In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract

Cristiane Pimentel Victorio
Davi Oliveira e Silva
Daniela Alviano
Celuta Alviano
Ricardo Machado Kuster
Celso Luiz Salgueiro Lage

    Cristiane Pimentel Victorio

    Centro Universitário Estadual da Zona Oeste (UEZO), Colegiado de Ciências Biológicas e da Saúde - CCBS. Avenida Manuel Caldeira de Alvarenga, 1203 Campo Grande, CEP 23070-200, Rio de Janeiro, RJ, Brasil.

    holds a degree in Biological Sciences from the State University of Rio de Janeiro (2000) and a master's and doctorate in Biological Sciences from the Biophysics Institute of the Federal University of Rio de Janeiro (2004/2008). In the area of ​​Education, she completed a post-graduate course (latu-sensu) talking about the use of trails in Botany Education, by UERJ (2002) and has projects for popularization and environmental education in schools in the city of Rio de Janeiro. She has experience in the field of Botany, with an emphasis on Plant and Environmental Biotechnology, working on the following topics: plant tissue culture, plant anatomy and physiology, phytochemistry and phytoremediation. During the master's degree, she did studies relating biophysical aspects, such as light, UV radiation and hormones and plant development, anatomy and phytochemistry. In the doctorate, he evaluated the production of special metabolites in vitro, using tissue culture and phytochemical techniques: flavonoids and essential oils, in addition to biological activities of plant extracts. She is currently an adjunct professor of Plant Biology and Biotechnology at UEZO-Rio de Janeiro and is a member of the teaching group of the Professional Master in Environmental Science and Technology.

    Davi Oliveira e Silva

    Universidade Federal de Ouro Preto (UFOP), Department of Biodiversity, Evolution and Environment-DEBIO, Institute of Exact and Biological Sciences (ICEB), Morro do Cruzeiro campus, CEP 35400-000, Ouro Preto, MG, Brazil.

    Graduated in Biological Sciences: Microbiology and Immunology from the Federal University of Rio de Janeiro-UFRJ (2006), MASTER in Plant Biotechnology - Decan of CCS - UFRJ (2009) and PhD in Plant Biotechnology - Decan of CCS - UFRJ (2013). He is currently a Biologist at the Federal University of Ouro Preto-UFOP where he is responsible for analysis in High Performance Liquid Chromatography (HPLC) of environmental samples from DEBIO-ICEB III, as well as monitoring maintenance of analytical equipment. Has experience in the area of Applied Microbiology, with emphasis on Research of new antimicrobial agents, acting mainly on the following themes: Cocos nucifera L., Aristolochia cymbifera, Plant Extracts, Essential Oil, Biological Activity, Phytochemical Analysis and Analysis of Environmental Samples.

    Daniela Alviano

    Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, s / n, 21941-902 Rio de Janeiro-RJ, Brazil

    Celuta Alviano

    Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, s / n, 21941-902 Rio de Janeiro-RJ, Brazil

    Ricardo Machado Kuster

    Federal University of Espírito Santo (UFES), Department of Chemistry, Av. Fernando Ferrari, 514, Goiabeiras, CEP 29075-910, Vitória, ES, Brazil

    Graduated from the Faculty of Pharmacy and Biochemistry of Espirito Santo (1987), master's degree in Chemistry of Natural Products from the Federal University of Rio de Janeiro (1991) and doctorate in Chemistry of Natural Products from the Federal University of Rio de Janeiro (1994). He is currently a professor and researcher in the Department of Chemistry at the Federal University of Espírito Santo, working in the research of natural products for application in the petroleum industry and chromatography of petroleum and derivatives.

    Celso Luiz Salgueiro Lage

    National Institute of Industrial Property (INPI), DICOD - Academia. Praça Mauá 7, Sala 1012, Centro, CEP 20081-240, Rio de Janeiro, RJ, Brazil

    Graduated in Physics from the Federal University of Rio de Janeiro (1984), Master's in Biological Sciences (Biophysics) from the Federal University of Rio de Janeiro (1989) and PhD in Biological Sciences (Biophysics) from the Federal University of Rio de Janeiro (1995) . Post-doctorate in the Department of Scientific and Technological Policy of the IG of UNICAMP. He is currently Senior Specialist in Industrial Property in Biotechnology at INPI. He has experience in Plant Biotechnology, with an emphasis on Tissue Culture, and Intellectual Property in Biotechnology.

Keywords

Antifungal activity
Zingiberaceae
Agar drop diffusion
Filamentous fungi
Yeast

Abstract

This study aimed to evaluate the in vitro antimicrobial activity of fractions of leaf hydroalcoholic crude extracts: hexane, dichloromethane, ethyl acetate and butanolic of Alpinia zerumbet and A. purpurata by the agar drop diffusion method in order to screen the main compounds involved in antimicrobial activity. Leaves of A. zerumbet (Pers.) B.L. Burtt et R.M. Sm. and A. purpurata (Vieill) K. Schum adult plants were collected and then dried and macerated in 70% ethanol. Leaf extracts were further partitioned using solvents in increasing polarity. Dichloromethane fractions were analyzed by gas chromatography/mass spectrometry (GC/MS). The major compound in the dichloromethane fraction of both species was the hexadecanoic acid (palmitic acid). By drop diffusion assay, the antimicrobial activity of crude extract, as well as fractions of hexane, dichloromethane, ethyl acetate and butanolic, was evaluated against pathogenic bacteria and fungi. No bacteria were inhibited. However, the dichloromethane fraction exhibited promising antifungal activity against the following fungi tested: Cryptococcus neoformans, Fonsecaea pedrosoi, Trichophytoon rubrum, Microsporium canis and M. gypseum.

References

  1. Maa X, Youa P, Xub Y, Yea X, Tua Y, Liua Y et al. Anti-Helicobacter pylori-associated gastritis effect of the ethyl acetate extract of Alpinia officinarum Hance through MAPK signaling pathway. J Ethnopharmacol. 2020; 260: 113100. [CrossRef].
  2. Sharifi-Rada J, Salehib B, Stojanović-Radićc ZZ, Fokoud PVT, Sharifi-Radf M, Mahadyg GB et al. Medicinal plants used in the treatment of tuberculosis-Ethnobotanical and ethnopharmacological approaches. Biotechnol Adv. 2020; 44: 107629. [CrossRef].
  3. Wannissorn B, Jarikasem S, Siriwangchai T, Thubthimthed S. Antibacterial properties of essential oils from Thai medicinal plants. Fitoterapia. 2005; 76(2): 233-6. [CrossRef].
  4. Tushar, Basak S, Sarma GC, Rangan L. Ethnomedical uses of Zingiberaceous plants of Northeast India. J Ethnopharmacol. 2010; 132(1): 286-96. [CrossRef].
  5. Mathew S, Victório CP. Antifungal properties of rhizomes of Alpinia calcarata Roscoe from Western Ghats, South India. Int J Pharm Phytopharm Res (eIJPPR). 2020; 10(5): 1-7.
  6. Kress WJ, Liu AZ, Newman M, Li QJ. The molecular phylogeny of Alpinia (Zingiberaceae): A complex polyphyletic genus of Gingers. Amer J Bot. 2005; 92: 167-78. [CrossRef].
  7. Victório CP, Leitão SG, Lage CLS. Chemical composition of the leaf oils of Alpinia zerumbet (Pers.) Burtt et Smith and A. purpurata (Vieill) K. Schum. from Rio de Janeiro, Brazil. J Essent Oil Res. 2010; 22: 52-4. [CrossRef].
  8. Victório CP. Therapeutic value of the genus Alpinia, Zingiberaceae. Braz J Pharmacogn. 2011; 21(1): 194-201. ISSN 1981-528X. [CrossRef].
  9. Hsu SY. Effects of the constituents of Alpinia speciosa rhizome on experimental ulcers. Taiwan Yi Xue Hui Za Zhi. 1987; 86(1): 58-64. [PubMed].
  10. Victório CP, Kuster RM, Moura RS, Lage CLS. Vasodilator activity of extracts of field Alpinia purpurata (Vieill) K: Schum and A. zerumbet (Pers.) Burtt et Smith cultured in vitro. Braz J Pharma Sci. 2009; 45(3): 507-514. [CrossRef].
  11. Ghareeb M, Sobeh M, Rezq S, El-Shazly A, Mahmoud M, Wink M. HPLC-ESI-MS/MS profiling of polyphenolics of a leaf extract from Alpinia zerumbet (Zingiberaceae) and its anti-Inflammatory, anti-Nociceptive, and antipyretic activities in vivo. Molecules. 2018; 23(12): 3238. [CrossRef] [PubMed].
  12. Ferreira GRS, Brito JS, Procópio TF, Santos NDL, Lima BJRC, Coelho LCBB et al. Antimicrobial potential of Alpinia purpurata lectin (ApuL): growth inhibitory action, synergistic effects in combination with antibiotics, and antibiofilm activity. Microb Pathog. 2018; 124:152-162. [CrossRef] [PubMed].
  13. Cruz JD, Mpalantinos MA, Ramos AS, Ferreira JLP, Oliveira AA, Netto Júnior NL et al. Chemical standardization, antioxidant activity and phenolic contents of cultivated Alpinia zerumbet preparations. Ind Crops Prod. 2020; 151(1): 112495. [CrossRef].
  14. Victório CP, Alviano DN, Alviano CS, Lage CLS. Chemical composition of the fractions of leaf oil of Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm. and antimicrobial activity. Braz J Pharmacogn. 2009; 19(3):697-701. ISSN 1981-528X. [CrossRef].
  15. Kongkham B, Prabakaran D, Puttaswamy H. Opportunities and challenges in managing antibiotic resistance in bacteria using plant secondary metabolites. Fitoterapia. 2020; 104762. [CrossRef].
  16. Bilal M, Rasheed T, Iqbal HMN, Hu H, Wang W, Zhang X. Macromolecular agents with antimicrobial potentialities: A drive to combat antimicrobial resistance. Int J Biol Macromolecules. 2017; 103: 554-74 [CrossRef] [PubMed].
  17. Aslam B, Wang W, Arshad MI, Khurshid M, Muzammil S, Rasool MH et al. Antibiotic resistance: a rundown of a global crisis. Infect Drug Resist. 2018; 11:1645-58. [CrossRef].
  18. Adams RP. Identification of essential oil components by gas chromatography/mass spectrometry. 5th ed. Gruver: Texensis Publishing, 2017. ISBN: 978-0-9981557-2-2.
  19. Hili P, Evans CS, Verness RG. Antimicrobial action of essential oils: the effect of dimethylsulphoxide on the activity of cinnamon oil. Lett Appl Microbiol. 1997; 24(4): 269-75. [CrossRef].
  20. Mpalatinos MA, Soares de Moura R, Parente JP, Kuster RM. Biologically active flavonoids and kava pyrones from the aqueous extract of Alpinia zerumbet. Phytotherapy. 1998; 12(6): 442-4. [CrossRef].
  21. Victório CP, Lage CLS, Kuster RM. Flavonoid extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different techniques and solvents. Eclet Quím. 2009; 34(1): 19-24. ISSN 1678-4618. [CrossRef].
  22. Agoramoorthy G, Chandrasekaran M, Venkatesalu V, Hsu MJ. Antibacterial and antifungal activities of fatty acid methyl esters of the blind-your-eye mangrove from India. Braz J Microbiol. 2007; 38(4): 739-742. ISSN 1678-4405. [CrossRef].
  23. Marquis RE, Clock SA, Mota-Meira M. Fluoride and organic weak acids as modulators of microbial physiology. FEMS Microbiol Rev. 2003; 26(5): 493-510. [CrossRef].
  24. Nalina T, Rahim ZHA. The crude aqueous extract of Piper betle L. and its antibacterial effect towards Streptococcus mutans. Amer J Biotechnol Biochem. 2007; 3(1): 10-5. [CrossRef].
  25. Kumar P, Lee J-H, Beyenal H, Lee J. Fatty acids as antibiofilm and antivirulence agents. Trends Microbiol. 2020; 28(9): 753-768. [CrossRef].
  26. Habsah M, Amran M, Mackeen MM, Lajis NH, Kikuzaki H, Nakatani N et al. Screening of Zingiberaceae extracts for antimicrobial and antioxidant activities. J Ethnopharmacol. 2000; 72(3): 403-10. ISSN 0378-8741. [CrossRef].
  27. Kochuthressia KP, Britto SJ, Jaseentha MO, Raj JM, Senthikumar SR. Antimicrobial efficacy of extracts from Alpinia purpurata (Vieill.) K. Schum. against human pathogenic bacteria and fungi. ABJNA. 2010; 1(6):1249-52. ISSN 2151-7525. [CrossRef].
  28. Victório CP, Kuster RM, Lage CLS. Detection of flavonoids in Alpinia purpurata (Vieill) K. Schum. leaves by high-performance liquid chromatography. Braz J Med Plants. 2009; 11(2): 147-53. ISSN 1983-084X. [CrossRef].
  29. Wang Y, Huang TL. Screening of anti-Helicobacter pylori herbs deriving from Taiwanese folk medicinal plants. FEMS Immunol Med Microbiol. 2005; 43(2): 295-300. [CrossRef].
  30. Masuda T, Jitoe A. Antioxidative and antiinflammatory compounds from tropical gingers: isolation, structure determination, and activities of cassumins A, B and C, new complex curcuminoids from Zingiber cassumunar. J Agric Food Chemistry. 1994; 42(9):1850-6. [CrossRef].
  31. Tawata S, Taira S, Kobamoto N, Ishihara M, Toyama S. Syntheses and biological activities of dihydro-5,6-dehydrokawain derivatives. Biosci Biotechnol Biochem. 1996; 60(10):1643-5. [CrossRef] [PubMed].
  32. Huang CB, Alimova Y, Myers TM, Ebersole JL. Short- and medium-chain fatty acids exhibit antimicrobial activity for oral microorganisms. Arch Oral Biol. 2011; 56(7): 650-654. [CrossRef].
  33. Abubakar MN, Majinda RRT. GC-MS Analysis and Preliminary Antimicrobial Activity of Albizia adianthifolia (Schumach) and Pterocarpus angolensis (DC). Medicines. 2016; 3(1): 3. [CrossRef].
  34. Liu S, Ruan W, Li J, Xu H. Biological control of phytopathogenic fungi by fatty acids. Mycopathologia. 2008; 166(2): 93-102. [CrossRef] [PubMed].
  35. Kabara JJ, Swieczkowski DM, Conley AJ, Truant JP. (1972). Fatty acids and derivatives as antimicrobial agents. Antimicrob Agents Chemother. 1972; 2: 23-28.
PDF
HTML
XML
MP3
Save bookmark

Author(s)

  • Cristiane Pimentel Victorio
    Centro Universitário Estadual da Zona Oeste (UEZO), Colegiado de Ciências Biológicas e da Saúde - CCBS. Avenida Manuel Caldeira de Alvarenga, 1203 Campo Grande, CEP 23070-200, Rio de Janeiro, RJ, Brasil.
  • Davi Oliveira e Silva
    Universidade Federal de Ouro Preto (UFOP), Department of Biodiversity, Evolution and Environment-DEBIO, Institute of Exact and Biological Sciences (ICEB), Morro do Cruzeiro campus, CEP 35400-000, Ouro Preto, MG, Brazil.
  • Daniela Alviano
    Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, s / n, 21941-902 Rio de Janeiro-RJ, Brazil
  • Celuta Alviano
    Paulo de Góes Institute of Microbiology, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, s / n, 21941-902 Rio de Janeiro-RJ, Brazil
  • Ricardo Machado Kuster
    Federal University of Espírito Santo (UFES), Department of Chemistry, Av. Fernando Ferrari, 514, Goiabeiras, CEP 29075-910, Vitória, ES, Brazil
  • Celso Luiz Salgueiro Lage
    National Institute of Industrial Property (INPI), DICOD - Academia. Praça Mauá 7, Sala 1012, Centro, CEP 20081-240, Rio de Janeiro, RJ, Brazil

Metrics

  • Article viewed 469 time(s)

How to Cite

1.
In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract. Rev Fitos [Internet]. 2021 Jun. 30 [cited 2024 Nov. 26];15(2):136-43. Available from: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1037
Crossref
Scopus
Google Scholar
Europe PMC
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2019 Revista Fitos

Report an error