota, s / n, Tabuleiro dos Martins, CEP 57072-970, Maceió, AL, Brazil.

    Graduated in Biological Sciences from the Federal University of Rio de Janeiro (1997), master's (2001) and doctorate (2005) in Cellular and Molecular Biology from the Oswaldo Cruz Foundation. He completed a post-doctorate (2015) at the University of Rennes-1, an institution that maintains scientific cooperation in France. Associate Professor at the Federal University of Alagoas accredited in the Postgraduate Program in Health Sciences where he served as vice-coordinator (2009-2011) and coordinator (2011-2013). Member of the Brazilian Society of Pharmacology and Experimental Therapeutics (SBFTE), being elected to the Fiscal Council (2011-2014) and to the Deliberative Council (2014-2017) where he serves as President (2017-2020). Local coordinator of SIG-Farmaco (Special Interest Group in Pharmacology and Therapeutics at the University Telemedicine Network - RUTE). Coordinates and participates in projects in the field of pharmacology, cell biology and nanotechnology, acting mainly on the following topics: inflammation, nanobiotechnology and natural product.

    Josino Costa Moreira

    Oswaldo Cruz Foundation, National School of Public Health, Center for the Study of Workers' Health and Human Ecology (CESTEH), Rua Leopoldo Bulhões, 1480 Manguinhos, CEP 21041-210, Rio de Janeiro, RJ, Brazil.

    Graduated in Pharmacy from the Federal University of Juiz de Fora (1967), Master in Chemistry (Inorganic Analytical Chemistry) from the Pontifical Catholic University of Rio de Janeiro (1977) and doctorate in Chemistry - Loughborough University (1991). Has experience in Chemistry, with emphasis on Electroanalytical, acting on the following subjects: rio de janeiro, environmental contamination, voltammetry, lead and pesticides. & # 10; & # 10; Factor H = 25 (Scopus base, 2011)

Keywords

Cytotoxicity
Protein extract
Hypoglycemic plant
Chrysobalanus icaco
Eugenia astringens

Abstract

The use of plants and their products for medical treatment is a quite common procedure in Brazil, especially for treatment of diabetes. In fact, several plants can demonstrate hypoglycemic effects in vitro assays. However, the use for human treatment requires the knowledge of their toxicological properties. The aim of this study was to evaluate the effect of protein extracts of Chrysobalanus icaco collected from natural habitats and of Eugenia astringens acquired from the market in Rio de Janeiro on the viability and migration of fibroblasts. E. astringens has a similar morphology as C. icaco and it is sold as Chrysobalanus in a popular market. Being a different plant, E. astringens expresses different proteins, and its protein extract has proved to possess higher toxic properties than C. icaco does. Cytotoxicity assays indicated that, as the protein extract concentration increases, fibroblast viability decreases. Only the E. astringens extract displayed cytotoxicity at all concentrations, in addition to reduced fibroblast migration. The results obtained in this study demonstrates that it is necessary integrative policies for rational use of medicinal plants and their commercialization, since the current use of medicinal plants may be inadequate, and it is of great importance for Public Health.

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Author(s)

  • Thaís de Almeida Pedrete
    State University of Rio de Janeiro (UERJ), Pavilhão Haroldo Lisboa da Cunha, Institute of Biology Roberto Alcântara Gomes, Rua São Francisco Xavier, 524, Maracanã, CEP 20550-900, Rio de Janeiro, RJ, Brazil.
  • Julianderson de Oliveira dos Santos Carmo
    Federal University of Alagoas (UFAL), Institute of Biological and Health Sciences, Laboratory of Cell Biology, campus A.C. Simões. Avenida Lourival Melo Mota, s / n, Tabuleiro dos Martins, CEP 57072-970, Maceió, AL, Brazil.
  • Emiliano de Oliveira Barreto
    Federal University of Alagoas (UFAL), Institute of Biological and Health Sciences, Laboratory of Cell Biology, campus A.C. Simões. Avenida Lourival Melo Mota, s / n, Tabuleiro dos Martins, CEP 57072-970, Maceió, AL, Brazil.
  • Josino Costa Moreira
    Oswaldo Cruz Foundation, National School of Public Health, Center for the Study of Workers' Health and Human Ecology (CESTEH), Rua Leopoldo Bulhões, 1480 Manguinhos, CEP 21041-210, Rio de Janeiro, RJ, Brazil.

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A preliminary study of the cytotoxicity of the protein extract of abajerú commercialized in markets. Rev Fitos [Internet]. 2021 Mar. 31 [cited 2024 Nov. 21];15(1):11-2. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/897
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